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Protein

Cytochrome P450 4V2

Gene

Cyp4v2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Omega-hydroxylase that oxidizes medium-chain saturated fatty acids and polyunsaturated omega-3 fatty acids, and which plays a role in fatty acid and steroid metabolism in the eye. Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). Acts as a polyunsaturated omega-3 fatty acids hydroxylase by mediating oxidation of docosahexaenoate (DHA) to 22-hydroxydocosahexaenoate. Also produces some 21-hydroxydocosahexaenoate. Also converts eicosapentaenoate (EPA) to 20-hydroxyeicosapentaenoate (20-OH-EPA).By similarity

Catalytic activityi

Docosahexaenoate + [reduced NADPH--hemoprotein reductase] + O2 = 22-hydroxydocosahexaenoate + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Activity regulationi

Inhibited by N-hydroxy-N'-(4-n-butyl-2-methylphenyl formamidine)(HET0016) with an IC50 of 38 nM.By similarity

Pathwayi: Lipid metabolism

This protein is involved in Lipid metabolism.Curated
View all proteins of this organism that are known to be involved in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei329Heme (covalent; via 1 link)By similarity1
Metal bindingi467Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-211976 Endogenous sterols
R-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4V2 (EC:1.14.14.-)
Alternative name(s):
Docosahexaenoic acid omega-hydroxylase CYP4V2Curated (EC:1.14.14.79By similarity)
Gene namesi
Name:Cyp4v2
Synonyms:Cyp4v3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2142763 Cyp4v3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518611 – 525Cytochrome P450 4V2Add BLAST525

Proteomic databases

MaxQBiQ9DBW0
PaxDbiQ9DBW0
PRIDEiQ9DBW0

PTM databases

iPTMnetiQ9DBW0
PhosphoSitePlusiQ9DBW0
SwissPalmiQ9DBW0

Expressioni

Gene expression databases

BgeeiENSMUSG00000079057 Expressed in 200 organ(s), highest expression level in jejunum
ExpressionAtlasiQ9DBW0 baseline and differential
GenevisibleiQ9DBW0 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092966

Structurei

3D structure databases

ProteinModelPortaliQ9DBW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140829
HOVERGENiHBG000182
InParanoidiQ9DBW0
KOiK07427
OMAiYKLHQSY
OrthoDBiEOG091G06KN
PhylomeDBiQ9DBW0
TreeFamiTF105088

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DBW0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLWLWLGLSG QKLLLWGAAS AVSLAGATIL ISIFPMLVSY ARKWQQMRSI
60 70 80 90 100
PSVARAYPLV GHALYMKPNN AEFFQQLIYY TEEFRHLPII KLWIGPVPLV
110 120 130 140 150
ALYKAENVEV ILTSSKQIDK SFLYKFLQPW LGLGLLTSTG SKWRTRRKML
160 170 180 190 200
TPTFHFTILE NFLDVMNEQA NILVNKLEKH VNQEAFNCFF YITLCALDII
210 220 230 240 250
CETAMGKNIG AQSNNDSEYV RTVYRMSDMI YRRMKMPWLW FDLWYLVFKE
260 270 280 290 300
GRDHKRGLKC LHTFTNNVIA ERVKERKAEE DWTGAGRGPI PSKNKRKAFL
310 320 330 340 350
DLLLSVTDEE GNRLSQEDIR EEVDTFMFEG HDTTAAAINW SLYLLGTNPE
360 370 380 390 400
VQRKVDQELD EVFGRSHRPV TLEDLKKLKY LDCVIKETLR VFPSVPLFAR
410 420 430 440 450
SLSEDCEVGG YKVTKGTEAI IIPYALHRDP RYFPDPEEFR PERFFPENSQ
460 470 480 490 500
GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI LACILRQFWV ESNQKREELG
510 520
LAGDLILRPN NGIWIKLKRR HEDDP
Length:525
Mass (Da):60,939
Last modified:June 1, 2001 - v1
Checksum:i71BB341589BD10A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056457 mRNA Translation: BAB33032.1
AK004724 mRNA Translation: BAB23507.1
CCDSiCCDS22276.1
RefSeqiNP_598730.1, NM_133969.2
UniGeneiMm.245297

Genome annotation databases

EnsembliENSMUST00000095328; ENSMUSP00000092966; ENSMUSG00000079057
GeneIDi102294
KEGGimmu:102294
UCSCiuc009lou.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056457 mRNA Translation: BAB33032.1
AK004724 mRNA Translation: BAB23507.1
CCDSiCCDS22276.1
RefSeqiNP_598730.1, NM_133969.2
UniGeneiMm.245297

3D structure databases

ProteinModelPortaliQ9DBW0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092966

PTM databases

iPTMnetiQ9DBW0
PhosphoSitePlusiQ9DBW0
SwissPalmiQ9DBW0

Proteomic databases

MaxQBiQ9DBW0
PaxDbiQ9DBW0
PRIDEiQ9DBW0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095328; ENSMUSP00000092966; ENSMUSG00000079057
GeneIDi102294
KEGGimmu:102294
UCSCiuc009lou.1 mouse

Organism-specific databases

CTDi102294
MGIiMGI:2142763 Cyp4v3

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140829
HOVERGENiHBG000182
InParanoidiQ9DBW0
KOiK07427
OMAiYKLHQSY
OrthoDBiEOG091G06KN
PhylomeDBiQ9DBW0
TreeFamiTF105088

Enzyme and pathway databases

ReactomeiR-MMU-211976 Endogenous sterols
R-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)

Miscellaneous databases

PROiPR:Q9DBW0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079057 Expressed in 200 organ(s), highest expression level in jejunum
ExpressionAtlasiQ9DBW0 baseline and differential
GenevisibleiQ9DBW0 MM

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP4V2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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