UniProtKB - Q9DBT4 (ENTP4_MOUSE)
Protein
Ectonucleoside triphosphate diphosphohydrolase 4
Gene
Entpd4
Organism
Mus musculus (Mouse)
Status
Functioni
Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent.By similarity
Catalytic activityi
- EC:3.6.1.6
Cofactori
Ca2+By similarity, Mg2+By similarityNote: Requires Ca2+ and can also use Mg2+, but with lower efficiency.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 222 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- uridine-diphosphatase activity Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, Magnesium |
Enzyme and pathway databases
Reactomei | R-MMU-8850843, Phosphate bond hydrolysis by NTPDase proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Ectonucleoside triphosphate diphosphohydrolase 4 (EC:3.6.1.6)Short name: NTPDase 4 Alternative name(s): Lysosomal apyrase-like protein of 70 kDa Uridine-diphosphatase Short name: UDPase |
Gene namesi | Name:Entpd4 Synonyms:Lalp70, Lysal1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914714, Entpd4 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Other locations
- autophagosome membrane By similarity; Multi-pass membrane protein By similarity
Note: Localizes in the Golgi and autophagic vacuoles/lysosomes.By similarity
Golgi apparatus
- integral component of Golgi membrane Source: MGI
Other locations
- cytoplasmic vesicle Source: UniProtKB-KW
- integral component of autophagosome membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 559 | LumenalSequence analysisAdd BLAST | 505 | |
Transmembranei | 560 – 580 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 581 – 613 | CytoplasmicSequence analysisAdd BLAST | 33 |
Keywords - Cellular componenti
Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209912 | 1 – 613 | Ectonucleoside triphosphate diphosphohydrolase 4Add BLAST | 613 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 404 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 407 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 461 ↔ 490 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9DBT4 |
PRIDEi | Q9DBT4 |
PTM databases
GlyGeni | Q9DBT4, 2 sites |
PhosphoSitePlusi | Q9DBT4 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000022066, Expressed in quadriceps femoris and 52 other tissues |
ExpressionAtlasi | Q9DBT4, baseline |
Genevisiblei | Q9DBT4, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000139202 |
Miscellaneous databases
RNActi | Q9DBT4, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the GDA1/CD39 NTPase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01010000222402 |
HOGENOMi | CLU_010246_6_0_1 |
InParanoidi | Q9DBT4 |
OMAi | IQQENFR |
OrthoDBi | 1337265at2759 |
PhylomeDBi | Q9DBT4 |
TreeFami | TF354343 |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9DBT4-1) [UniParc]FASTAAdd to basket
Also known as: LALP70
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIVVISIL AAAVSLLYFS
60 70 80 90 100
VVIIRSKYGW LSKDKKFQRY LARVTDVEAT DTNNPSVNYG IVVDCGSSGS
110 120 130 140 150
RIFVYCWPRH NGNPHDLLDI RQMRDKNRKP VVMKIKPGIS EFATSPEKVS
160 170 180 190 200
DYISPLLSFA AEHVPRAKHK ETPLYILCTA GMRVLPESQQ KAILEDLLTD
210 220 230 240 250
IPVHYDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI EEDDEAVVEV
260 270 280 290 300
NIPGSESSEA IVRKRTAGVL DMGGVSTQIA YEVPQTVSFA SSQQEEVAKN
310 320 330 340 350
LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR LFASTVQKNR
360 370 380 390 400
LLGKQTGLTP DAPLLDPCLP LDIKDEIQQN GQTLYLQGTG DFDLCRETLQ
410 420 430 440 450
PFMNKTNETQ TSLNGVYQPP IHFQNSEFYG FSEFYYCTED VLRMGGDYNA
460 470 480 490 500
ARFTQAAKDY CATKWSILRE RFDRGLYASH ADLHRLKYQC FKSAWMFEVF
510 520 530 540 550
HKGFSFPVTY KNLKTALQVY DKEVQWTLGA ILYRTRFLPL RDIRQEVFRA
560 570 580 590 600
GHAHWRGVSF VYNHYLFSGC FLVVLLSILL YLLRLRRIHR RAPRTGSLWM
610
EEGLPSQKGP GPL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ80WR8 | Q80WR8_MOUSE | Ectonucleoside triphosphate diphosp... | Entpd4 Entpd4b | 102 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003615 | 287 – 294 | Missing in isoform 2. 1 Publication | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004761 mRNA Translation: BAB23542.1 BC006924 mRNA Translation: AAH06924.1 BC043134 mRNA Translation: AAH43134.1 |
CCDSi | CCDS27240.1 [Q9DBT4-1] |
RefSeqi | NP_080450.1, NM_026174.3 [Q9DBT4-1] XP_003688976.1, XM_003688928.3 XP_003688977.1, XM_003688929.4 XP_006519516.1, XM_006519453.2 |
Genome annotation databases
Ensembli | ENSMUST00000064831; ENSMUSP00000065046; ENSMUSG00000095463 [Q9DBT4-1] ENSMUST00000184973; ENSMUSP00000138944; ENSMUSG00000095463 [Q9DBT4-1] ENSMUST00000185072; ENSMUSP00000139202; ENSMUSG00000022066 [Q9DBT4-1] |
GeneIDi | 67464 |
KEGGi | mmu:67464 |
UCSCi | uc007umi.2, mouse [Q9DBT4-1] uc007umk.2, mouse [Q9DBT4-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004761 mRNA Translation: BAB23542.1 BC006924 mRNA Translation: AAH06924.1 BC043134 mRNA Translation: AAH43134.1 |
CCDSi | CCDS27240.1 [Q9DBT4-1] |
RefSeqi | NP_080450.1, NM_026174.3 [Q9DBT4-1] XP_003688976.1, XM_003688928.3 XP_003688977.1, XM_003688929.4 XP_006519516.1, XM_006519453.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000139202 |
PTM databases
GlyGeni | Q9DBT4, 2 sites |
PhosphoSitePlusi | Q9DBT4 |
Proteomic databases
PaxDbi | Q9DBT4 |
PRIDEi | Q9DBT4 |
Genome annotation databases
Ensembli | ENSMUST00000064831; ENSMUSP00000065046; ENSMUSG00000095463 [Q9DBT4-1] ENSMUST00000184973; ENSMUSP00000138944; ENSMUSG00000095463 [Q9DBT4-1] ENSMUST00000185072; ENSMUSP00000139202; ENSMUSG00000022066 [Q9DBT4-1] |
GeneIDi | 67464 |
KEGGi | mmu:67464 |
UCSCi | uc007umi.2, mouse [Q9DBT4-1] uc007umk.2, mouse [Q9DBT4-2] |
Organism-specific databases
CTDi | 9583 |
MGIi | MGI:1914714, Entpd4 |
Phylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01010000222402 |
HOGENOMi | CLU_010246_6_0_1 |
InParanoidi | Q9DBT4 |
OMAi | IQQENFR |
OrthoDBi | 1337265at2759 |
PhylomeDBi | Q9DBT4 |
TreeFami | TF354343 |
Enzyme and pathway databases
Reactomei | R-MMU-8850843, Phosphate bond hydrolysis by NTPDase proteins |
Miscellaneous databases
BioGRID-ORCSi | 100862375, 0 hits in 2 CRISPR screens 67464, 0 hits in 15 CRISPR screens |
ChiTaRSi | Entpd4, mouse |
PROi | PR:Q9DBT4 |
RNActi | Q9DBT4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022066, Expressed in quadriceps femoris and 52 other tissues |
ExpressionAtlasi | Q9DBT4, baseline |
Genevisiblei | Q9DBT4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTP4_MOUSE | |
Accessioni | Q9DBT4Primary (citable) accession number: Q9DBT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 29, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families