UniProtKB - Q9DBL7 (COASY_MOUSE)
Protein
Bifunctional coenzyme A synthase
Gene
Coasy
Organism
Mus musculus (Mouse)
Status
Functioni
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.By similarity
Catalytic activityi
: coenzyme A biosynthesis Pathwayi
This protein is involved in step 4 and 5 of the subpathway that synthesizes CoA from (R)-pantothenate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Pantothenate kinase 3 (Pank3), Pantothenate kinase 1 (Pank1)
- Phosphopantothenate--cysteine ligase (Ppcs)
- Phosphopantothenoylcysteine decarboxylase (Ppcdc)
- Bifunctional coenzyme A synthase (Coasy)
- Bifunctional coenzyme A synthase (Coasy)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 364 – 371 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- dephospho-CoA kinase activity Source: MGI
- pantetheine-phosphate adenylyltransferase activity Source: MGI
GO - Biological processi
- coenzyme A biosynthetic process Source: MGI
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase |
Biological process | Coenzyme A biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-196783, Coenzyme A biosynthesis |
UniPathwayi | UPA00241;UER00355 UPA00241;UER00356 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional coenzyme A synthaseShort name: CoA synthase Including the following 2 domains: |
Gene namesi | Name:Coasy Synonyms:Ukr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918993, Coasy |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Other locations
- Cytoplasm By similarity
Note: The protein is mainly present in the mitochondrial matrix, probably anchored to the inner mitochondrial membrane, but this protein is also present in cell lysate.By similarity
Mitochondrion
- mitochondrial matrix Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 203 | H → A: Phosphopantetheine adenylyltransferase activity abolished. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173040 | 1 – 563 | Bifunctional coenzyme A synthaseAdd BLAST | 563 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 177 | PhosphoserineBy similarity | 1 | |
Modified residuei | 182 | PhosphoserineBy similarity | 1 | |
Modified residuei | 461 | N6-succinyllysineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9DBL7 |
jPOSTi | Q9DBL7 |
MaxQBi | Q9DBL7 |
PaxDbi | Q9DBL7 |
PeptideAtlasi | Q9DBL7 |
PRIDEi | Q9DBL7 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9DBL7 |
PTM databases
iPTMneti | Q9DBL7 |
PhosphoSitePlusi | Q9DBL7 |
SwissPalmi | Q9DBL7 |
Expressioni
Tissue specificityi
Widely expressed with highest levels in kidney and lowest levels in colon, lung, intestine, and spleen.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000001755, Expressed in adult mammalian kidney and 305 other tissues |
Genevisiblei | Q9DBL7, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 214894, 5 interactors |
IntActi | Q9DBL7, 2 interactors |
MINTi | Q9DBL7 |
STRINGi | 10090.ENSMUSP00000102929 |
Miscellaneous databases
RNActi | Q9DBL7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9DBL7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9DBL7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 359 – 562 | DPCKAdd BLAST | 204 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 179 – 357 | Phosphopantetheine adenylyltransferaseAdd BLAST | 179 |
Sequence similaritiesi
In the central section; belongs to the eukaryotic CoaD family.Curated
Phylogenomic databases
eggNOGi | KOG3220, Eukaryota KOG3351, Eukaryota |
GeneTreei | ENSGT00550000075078 |
HOGENOMi | CLU_027827_1_0_1 |
InParanoidi | Q9DBL7 |
OMAi | VIDCDKV |
OrthoDBi | 1543581at2759 |
PhylomeDBi | Q9DBL7 |
TreeFami | TF105783 |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00376, Dephospho_CoA_kinase, 1 hit |
InterProi | View protein in InterPro IPR004821, Cyt_trans-like IPR001977, Depp_CoAkinase IPR027417, P-loop_NTPase IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01121, CoaE, 1 hit PF01467, CTP_transf_like, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00152, TIGR00152, 1 hit |
PROSITEi | View protein in PROSITE PS51219, DPCK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9DBL7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVFRSGLLV LTTPLATLAA RLPPILTSAS RLVNHTLYVH LQPGMNLGGP
60 70 80 90 100
AQPQASPVQA TFEVLDFITH LYTGADLHRH LDVRILLTNI QTKSTFLPVL
110 120 130 140 150
SSVQNLAHPP EVVLTDFQTL DGSQYNPVKQ QLERYATSCY SCSPQLASVL
160 170 180 190 200
LYPDYGTGEL PLEPPNALLP STIRPASPVA RSPRQPVRGY HRGAVGGTFD
210 220 230 240 250
RLHNAHKVLL SVACVLAQEQ LVVGVADKDL LKSKLLPELL QPYAERVEHL
260 270 280 290 300
TEFLVDIKPS LTFELVPLLD PYGPAGSDPT LEFLVVSEET YRGGMAVNRF
310 320 330 340 350
RLENGKEELA LYQIQLLKDQ SHNENEEDKV SSSSFRQRIL GNLLQPPNER
360 370 380 390 400
PELPSGLYVL GLTGISGSGK SSVAQRLKNL GAYIIDSDHL GHRAYAPGGP
410 420 430 440 450
AYQPVVEAFG TDILHKDGTI NRKVLGSRVF GNKKQMKILT DIVWPVIAKL
460 470 480 490 500
AREEMDVAVA KGKTLCVIDA AMLLEAGWQS MVHEVWTVVI PETEAVRRIV
510 520 530 540 550
ERDGLSEAAA QSRLQSQMSG QQLVEQSNVV LSTLWESHVT QSQVEKAWNL
560
LQKRLPKAYQ TRN
Sequence cautioni
The sequence AAH20046 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF521095 mRNA Translation: AAM77669.1 AK004878 mRNA Translation: BAB23636.2 AK159405 mRNA Translation: BAE35057.1 AK159912 mRNA Translation: BAE35475.1 AK166894 mRNA Translation: BAE39099.1 BX255926 Genomic DNA No translation available. BC020046 mRNA Translation: AAH20046.1 Different initiation. |
CCDSi | CCDS25446.1 |
RefSeqi | NP_001292911.1, NM_001305982.1 XP_006534320.1, XM_006534257.2 XP_017170260.1, XM_017314771.1 |
Genome annotation databases
Ensembli | ENSMUST00000001806; ENSMUSP00000001806; ENSMUSG00000001755 ENSMUST00000107308; ENSMUSP00000102929; ENSMUSG00000001755 |
GeneIDi | 71743 |
KEGGi | mmu:71743 |
UCSCi | uc007lnc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF521095 mRNA Translation: AAM77669.1 AK004878 mRNA Translation: BAB23636.2 AK159405 mRNA Translation: BAE35057.1 AK159912 mRNA Translation: BAE35475.1 AK166894 mRNA Translation: BAE39099.1 BX255926 Genomic DNA No translation available. BC020046 mRNA Translation: AAH20046.1 Different initiation. |
CCDSi | CCDS25446.1 |
RefSeqi | NP_001292911.1, NM_001305982.1 XP_006534320.1, XM_006534257.2 XP_017170260.1, XM_017314771.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2F6R | X-ray | 1.70 | A | 295-563 | [»] | |
SMRi | Q9DBL7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 214894, 5 interactors |
IntActi | Q9DBL7, 2 interactors |
MINTi | Q9DBL7 |
STRINGi | 10090.ENSMUSP00000102929 |
PTM databases
iPTMneti | Q9DBL7 |
PhosphoSitePlusi | Q9DBL7 |
SwissPalmi | Q9DBL7 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9DBL7 |
Proteomic databases
EPDi | Q9DBL7 |
jPOSTi | Q9DBL7 |
MaxQBi | Q9DBL7 |
PaxDbi | Q9DBL7 |
PeptideAtlasi | Q9DBL7 |
PRIDEi | Q9DBL7 |
Protocols and materials databases
Antibodypediai | 16973, 297 antibodies |
DNASUi | 71743 |
Genome annotation databases
Ensembli | ENSMUST00000001806; ENSMUSP00000001806; ENSMUSG00000001755 ENSMUST00000107308; ENSMUSP00000102929; ENSMUSG00000001755 |
GeneIDi | 71743 |
KEGGi | mmu:71743 |
UCSCi | uc007lnc.1, mouse |
Organism-specific databases
CTDi | 80347 |
MGIi | MGI:1918993, Coasy |
Phylogenomic databases
eggNOGi | KOG3220, Eukaryota KOG3351, Eukaryota |
GeneTreei | ENSGT00550000075078 |
HOGENOMi | CLU_027827_1_0_1 |
InParanoidi | Q9DBL7 |
OMAi | VIDCDKV |
OrthoDBi | 1543581at2759 |
PhylomeDBi | Q9DBL7 |
TreeFami | TF105783 |
Enzyme and pathway databases
UniPathwayi | UPA00241;UER00355 UPA00241;UER00356 |
Reactomei | R-MMU-196783, Coenzyme A biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 71743, 5 hits in 13 CRISPR screens |
ChiTaRSi | Coasy, mouse |
EvolutionaryTracei | Q9DBL7 |
PROi | PR:Q9DBL7 |
RNActi | Q9DBL7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001755, Expressed in adult mammalian kidney and 305 other tissues |
Genevisiblei | Q9DBL7, MM |
Family and domain databases
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00376, Dephospho_CoA_kinase, 1 hit |
InterProi | View protein in InterPro IPR004821, Cyt_trans-like IPR001977, Depp_CoAkinase IPR027417, P-loop_NTPase IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF01121, CoaE, 1 hit PF01467, CTP_transf_like, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00152, TIGR00152, 1 hit |
PROSITEi | View protein in PROSITE PS51219, DPCK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COASY_MOUSE | |
Accessioni | Q9DBL7Primary (citable) accession number: Q9DBL7 Secondary accession number(s): A2BFA8, Q3TVZ2, Q8K3Y4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | June 27, 2003 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families