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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • protein N-linked glycosylation Source: MGI
  • response to drug Source: Ensembl

Enzyme and pathway databases

UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:Rpn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98085 Rpn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000002224823 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9DBG6
MaxQBiQ9DBG6
PaxDbiQ9DBG6
PeptideAtlasiQ9DBG6
PRIDEiQ9DBG6
TopDownProteomicsiQ9DBG6

PTM databases

iPTMnetiQ9DBG6
PhosphoSitePlusiQ9DBG6
SwissPalmiQ9DBG6

Expressioni

Gene expression databases

BgeeiENSMUSG00000027642 Expressed in 317 organ(s), highest expression level in embryo
CleanExiMM_RPN2
ExpressionAtlasiQ9DBG6 baseline and differential
GenevisibleiQ9DBG6 MM

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi202990, 5 interactors
IntActiQ9DBG6, 12 interactors
MINTiQ9DBG6
STRINGi10090.ENSMUSP00000112081

Structurei

3D structure databases

SMRiQ9DBG6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOVERGENiHBG002365
InParanoidiQ9DBG6
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
TreeFamiTF106146

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9DBG6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPGSSAVF LLALTITASV QALTPTHYLT KQDVERLKAS LDRPFTDLES
60 70 80 90 100
AFYSIVGLSS LGVQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQVLSGC
110 120 130 140 150
EISVSNETKE LLLAAVSEDS PIAQIYHAVA ALSGFGLPLA SNEALGALTA
160 170 180 190 200
RLGKEETVLA TVQALQTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLELTAL FVAATYKLMD HVGTEPSMKE DQVIQLMNTI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA ALSQNRYHVP VVVVPEGSTS DTQEQAILRL QVSNVLSQPL
310 320 330 340 350
AQAAVKLEHA KSAATRATVL QKTPFSLVGN VFELNFKNVK LSSGYYDFSV
360 370 380 390 400
RVEGDSRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQSLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YIYWTQLNMF
610 620 630
QTLKYLAVLG TVTFLAGNRM LAQHAVKRTA H
Length:631
Mass (Da):69,063
Last modified:June 1, 2001 - v1
Checksum:iD20745D10D5486F9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ACG7A2ACG7_MOUSE
Dolichyl-diphosphooligosaccharide--...
Rpn2
615Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA Translation: BAB23707.1
AK161624 mRNA Translation: BAE36498.1
AK166778 mRNA Translation: BAE39013.1
AL669828 Genomic DNA No translation available.
BC010509 mRNA Translation: AAH10509.1
BC046806 mRNA Translation: AAH46806.1
CCDSiCCDS16975.1
RefSeqiNP_062616.2, NM_019642.4
UniGeneiMm.22130

Genome annotation databases

EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642
GeneIDi20014
KEGGimmu:20014
UCSCiuc008now.1 mouse

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA Translation: BAB23707.1
AK161624 mRNA Translation: BAE36498.1
AK166778 mRNA Translation: BAE39013.1
AL669828 Genomic DNA No translation available.
BC010509 mRNA Translation: AAH10509.1
BC046806 mRNA Translation: AAH46806.1
CCDSiCCDS16975.1
RefSeqiNP_062616.2, NM_019642.4
UniGeneiMm.22130

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J6GX-ray3.30E/F373-380[»]
5J6HX-ray2.30F373-380[»]
SMRiQ9DBG6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202990, 5 interactors
IntActiQ9DBG6, 12 interactors
MINTiQ9DBG6
STRINGi10090.ENSMUSP00000112081

PTM databases

iPTMnetiQ9DBG6
PhosphoSitePlusiQ9DBG6
SwissPalmiQ9DBG6

Proteomic databases

EPDiQ9DBG6
MaxQBiQ9DBG6
PaxDbiQ9DBG6
PeptideAtlasiQ9DBG6
PRIDEiQ9DBG6
TopDownProteomicsiQ9DBG6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642
GeneIDi20014
KEGGimmu:20014
UCSCiuc008now.1 mouse

Organism-specific databases

CTDi6185
MGIiMGI:98085 Rpn2

Phylogenomic databases

eggNOGiKOG2447 Eukaryota
ENOG410XRF2 LUCA
GeneTreeiENSGT00390000002635
HOVERGENiHBG002365
InParanoidiQ9DBG6
KOiK12667
OMAiPKPEIQH
OrthoDBiEOG091G0H3U
TreeFamiTF106146

Enzyme and pathway databases

UniPathwayi
UPA00378

Miscellaneous databases

ChiTaRSiRpn2 mouse
PROiPR:Q9DBG6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027642 Expressed in 317 organ(s), highest expression level in embryo
CleanExiMM_RPN2
ExpressionAtlasiQ9DBG6 baseline and differential
GenevisibleiQ9DBG6 MM

Family and domain databases

InterProiView protein in InterPro
IPR008814 Swp1
PANTHERiPTHR12640 PTHR12640, 1 hit
PfamiView protein in Pfam
PF05817 Ribophorin_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBG6
Secondary accession number(s): A2ACG6, Q3TKY0, Q91XH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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