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Protein

Sterol 26-hydroxylase, mitochondrial

Gene

Cyp27a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.By similarity

Catalytic activityi

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 6 reduced adrenodoxin + 6 H+ + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 6 oxidized adrenodoxin + 4 H2O.By similarity

Cofactori

hemeBy similarity

Pathwayi: cholecalciferol biosynthesis

This protein is involved in the pathway cholecalciferol biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholecalciferol biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi479Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-MMU-211976 Endogenous sterols
UniPathwayi
UPA00955

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 26-hydroxylase, mitochondrial (EC:1.14.15.15By similarity)
Alternative name(s):
5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase
Cytochrome P-450C27/25
Cytochrome P450 27
Sterol 27-hydroxylase
Vitamin D(3) 25-hydroxylase
Gene namesi
Name:Cyp27a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88594 Cyp27a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionBy similarityAdd BLAST32
ChainiPRO_000000361933 – 533Sterol 26-hydroxylase, mitochondrialAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei142N6-acetyllysineCombined sources1
Modified residuei232N6-acetyllysineCombined sources1
Modified residuei285N6-acetyllysineCombined sources1
Modified residuei296N6-acetyllysineCombined sources1
Modified residuei375N6-acetyllysineCombined sources1
Modified residuei512N6-acetyllysineCombined sources1
Modified residuei523N6-acetyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-125 and Lys-285 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9DBG1
PaxDbiQ9DBG1
PeptideAtlasiQ9DBG1
PRIDEiQ9DBG1

PTM databases

iPTMnetiQ9DBG1
PhosphoSitePlusiQ9DBG1
SwissPalmiQ9DBG1

Expressioni

Gene expression databases

BgeeiENSMUSG00000026170 Expressed in 211 organ(s), highest expression level in left lobe of liver
GenevisibleiQ9DBG1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027356

Structurei

3D structure databases

ProteinModelPortaliQ9DBG1
SMRiQ9DBG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 400Sterol-bindingSequence analysisAdd BLAST15

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140779
HOGENOMiHOG000253961
HOVERGENiHBG106909
InParanoidiQ9DBG1
KOiK00488
OMAiVIDDFMT
OrthoDBiEOG091G0MI3
PhylomeDBiQ9DBG1
TreeFamiTF105094

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBG1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAWSRTRLR WTLLDPRVVG RGLCPQGARA KATIPAALQA QESTEGPGTG
60 70 80 90 100
QDRPRLRSPA ELPGTGTLQF LFQLFLQGYV LHLPDLQVLN KTKYGPMWTT
110 120 130 140 150
SFGTYTNVNL ASAPLLEQVM RQEGKYPIRD HMDQWKDHRD HKGLTYGIFI
160 170 180 190 200
AQGEQWYHLR QALKQRLLKP DEAALYTDAL NEVISDFITR LDQVRAESES
210 220 230 240 250
GDQVPDMAHL LYHLALEAIT YILFEKRIGC LKPSIPEDTA AFIRSVAIMF
260 270 280 290 300
QNSVYITFLP KWTRPLLPFW KRYLNGWDNI FSFGKKLIDE KVQELKAQLQ
310 320 330 340 350
ETGPDGVRVS GYLHFLLTNE LLSTQETIGT FPELLLAGVD TTSNTLTWAL
360 370 380 390 400
YHLSKSPEIQ EALHKEVTGV VPFGKVPQHK DFAHMPLLKA VIKETLRLYP
410 420 430 440 450
VVPTNSRIIT EKETEINGFL FPKNTQFVLC HYVVSRDPSV FPEPNSFQPH
460 470 480 490 500
RWLRKKEADN PGILHPFGSV PFGYGVRSCL GRRIAELEMQ LMLSRLVQKY
510 520 530
EIALAPGMGE VKTVSRIVLV PSKKVRLHFL QRQ
Length:533
Mass (Da):60,720
Last modified:June 1, 2001 - v1
Checksum:iA7F808CA505EF4E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004977 mRNA Translation: BAB23713.1
BC055028 mRNA Translation: AAH55028.1
CCDSiCCDS15054.1
RefSeqiNP_077226.2, NM_024264.5
UniGeneiMm.85083

Genome annotation databases

EnsembliENSMUST00000027356; ENSMUSP00000027356; ENSMUSG00000026170
GeneIDi104086
KEGGimmu:104086
UCSCiuc007bna.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004977 mRNA Translation: BAB23713.1
BC055028 mRNA Translation: AAH55028.1
CCDSiCCDS15054.1
RefSeqiNP_077226.2, NM_024264.5
UniGeneiMm.85083

3D structure databases

ProteinModelPortaliQ9DBG1
SMRiQ9DBG1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027356

PTM databases

iPTMnetiQ9DBG1
PhosphoSitePlusiQ9DBG1
SwissPalmiQ9DBG1

Proteomic databases

MaxQBiQ9DBG1
PaxDbiQ9DBG1
PeptideAtlasiQ9DBG1
PRIDEiQ9DBG1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027356; ENSMUSP00000027356; ENSMUSG00000026170
GeneIDi104086
KEGGimmu:104086
UCSCiuc007bna.1 mouse

Organism-specific databases

CTDi1593
MGIiMGI:88594 Cyp27a1

Phylogenomic databases

eggNOGiKOG0159 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140779
HOGENOMiHOG000253961
HOVERGENiHBG106909
InParanoidiQ9DBG1
KOiK00488
OMAiVIDDFMT
OrthoDBiEOG091G0MI3
PhylomeDBiQ9DBG1
TreeFamiTF105094

Enzyme and pathway databases

UniPathwayi
UPA00955

ReactomeiR-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-MMU-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-MMU-211976 Endogenous sterols

Miscellaneous databases

PROiPR:Q9DBG1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026170 Expressed in 211 organ(s), highest expression level in left lobe of liver
GenevisibleiQ9DBG1 MM

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP27A_MOUSE
AccessioniPrimary (citable) accession number: Q9DBG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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