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Protein

Dual specificity protein phosphatase 6

Gene

Dusp6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inactivates MAP kinases. Has a specificity for the ERK family (By similarity). Plays an important role in alleviating acute postoperative pain (PubMed:24155322, PubMed:28405172). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (PubMed:24155322). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (PubMed:28405172).By similarity2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei293Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-112409 RAF-independent MAPK1/3 activation
R-MMU-202670 ERKs are inactivated
R-MMU-5675221 Negative regulation of MAPK pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 6 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 3
Short name:
MAP kinase phosphatase 3
Short name:
MKP-3
Gene namesi
Name:Dusp6
Synonyms:Mkp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914853 Dusp6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice exhibit a persistent state of mechanical allodynia following plantar incision (PubMed:24155322, PubMed:28405172). This allodynia phenotype is concurrent with long-lasting spinal phosphorylation of MAPK1/3 and MAP kinase p38 (PubMed:24155322). Tissue at the local incision site also shows prolonged expression of phosphorylated MAPK1/3 which persists through to post-operative day 12, although levels of phosphorylated MAP kinase p38 are normal (PubMed:28405172).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948051 – 381Dual specificity protein phosphatase 6Add BLAST381

Proteomic databases

MaxQBiQ9DBB1
PaxDbiQ9DBB1
PRIDEiQ9DBB1

PTM databases

iPTMnetiQ9DBB1
PhosphoSitePlusiQ9DBB1

Expressioni

Inductioni

Up-regulated in local wound tissue 5-7 days after surgical incision.1 Publication

Gene expression databases

BgeeiENSMUSG00000019960 Expressed in 379 organ(s), highest expression level in forelimb bud
CleanExiMM_DUSP6
ExpressionAtlasiQ9DBB1 baseline and differential
GenevisibleiQ9DBB1 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk1P630852EBI-7812384,EBI-397697

Protein-protein interaction databases

BioGridi212304, 1 interactor
CORUMiQ9DBB1
IntActiQ9DBB1, 1 interactor
MINTiQ9DBB1
STRINGi10090.ENSMUSP00000020118

Structurei

3D structure databases

ProteinModelPortaliQ9DBB1
SMRiQ9DBB1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 148RhodanesePROSITE-ProRule annotationAdd BLAST119
Domaini206 – 381Tyrosine-protein phosphataseAdd BLAST176

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1717 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOVERGENiHBG007347
InParanoidiQ9DBB1
KOiK21946
OMAiLLVMDCR
OrthoDBiEOG091G0249
PhylomeDBiQ9DBB1
TreeFamiTF105122

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PIRSFiPIRSF000939 MAPK_Ptase, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9DBB1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIDTLRPVPF ASEMAICKTV SWLNEQLELG NERLLLMDCR PQELYESSHI
60 70 80 90 100
ESAINVAIPG IMLRRLQKGN LPVRALFTRC EDRDRFTRRC GTDTVVLYDE
110 120 130 140 150
NSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFALHCETN
160 170 180 190 200
LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS
210 220 230 240 250
NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA
260 270 280 290 300
GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS
310 320 330 340 350
VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS
360 370 380
SPCDNRVPTP QLYFTTPSNQ NVYQVDSLQS T
Length:381
Mass (Da):42,408
Last modified:June 1, 2001 - v1
Checksum:i7EA1FB154F4AD2DA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P715A0A1W2P715_MOUSE
Dual-specificity protein phosphatas...
Dusp6
235Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22W → G in BAB26093 (PubMed:16141072).Curated1
Sequence conflicti34L → F in BAB26093 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005062 mRNA Translation: BAB23786.1
AK009131 mRNA Translation: BAB26093.1
AK088468 mRNA Translation: BAC40372.1
AK088665 mRNA Translation: BAC40489.1
AK159146 mRNA Translation: BAE34853.1
AK159502 mRNA Translation: BAE35135.1
AK159900 mRNA Translation: BAE35465.1
BC003869 mRNA Translation: AAH03869.1
CCDSiCCDS24147.1
RefSeqiNP_080544.1, NM_026268.3
UniGeneiMm.1791

Genome annotation databases

EnsembliENSMUST00000020118; ENSMUSP00000020118; ENSMUSG00000019960
GeneIDi67603
KEGGimmu:67603
UCSCiuc007gxk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005062 mRNA Translation: BAB23786.1
AK009131 mRNA Translation: BAB26093.1
AK088468 mRNA Translation: BAC40372.1
AK088665 mRNA Translation: BAC40489.1
AK159146 mRNA Translation: BAE34853.1
AK159502 mRNA Translation: BAE35135.1
AK159900 mRNA Translation: BAE35465.1
BC003869 mRNA Translation: AAH03869.1
CCDSiCCDS24147.1
RefSeqiNP_080544.1, NM_026268.3
UniGeneiMm.1791

3D structure databases

ProteinModelPortaliQ9DBB1
SMRiQ9DBB1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212304, 1 interactor
CORUMiQ9DBB1
IntActiQ9DBB1, 1 interactor
MINTiQ9DBB1
STRINGi10090.ENSMUSP00000020118

PTM databases

iPTMnetiQ9DBB1
PhosphoSitePlusiQ9DBB1

Proteomic databases

MaxQBiQ9DBB1
PaxDbiQ9DBB1
PRIDEiQ9DBB1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020118; ENSMUSP00000020118; ENSMUSG00000019960
GeneIDi67603
KEGGimmu:67603
UCSCiuc007gxk.2 mouse

Organism-specific databases

CTDi1848
MGIiMGI:1914853 Dusp6

Phylogenomic databases

eggNOGiKOG1717 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOVERGENiHBG007347
InParanoidiQ9DBB1
KOiK21946
OMAiLLVMDCR
OrthoDBiEOG091G0249
PhylomeDBiQ9DBB1
TreeFamiTF105122

Enzyme and pathway databases

ReactomeiR-MMU-112409 RAF-independent MAPK1/3 activation
R-MMU-202670 ERKs are inactivated
R-MMU-5675221 Negative regulation of MAPK pathway

Miscellaneous databases

ChiTaRSiDusp6 mouse
PROiPR:Q9DBB1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019960 Expressed in 379 organ(s), highest expression level in forelimb bud
CleanExiMM_DUSP6
ExpressionAtlasiQ9DBB1 baseline and differential
GenevisibleiQ9DBB1 MM

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PIRSFiPIRSF000939 MAPK_Ptase, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDUS6_MOUSE
AccessioniPrimary (citable) accession number: Q9DBB1
Secondary accession number(s): Q542I5, Q9D7L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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