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Protein

Peroxisomal leader peptide-processing protease

Gene

Tysnd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta-oxidation of fatty acids (By similarity).By similarity1 Publication

Activity regulationi

Inhibited by N-ethylmaleimide (NEM). Not affected by leupeptin or trans-epoxysuccinyl-l-leucylamido-(4-gianidino) butane (E64).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei374Charge relay systemBy similarity1
Active sitei410Charge relay systemBy similarity1
Active sitei483Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-9033241 Peroxisomal protein import
R-MMU-9033500 TYSND1 cleaves peroxisomal proteins

Protein family/group databases

MEROPSiS01.286

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal leader peptide-processing protease (EC:3.4.21.-)
Alternative name(s):
Trypsin domain-containing protein 1
Cleaved into the following 2 chains:
Gene namesi
Name:Tysnd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1919017 Tysnd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002861271 – 568Peroxisomal leader peptide-processing proteaseAdd BLAST568
ChainiPRO_00002861281 – 110Peroxisomal leader peptide-processing protease, 10 kDa formAdd BLAST110
ChainiPRO_0000286129111 – 568Peroxisomal leader peptide-processing protease, 49 kDa formAdd BLAST458

Post-translational modificationi

Self-cleavage gives rise to an N-terminal 10-kDa fragment and C-terminal 49-kDa fragment upon import into the peroxisomes. The full-lengh TYSND1 is the active the proteolytic processing of PTS1- and PTS2-proteins and in self-cleavage, and intermolecular self-cleavage of TYSND1 down-regulates its protease activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110 – 111Cleavage1 Publication2

Proteomic databases

EPDiQ9DBA6
MaxQBiQ9DBA6
PaxDbiQ9DBA6
PeptideAtlasiQ9DBA6
PRIDEiQ9DBA6

PTM databases

iPTMnetiQ9DBA6
PhosphoSitePlusiQ9DBA6
SwissPalmiQ9DBA6

Expressioni

Inductioni

By the proliferator-activated receptor alpha agonist bezafibrate.1 Publication

Gene expression databases

BgeeiENSMUSG00000020087 Expressed in 279 organ(s), highest expression level in pharyngeal arch
CleanExiMM_TYSND1
ExpressionAtlasiQ9DBA6 baseline and differential
GenevisibleiQ9DBA6 MM

Interactioni

Subunit structurei

Homodimer. Forms a heterodimer with the C-terminal cleavage product (49 kDa form). Forms a heterodimer with the N-terminal cleavage product (10 kDa form). Interacts with PEX5. Interacts with LONP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9DBA6, 1 interactor
MINTiQ9DBA6
STRINGi10090.ENSMUSP00000020284

Structurei

3D structure databases

ProteinModelPortaliQ9DBA6
SMRiQ9DBA6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 568Serine proteaseAdd BLAST237

Sequence similaritiesi

Belongs to the peptidase S1B family.Curated

Phylogenomic databases

eggNOGiKOG1320 Eukaryota
COG0265 LUCA
GeneTreeiENSGT00390000014627
HOGENOMiHOG000050236
HOVERGENiHBG101259
InParanoidiQ9DBA6
OMAiCHGGIFT
OrthoDBiEOG091G06CU
PhylomeDBiQ9DBA6
TreeFamiTF331254

Family and domain databases

InterProiView protein in InterPro
IPR017345 Pept_S1A_Tysnd1
IPR009003 Peptidase_S1_PA
IPR039245 TYSND1/DEG15
PANTHERiPTHR21004 PTHR21004, 1 hit
PIRSFiPIRSF037989 Peptidase_S1B_Tysnd1, 1 hit
SUPFAMiSSF50494 SSF50494, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9DBA6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRQWGPSMR VAEQAGCVVS ASRAGQPDAG SWSCSGVILS RNPGLVLCHG
60 70 80 90 100
GIFTPFLRTG SAALTQTGTA FLPGDSCSDD LRLHVQWGPT AASPAGRADQ
110 120 130 140 150
ELPGLCTPQC ASLGLEPGAP SRARARPLQP PRPAQLLLLL SCPAFRSHFA
160 170 180 190 200
RLFGADAVDQ WHFVSSAPDD AVSEEEEEDQ LRALGWFALL RVQRGAAAEE
210 220 230 240 250
RRGPVVTVAP LGAVVKGAPL LACGSPFGAF CPDIFLNTLS RGVLSNAAGP
260 270 280 290 300
LLLTDARCLP GTEGGGVFAA RPAGALVALV AAPLCWKARE WVGLTLLCAA
310 320 330 340 350
APLLQVARWA LARLHPGSAS LSVLLPPPDV STPRGLPLRD LGPPWAAAAV
360 370 380 390 400
LVECGTVWGS GVVVAPRLVV TCRHVAPREA ARVLVHSATP KNVAIWGQVV
410 420 430 440 450
FATQETSPYD IAVVSLEEEL NGVPTPVPAG HFHEGEPVSV VGFGVFGQAC
460 470 480 490 500
GPSVTSGILS AVVRVDGSPV MLQTTCAVHG GSSGGPLFSS GSGDLLGIVA
510 520 530 540 550
SNTRDNNTGA TYPHLNFSIP ITVLQPALKQ YSQTGDLGGL RELDHTTEPV
560
RVVWRLQRPL SEVPRSKL
Length:568
Mass (Da):59,066
Last modified:June 1, 2001 - v1
Checksum:iB7BED91B4DB50E5E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P7A9A0A1W2P7A9_MOUSE
Peroxisomal leader peptide-processi...
Tysnd1
198Annotation score:
A0A1W2P741A0A1W2P741_MOUSE
Peroxisomal leader peptide-processi...
Tysnd1
142Annotation score:
A0A1W2P754A0A1W2P754_MOUSE
Peroxisomal leader peptide-processi...
Tysnd1
102Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005069 mRNA Translation: BAB23793.1
AK154513 mRNA Translation: BAE32642.1
BC119320 mRNA Translation: AAI19321.2
CCDSiCCDS48575.1
RefSeqiNP_001259019.1, NM_001272090.1
NP_001259020.1, NM_001272091.1
NP_001259021.1, NM_001272092.1
NP_082188.1, NM_027912.1
UniGeneiMm.71514

Genome annotation databases

EnsembliENSMUST00000020284; ENSMUSP00000020284; ENSMUSG00000020087
GeneIDi71767
KEGGimmu:71767
UCSCiuc007fgh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005069 mRNA Translation: BAB23793.1
AK154513 mRNA Translation: BAE32642.1
BC119320 mRNA Translation: AAI19321.2
CCDSiCCDS48575.1
RefSeqiNP_001259019.1, NM_001272090.1
NP_001259020.1, NM_001272091.1
NP_001259021.1, NM_001272092.1
NP_082188.1, NM_027912.1
UniGeneiMm.71514

3D structure databases

ProteinModelPortaliQ9DBA6
SMRiQ9DBA6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DBA6, 1 interactor
MINTiQ9DBA6
STRINGi10090.ENSMUSP00000020284

Protein family/group databases

MEROPSiS01.286

PTM databases

iPTMnetiQ9DBA6
PhosphoSitePlusiQ9DBA6
SwissPalmiQ9DBA6

Proteomic databases

EPDiQ9DBA6
MaxQBiQ9DBA6
PaxDbiQ9DBA6
PeptideAtlasiQ9DBA6
PRIDEiQ9DBA6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020284; ENSMUSP00000020284; ENSMUSG00000020087
GeneIDi71767
KEGGimmu:71767
UCSCiuc007fgh.2 mouse

Organism-specific databases

CTDi219743
MGIiMGI:1919017 Tysnd1

Phylogenomic databases

eggNOGiKOG1320 Eukaryota
COG0265 LUCA
GeneTreeiENSGT00390000014627
HOGENOMiHOG000050236
HOVERGENiHBG101259
InParanoidiQ9DBA6
OMAiCHGGIFT
OrthoDBiEOG091G06CU
PhylomeDBiQ9DBA6
TreeFamiTF331254

Enzyme and pathway databases

ReactomeiR-MMU-9033241 Peroxisomal protein import
R-MMU-9033500 TYSND1 cleaves peroxisomal proteins

Miscellaneous databases

PROiPR:Q9DBA6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020087 Expressed in 279 organ(s), highest expression level in pharyngeal arch
CleanExiMM_TYSND1
ExpressionAtlasiQ9DBA6 baseline and differential
GenevisibleiQ9DBA6 MM

Family and domain databases

InterProiView protein in InterPro
IPR017345 Pept_S1A_Tysnd1
IPR009003 Peptidase_S1_PA
IPR039245 TYSND1/DEG15
PANTHERiPTHR21004 PTHR21004, 1 hit
PIRSFiPIRSF037989 Peptidase_S1B_Tysnd1, 1 hit
SUPFAMiSSF50494 SSF50494, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTYSD1_MOUSE
AccessioniPrimary (citable) accession number: Q9DBA6
Secondary accession number(s): Q0VE90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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