UniProtKB - Q9DB73 (NB5R1_MOUSE)
Protein
NADH-cytochrome b5 reductase 1
Gene
Cyb5r1
Organism
Mus musculus (Mouse)
Status
Functioni
NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.By similarity
Catalytic activityi
- EC:1.6.2.2
Cofactori
FADBy similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 136 – 166 | FADBy similarityAdd BLAST | 31 | |
Nucleotide bindingi | 175 – 210 | FADBy similarityAdd BLAST | 36 |
GO - Molecular functioni
- cytochrome-b5 reductase activity, acting on NAD(P)H Source: UniProtKB-EC
- FAD binding Source: GO_Central
GO - Biological processi
- sterol biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | FAD, Flavoprotein, NAD |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-cytochrome b5 reductase 1 (EC:1.6.2.2)Short name: b5R.1 Alternative name(s): NAD(P)H:quinone oxidoreductase type 3 polypeptide A2 |
Gene namesi | Name:Cyb5r1 Synonyms:Nqo3a2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919267, Cyb5r1 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Mitochondrion
- mitochondrion Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000287546 | 1 – 305 | NADH-cytochrome b5 reductase 1Add BLAST | 305 |
Proteomic databases
EPDi | Q9DB73 |
jPOSTi | Q9DB73 |
PaxDbi | Q9DB73 |
PeptideAtlasi | Q9DB73 |
PRIDEi | Q9DB73 |
PTM databases
iPTMneti | Q9DB73 |
PhosphoSitePlusi | Q9DB73 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026456, Expressed in bone marrow and 300 other tissues |
Genevisiblei | Q9DB73, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 215095, 6 interactors |
STRINGi | 10090.ENSMUSP00000027726 |
Miscellaneous databases
RNActi | Q9DB73, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 156 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 113 |
Sequence similaritiesi
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0534, Eukaryota |
GeneTreei | ENSGT00940000160784 |
HOGENOMi | CLU_1214431_0_0_1 |
InParanoidi | Q9DB73 |
OMAi | HPPEDWA |
OrthoDBi | 1311668at2759 |
PhylomeDBi | Q9DB73 |
TreeFami | TF314333 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9DB73-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGIQPSPVLL ASLGVGLLTL LGLALGTYLV RRSRRPQVTL QDPDEKYLLR
60 70 80 90 100
LLDKTTVSHN TRRFRFALPT AHHILGLPVG KHVYLSARID GSLVIRPYTP
110 120 130 140 150
VTSDEDQGYV DLVIKVYLKG VHPKFPEGGK MSQYLDSLKI GDMVEFRGPS
160 170 180 190 200
GLLSYAGKGN FNIQPNKKSP PELRVAKKLG MIAGGTGITP MLQLIRAILK
210 220 230 240 250
VPEDPTQCFL LFANQTERDI ILREDLEELQ AQYPNRFKLW FTLDSPPEDW
260 270 280 290 300
TYSKGFVTAD MIQEHLPAPA EDVLLLLCGP PPMVQLACHP NLDKLGYSQK
MRFTY
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UZG6 | G3UZG6_MOUSE | Cytochrome-b5 reductase | Cyb5r1 | 176 | Annotation score: | ||
G3UZ57 | G3UZ57_MOUSE | NADH-cytochrome b5 reductase 1 | Cyb5r1 | 100 | Annotation score: | ||
G3UXV8 | G3UXV8_MOUSE | NADH-cytochrome b5 reductase 1 | Cyb5r1 | 80 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025557 | 159 – 228 | GNFNI…EDLEE → ESACVHGCTWAKSPEATMLY GKADFTLWRLPEETYASPSF TKDFPLAKDQKRKLFSSSHG DDPSTVPCLW in isoform 2. 1 PublicationAdd BLAST | 70 | |
Alternative sequenceiVSP_025558 | 229 – 305 | Missing in isoform 2. 1 PublicationAdd BLAST | 77 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK005159 mRNA Translation: BAB23850.1 AK159663 mRNA Translation: BAE35270.1 AK167178 mRNA Translation: BAE39313.1 BC016266 mRNA Translation: AAH16266.1 BC024618 mRNA Translation: AAH24618.1 |
CCDSi | CCDS15308.1 [Q9DB73-1] |
RefSeqi | NP_082333.1, NM_028057.2 [Q9DB73-1] |
Genome annotation databases
Ensembli | ENSMUST00000027726; ENSMUSP00000027726; ENSMUSG00000026456 [Q9DB73-1] ENSMUST00000154237; ENSMUSP00000133385; ENSMUSG00000026456 [Q9DB73-2] |
GeneIDi | 72017 |
KEGGi | mmu:72017 |
UCSCi | uc007cru.1, mouse [Q9DB73-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK005159 mRNA Translation: BAB23850.1 AK159663 mRNA Translation: BAE35270.1 AK167178 mRNA Translation: BAE39313.1 BC016266 mRNA Translation: AAH16266.1 BC024618 mRNA Translation: AAH24618.1 |
CCDSi | CCDS15308.1 [Q9DB73-1] |
RefSeqi | NP_082333.1, NM_028057.2 [Q9DB73-1] |
3D structure databases
SMRi | Q9DB73 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 215095, 6 interactors |
STRINGi | 10090.ENSMUSP00000027726 |
PTM databases
iPTMneti | Q9DB73 |
PhosphoSitePlusi | Q9DB73 |
Proteomic databases
EPDi | Q9DB73 |
jPOSTi | Q9DB73 |
PaxDbi | Q9DB73 |
PeptideAtlasi | Q9DB73 |
PRIDEi | Q9DB73 |
Protocols and materials databases
Antibodypediai | 2536, 246 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027726; ENSMUSP00000027726; ENSMUSG00000026456 [Q9DB73-1] ENSMUST00000154237; ENSMUSP00000133385; ENSMUSG00000026456 [Q9DB73-2] |
GeneIDi | 72017 |
KEGGi | mmu:72017 |
UCSCi | uc007cru.1, mouse [Q9DB73-1] |
Organism-specific databases
CTDi | 51706 |
MGIi | MGI:1919267, Cyb5r1 |
Phylogenomic databases
eggNOGi | KOG0534, Eukaryota |
GeneTreei | ENSGT00940000160784 |
HOGENOMi | CLU_1214431_0_0_1 |
InParanoidi | Q9DB73 |
OMAi | HPPEDWA |
OrthoDBi | 1311668at2759 |
PhylomeDBi | Q9DB73 |
TreeFami | TF314333 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-1237044, Erythrocytes take up carbon dioxide and release oxygen |
Miscellaneous databases
BioGRID-ORCSi | 72017, 0 hits in 17 CRISPR screens |
ChiTaRSi | Cyb5r1, mouse |
PROi | PR:Q9DB73 |
RNActi | Q9DB73, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026456, Expressed in bone marrow and 300 other tissues |
Genevisiblei | Q9DB73, MM |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NB5R1_MOUSE | |
Accessioni | Q9DB73Primary (citable) accession number: Q9DB73 Secondary accession number(s): Q91W81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 15, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families