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Protein

WD repeat domain-containing protein 83

Gene

Wdr83

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular scaffold protein for various multimeric protein complexes. Involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). Also acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. May also be involved in pre-mRNA splicing.By similarity1 Publication

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-5674135 MAP2K and MAPK activation

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain-containing protein 83
Alternative name(s):
Mitogen-activated protein kinase organizer 1
Short name:
MAPK organizer 1
Gene namesi
Name:Wdr83
Synonyms:Morg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1915086 Wdr83

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002352641 – 315WD repeat domain-containing protein 83Add BLAST315

Proteomic databases

EPDiQ9DAJ4
PaxDbiQ9DAJ4
PeptideAtlasiQ9DAJ4
PRIDEiQ9DAJ4

PTM databases

PhosphoSitePlusiQ9DAJ4

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000005150 Expressed in 251 organ(s), highest expression level in bone marrow
CleanExiMM_1500041N16RIK
ExpressionAtlasiQ9DAJ4 baseline and differential
GenevisibleiQ9DAJ4 MM

Interactioni

Subunit structurei

Interacts with EGLN3/PHD3. Identified in the spliceosome C complex (By similarity). Interacts with ERK signaling proteins MAP2K1/MEK1, MAP2K2/MEK2, LAMTOR3, ARAF/Raf-1, MAPK1/ERK2 and MAPK3/ERK1.By similarity1 Publication

Protein-protein interaction databases

BioGridi212467, 1 interactor
STRINGi10090.ENSMUSP00000091048

Structurei

3D structure databases

ProteinModelPortaliQ9DAJ4
SMRiQ9DAJ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 62WD 1Add BLAST40
Repeati65 – 104WD 2Add BLAST40
Repeati107 – 146WD 3Add BLAST40
Repeati151 – 188WD 4Add BLAST38
Repeati190 – 228WD 5Add BLAST39
Repeati231 – 272WD 6Add BLAST42
Repeati275 – 313WD 7Add BLAST39

Sequence similaritiesi

Belongs to the WD repeat MORG1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0316 Eukaryota
ENOG410XRZ0 LUCA
GeneTreeiENSGT00930000150962
HOGENOMiHOG000091642
HOVERGENiHBG105288
InParanoidiQ9DAJ4
KOiK13124
OMAiMCWDIRT
OrthoDBiEOG091G0G62
PhylomeDBiQ9DAJ4
TreeFamiTF314828

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9DAJ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAFPEPKPRA PELPQKRMKT LDCSQGAVRA VRFNVDGNYC LTCGSDKTLK
60 70 80 90 100
LWNPLRGTLL RTYSGHGYEV LDAAGSFDNS HLCSGGGDKT VVLWDVATGQ
110 120 130 140 150
VVRKFRGHAG KVNTVQFNEE ATVILSGSID SSVRCWDCRS RKPEPVQTLD
160 170 180 190 200
EARDGISSVK VSDHEILAGS VDGRVRRYDL RMGQVSSDYV GSPITCTCFS
210 220 230 240 250
RDGQCTLISS LDSTLRLLDK DTGELLGEYV GHKNQQYKLD CCLSERDTHV
260 270 280 290 300
VSCSEDGKVF FWDLVEGALA LALPVGSNVV QSLAYHPTEP CLLTAMGGSI
310
QYWREETYEA EGGAG
Length:315
Mass (Da):34,444
Last modified:June 1, 2001 - v1
Checksum:i678024513FAA1192
GO
Isoform 2 (identifier: Q9DAJ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-130: KVNTVQFNEEATVILSGSID → VSTSEKTLVNVCIVEIHISP
     131-315: Missing.

Note: No experimental confirmation available.
Show »
Length:130
Mass (Da):14,146
Checksum:i6B26ACE130CC3884
GO
Isoform 3 (identifier: Q9DAJ4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-138: KVNTVQFNEEATVILSGSIDSSVRCWDC → VSTSEKTLVNVCIVEQAEAYGPDTKDAD
     139-315: Missing.

Note: No experimental confirmation available.
Show »
Length:138
Mass (Da):14,961
Checksum:i9BB54A57CF6DD736
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RGR4D6RGR4_MOUSE
WD repeat domain-containing protein...
Wdr83
262Annotation score:
F7C259F7C259_MOUSE
WD repeat domain-containing protein...
Wdr83
55Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti123V → G in BAB32164 (PubMed:16141072).Curated1
Sequence conflicti150D → N in BAB32164 (PubMed:16141072).Curated1
Sequence conflicti165E → D in BAB32164 (PubMed:16141072).Curated1
Sequence conflicti168A → Q in BAB32164 (PubMed:16141072).Curated1
Sequence conflicti174R → S in BAB32164 (PubMed:16141072).Curated1
Sequence conflicti192S → G in AAH19369 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018417111 – 138KVNTV…RCWDC → VSTSEKTLVNVCIVEQAEAY GPDTKDAD in isoform 3. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_018418111 – 130KVNTV…SGSID → VSTSEKTLVNVCIVEIHISP in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_018419131 – 315Missing in isoform 2. 1 PublicationAdd BLAST185
Alternative sequenceiVSP_018420139 – 315Missing in isoform 3. 1 PublicationAdd BLAST177

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY365434 mRNA Translation: AAQ64635.1
AK005790 mRNA Translation: BAB24241.1
AK020654 mRNA Translation: BAB32164.1
BC005587 mRNA Translation: AAH05587.1
BC019369 mRNA Translation: AAH19369.1
BC094624 mRNA Translation: AAH94624.1
CCDSiCCDS22493.1 [Q9DAJ4-1]
RefSeqiNP_080675.2, NM_026399.2 [Q9DAJ4-1]
UniGeneiMm.334680

Genome annotation databases

EnsembliENSMUST00000093357; ENSMUSP00000091048; ENSMUSG00000005150 [Q9DAJ4-1]
ENSMUST00000149050; ENSMUSP00000121568; ENSMUSG00000005150 [Q9DAJ4-2]
GeneIDi67836
KEGGimmu:67836
UCSCiuc009mph.1 mouse [Q9DAJ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY365434 mRNA Translation: AAQ64635.1
AK005790 mRNA Translation: BAB24241.1
AK020654 mRNA Translation: BAB32164.1
BC005587 mRNA Translation: AAH05587.1
BC019369 mRNA Translation: AAH19369.1
BC094624 mRNA Translation: AAH94624.1
CCDSiCCDS22493.1 [Q9DAJ4-1]
RefSeqiNP_080675.2, NM_026399.2 [Q9DAJ4-1]
UniGeneiMm.334680

3D structure databases

ProteinModelPortaliQ9DAJ4
SMRiQ9DAJ4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212467, 1 interactor
STRINGi10090.ENSMUSP00000091048

PTM databases

PhosphoSitePlusiQ9DAJ4

Proteomic databases

EPDiQ9DAJ4
PaxDbiQ9DAJ4
PeptideAtlasiQ9DAJ4
PRIDEiQ9DAJ4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093357; ENSMUSP00000091048; ENSMUSG00000005150 [Q9DAJ4-1]
ENSMUST00000149050; ENSMUSP00000121568; ENSMUSG00000005150 [Q9DAJ4-2]
GeneIDi67836
KEGGimmu:67836
UCSCiuc009mph.1 mouse [Q9DAJ4-1]

Organism-specific databases

CTDi84292
MGIiMGI:1915086 Wdr83

Phylogenomic databases

eggNOGiKOG0316 Eukaryota
ENOG410XRZ0 LUCA
GeneTreeiENSGT00930000150962
HOGENOMiHOG000091642
HOVERGENiHBG105288
InParanoidiQ9DAJ4
KOiK13124
OMAiMCWDIRT
OrthoDBiEOG091G0G62
PhylomeDBiQ9DAJ4
TreeFamiTF314828

Enzyme and pathway databases

ReactomeiR-MMU-5674135 MAP2K and MAPK activation

Miscellaneous databases

PROiPR:Q9DAJ4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005150 Expressed in 251 organ(s), highest expression level in bone marrow
CleanExiMM_1500041N16RIK
ExpressionAtlasiQ9DAJ4 baseline and differential
GenevisibleiQ9DAJ4 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWDR83_MOUSE
AccessioniPrimary (citable) accession number: Q9DAJ4
Secondary accession number(s): Q505C2
, Q8VEB7, Q99JX9, Q9D235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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