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Protein

Dipeptidase 3

Gene

Dpep3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable metalloprotease which hydrolyzes cystinyl-bis-glycine. May be involved in meiosis.1 Publication

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotation

Activity regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=2.5 mM for cystinyl-bis-glycine1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi94Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi96Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi193Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi193Zinc 2; catalyticPROSITE-ProRule annotation1
    Metal bindingi266Zinc 2; catalyticPROSITE-ProRule annotation1
    Metal bindingi287Zinc 2; catalyticPROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    • male meiotic nuclear division Source: UniProtKB
    • proteolysis Source: MGI

    Keywordsi

    Molecular functionDipeptidase, Hydrolase, Metalloprotease, Protease
    Biological processMeiosis
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiR-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
    R-MMU-5423646 Aflatoxin activation and detoxification

    Protein family/group databases

    MEROPSiM19.011

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidase 3 (EC:3.4.13.19)
    Alternative name(s):
    Membrane-bound dipeptidase 3
    Short name:
    MBD-3
    Protein expressed in male leptotene and zygotene spermatocytes 136
    Short name:
    MLZ-136
    Gene namesi
    Name:Dpep3
    Synonyms:Mbd3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:1919104 Dpep3

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 35Sequence analysisAdd BLAST35
    ChainiPRO_000023161336 – 462Dipeptidase 3Add BLAST427
    PropeptideiPRO_0000231614463 – 493Removed in mature formSequence analysisAdd BLAST31

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi143 ↔ 222PROSITE-ProRule annotation
    Disulfide bondi294 ↔ 326PROSITE-ProRule annotation
    Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi431InterchainPROSITE-ProRule annotation
    Lipidationi462GPI-anchor amidated serineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9DA79
    PeptideAtlasiQ9DA79
    PRIDEiQ9DA79

    PTM databases

    iPTMnetiQ9DA79
    PhosphoSitePlusiQ9DA79

    Expressioni

    Tissue specificityi

    Expressed in testis but not ovary.2 Publications

    Developmental stagei

    expressed in ovary and testis at E15.5.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000031898 Expressed in 58 organ(s), highest expression level in testis
    CleanExiMM_DPEP3
    MM_MBD3
    GenevisibleiQ9DA79 MM

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked (By similarity). Interacts with TEX101; co-localized on the cell surface of spermatocytes, spermatids, and testicular spermatozoa, co-localized only in cytoplasmic droplets of caput and corpus epididymal sperm (PubMed:21724266).PROSITE-ProRule annotation1 Publication

    Protein-protein interaction databases

    IntActiQ9DA79, 1 interactor
    MINTiQ9DA79
    STRINGi10090.ENSMUSP00000034371

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DA79
    SMRiQ9DA79
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG4127 Eukaryota
    COG2355 LUCA
    GeneTreeiENSGT00390000017920
    HOGENOMiHOG000072016
    HOVERGENiHBG002339
    InParanoidiQ9DA79
    KOiK01273
    OMAiTHNCHNR
    OrthoDBiEOG091G09CU
    PhylomeDBiQ9DA79
    TreeFamiTF324523

    Family and domain databases

    CDDicd01301 rDP_like, 1 hit
    InterProiView protein in InterPro
    IPR000180 Dipep_AS
    IPR032466 Metal_Hydrolase
    IPR008257 Pept_M19
    PANTHERiPTHR10443 PTHR10443, 1 hit
    PfamiView protein in Pfam
    PF01244 Peptidase_M19, 1 hit
    SUPFAMiSSF51556 SSF51556, 1 hit
    PROSITEiView protein in PROSITE
    PS00869 RENAL_DIPEPTIDASE_1, 1 hit
    PS51365 RENAL_DIPEPTIDASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DA79-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQPAGLEGPR ALGLRPLGHR LSLLGVLLLV PSLWVTCTLT TPSPSSAPTT
    60 70 80 90 100
    PEASNATTAP GIPNDTATSG VTSDPRLREQ ALALMRDFPL VDGHNDLPLL
    110 120 130 140 150
    LRELFQNQLQ DVNLRNFTRG QTNLDRLRDG LVGAQFWSAY IPCQTQDRDA
    160 170 180 190 200
    VRLALEQIDL IRRMCSAYPE LELVTSADGL NNTQKLACLI GVEGGHSLDT
    210 220 230 240 250
    SLAVLRSFYE LGVRYLTLTF TCSTPWAESA TKFRHHFYTN ISGLTSFGEK
    260 270 280 290 300
    VVEEMNRLGM MIDLSHASDT LVKQTLEVSQ APVIFSHSAA RSVCDNLLNI
    310 320 330 340 350
    PDDILQLLKK NGGIVMVTLS MGVLQCSLFA NVSTVADHFD HIRTVIGSEF
    360 370 380 390 400
    IGIGGSYDGS GRFPQGLEDV STYPVLIEEL LSRGWDEREL QGVLRGNLLR
    410 420 430 440 450
    VFRQVEQVRE KSLGQSPVEV KFPERQQSNT CHSHLLPQPQ EDQHQDTHLK
    460 470 480 490
    VTKLPNILQR ASKAPPHPLP GLMATLTSLA LILWLCCSGH RAV
    Length:493
    Mass (Da):54,247
    Last modified:June 1, 2001 - v1
    Checksum:iB9A0D39BB7CEB619
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF488553 mRNA Translation: AAP84987.1
    AK006085 mRNA Translation: BAB24402.1
    BC051148 mRNA Translation: AAH51148.1
    CCDSiCCDS22624.1
    RefSeqiNP_082236.1, NM_027960.2
    UniGeneiMm.173395

    Genome annotation databases

    EnsembliENSMUST00000034371; ENSMUSP00000034371; ENSMUSG00000031898
    GeneIDi71854
    KEGGimmu:71854
    UCSCiuc009net.1 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF488553 mRNA Translation: AAP84987.1
    AK006085 mRNA Translation: BAB24402.1
    BC051148 mRNA Translation: AAH51148.1
    CCDSiCCDS22624.1
    RefSeqiNP_082236.1, NM_027960.2
    UniGeneiMm.173395

    3D structure databases

    ProteinModelPortaliQ9DA79
    SMRiQ9DA79
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ9DA79, 1 interactor
    MINTiQ9DA79
    STRINGi10090.ENSMUSP00000034371

    Protein family/group databases

    MEROPSiM19.011

    PTM databases

    iPTMnetiQ9DA79
    PhosphoSitePlusiQ9DA79

    Proteomic databases

    PaxDbiQ9DA79
    PeptideAtlasiQ9DA79
    PRIDEiQ9DA79

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000034371; ENSMUSP00000034371; ENSMUSG00000031898
    GeneIDi71854
    KEGGimmu:71854
    UCSCiuc009net.1 mouse

    Organism-specific databases

    CTDi64180
    MGIiMGI:1919104 Dpep3

    Phylogenomic databases

    eggNOGiKOG4127 Eukaryota
    COG2355 LUCA
    GeneTreeiENSGT00390000017920
    HOGENOMiHOG000072016
    HOVERGENiHBG002339
    InParanoidiQ9DA79
    KOiK01273
    OMAiTHNCHNR
    OrthoDBiEOG091G09CU
    PhylomeDBiQ9DA79
    TreeFamiTF324523

    Enzyme and pathway databases

    ReactomeiR-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
    R-MMU-5423646 Aflatoxin activation and detoxification

    Miscellaneous databases

    ChiTaRSiDpep3 mouse
    PROiPR:Q9DA79
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000031898 Expressed in 58 organ(s), highest expression level in testis
    CleanExiMM_DPEP3
    MM_MBD3
    GenevisibleiQ9DA79 MM

    Family and domain databases

    CDDicd01301 rDP_like, 1 hit
    InterProiView protein in InterPro
    IPR000180 Dipep_AS
    IPR032466 Metal_Hydrolase
    IPR008257 Pept_M19
    PANTHERiPTHR10443 PTHR10443, 1 hit
    PfamiView protein in Pfam
    PF01244 Peptidase_M19, 1 hit
    SUPFAMiSSF51556 SSF51556, 1 hit
    PROSITEiView protein in PROSITE
    PS00869 RENAL_DIPEPTIDASE_1, 1 hit
    PS51365 RENAL_DIPEPTIDASE_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDPEP3_MOUSE
    AccessioniPrimary (citable) accession number: Q9DA79
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: June 1, 2001
    Last modified: September 12, 2018
    This is version 120 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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