UniProtKB - Q9D9Z1 (SKAP_MOUSE)
Small kinetochore-associated protein
Knstrn
Functioni
GO - Molecular functioni
- microtubule plus-end binding Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- cell migration Source: MGI
- cellular response to epidermal growth factor stimulus Source: MGI
- chromosome segregation Source: UniProtKB
- microtubule cytoskeleton organization Source: MGI
- mitotic sister chromatid segregation Source: UniProtKB
- regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
- spindle organization Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Small kinetochore-associated proteinShort name: SKAP Alternative name(s): Kinetochore-localized astrin-binding protein Short name: Kinastrin Kinetochore-localized astrin/SPAG5-binding protein TRAF4-associated factor 1 |
Gene namesi | Name:Knstrn Synonyms:D2Ertd750e, Skap, Traf4af1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1289298, Knstrn |
Subcellular locationi
Cytoskeleton
- spindle pole By similarity
Nucleus
- Nucleus By similarity
Other locations
- kinetochore By similarity
Note: Colocalizes with microtubules around centrosomes in prophase and with the mitotic spindle at prometaphase and metaphase. From late prometaphase to anaphase, is highly concentrated on kinetochores. Located at the kinetochore-microtubule interface. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends.By similarity
Cytoskeleton
- centriolar satellite Source: MGI
- microtubule plus-end Source: UniProtKB
- mitotic spindle Source: UniProtKB
- spindle pole Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB-KW
- kinetochore Source: UniProtKB
- ruffle Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274513 | 1 – 312 | Small kinetochore-associated proteinAdd BLAST | 312 |
Proteomic databases
EPDi | Q9D9Z1 |
MaxQBi | Q9D9Z1 |
PaxDbi | Q9D9Z1 |
PRIDEi | Q9D9Z1 |
PTM databases
PhosphoSitePlusi | Q9D9Z1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027331, Expressed in zygote and 210 other tissues |
ExpressionAtlasi | Q9D9Z1, baseline and differential |
Genevisiblei | Q9D9Z1, MM |
Interactioni
Subunit structurei
Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2.
Interacts with SPAG5. Directly binds to microtubules, although at relatively low affinity.
Interacts with CENPE; this interaction greatly favors microtubule-binding.
Interacts with DSN1/MIS13; leading to localization to kinetochores.
Interacts with MAPRE1/EB1; leading to localization to the microtubule plus ends.
Interacts with PRPF19.
By similarityGO - Molecular functioni
- microtubule plus-end binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 206280, 4 interactors |
IntActi | Q9D9Z1, 4 interactors |
MINTi | Q9D9Z1 |
STRINGi | 10090.ENSMUSP00000115860 |
Miscellaneous databases
RNActi | Q9D9Z1, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 156 – 312 | Interaction with SPAG5By similarityAdd BLAST | 157 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 166 – 210 | Sequence analysisAdd BLAST | 45 | |
Coiled coili | 246 – 287 | Sequence analysisAdd BLAST | 42 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S60X, Eukaryota |
GeneTreei | ENSGT00390000010376 |
InParanoidi | Q9D9Z1 |
OrthoDBi | 1460326at2759 |
PhylomeDBi | Q9D9Z1 |
TreeFami | TF336302 |
Family and domain databases
InterProi | View protein in InterPro IPR033373, SKAP |
PANTHERi | PTHR31940, PTHR31940, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAAPEAEAQE TAFRTTGPPT DSEPRPFPPS SRKFPFESAA ADSNEDWAVA
60 70 80 90 100
AEHYLKGSGE NGGWEQPAPG VQPSHPATMA SAKTVCDAQP HSMPSCGLPA
110 120 130 140 150
DTQTRATSKL PVKSKEADLL RHLHPGGPEP DVTKVTKSRR ENGQVKAAET
160 170 180 190 200
ASRRNLRNSY KPFNKQKPEE ELKDKNELLE AVNKQLHQKL TETQGELKDL
210 220 230 240 250
TQKVELLEKF QDNCLALLES KGLNPGQETL ASKQEPTTDH TDSMLLLETL
260 270 280 290 300
KDELKVFNET AKKQMEELQA LKVKLKLKEE ESVQFLEQQT LCKDEASDFT
310
IILEEMEQLL EM
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA3KGG8 | A3KGG8_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 234 | Annotation score: | ||
H3BKK2 | H3BKK2_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 186 | Annotation score: | ||
F7BE71 | F7BE71_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 218 | Annotation score: | ||
H3BKV9 | H3BKV9_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 58 | Annotation score: | ||
A3KGG6 | A3KGG6_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 188 | Annotation score: | ||
H3BJ95 | H3BJ95_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 70 | Annotation score: | ||
F6UVD3 | F6UVD3_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 59 | Annotation score: | ||
H3BJJ3 | H3BJJ3_MOUSE | Small kinetochore-associated protei... | Knstrn D2Ertd750e | 18 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 210 | F → I in BAB24529 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK006328 mRNA Translation: BAB24529.1 BC031709 mRNA Translation: AAH31709.1 Different initiation. |
CCDSi | CCDS16585.1 |
RefSeqi | NP_080688.2, NM_026412.3 |
Genome annotation databases
Ensembli | ENSMUST00000134661; ENSMUSP00000115860; ENSMUSG00000027331 |
GeneIDi | 51944 |
KEGGi | mmu:51944 |
UCSCi | uc008lst.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK006328 mRNA Translation: BAB24529.1 BC031709 mRNA Translation: AAH31709.1 Different initiation. |
CCDSi | CCDS16585.1 |
RefSeqi | NP_080688.2, NM_026412.3 |
3D structure databases
SMRi | Q9D9Z1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206280, 4 interactors |
IntActi | Q9D9Z1, 4 interactors |
MINTi | Q9D9Z1 |
STRINGi | 10090.ENSMUSP00000115860 |
PTM databases
PhosphoSitePlusi | Q9D9Z1 |
Proteomic databases
EPDi | Q9D9Z1 |
MaxQBi | Q9D9Z1 |
PaxDbi | Q9D9Z1 |
PRIDEi | Q9D9Z1 |
Protocols and materials databases
Antibodypediai | 52276, 48 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000134661; ENSMUSP00000115860; ENSMUSG00000027331 |
GeneIDi | 51944 |
KEGGi | mmu:51944 |
UCSCi | uc008lst.2, mouse |
Organism-specific databases
CTDi | 90417 |
MGIi | MGI:1289298, Knstrn |
Phylogenomic databases
eggNOGi | ENOG502S60X, Eukaryota |
GeneTreei | ENSGT00390000010376 |
InParanoidi | Q9D9Z1 |
OrthoDBi | 1460326at2759 |
PhylomeDBi | Q9D9Z1 |
TreeFami | TF336302 |
Miscellaneous databases
BioGRID-ORCSi | 51944, 1 hit in 17 CRISPR screens |
ChiTaRSi | Knstrn, mouse |
PROi | PR:Q9D9Z1 |
RNActi | Q9D9Z1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027331, Expressed in zygote and 210 other tissues |
ExpressionAtlasi | Q9D9Z1, baseline and differential |
Genevisiblei | Q9D9Z1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR033373, SKAP |
PANTHERi | PTHR31940, PTHR31940, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SKAP_MOUSE | |
Accessioni | Q9D9Z1Primary (citable) accession number: Q9D9Z1 Secondary accession number(s): Q8K2D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | February 6, 2007 | |
Last modified: | December 2, 2020 | |
This is version 107 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot