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UniProtKB - Q9D9V3 (ECHD1_MOUSE)
Protein
Ethylmalonyl-CoA decarboxylase
Gene
Echdc1
Organism
Mus musculus (Mouse)
Status
Functioni
Decarboxylates ethylmalonyl-CoA, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading. Also has methylmalonyl-CoA decarboxylase activity at lower level.
1 PublicationCatalytic activityi
- EC:4.1.1.941 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.1.1.941 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
kcat is 10 sec(-1) for (S)-ethylmalonyl-CoA as substrate (in presence of 5 mM ATP). kcat is 1.68 sec(-1) for (S)-methylmalonyl-CoA as substrate (in presence of 5 mM ATP).1 Publication
- KM=0.96 µM for (S)-ethylmalonyl-CoA (in absence of ATP)1 Publication
- KM=6.5 µM for (S)-ethylmalonyl-CoA (in presence of 5 mM ATP)1 Publication
- KM=3.1 µM for (S)-methylmalonyl-CoA (in absence of ATP)1 Publication
- KM=15.1 µM for (S)-methylmalonyl-CoA (in presence of 5 mM ATP)1 Publication
GO - Molecular functioni
- carboxy-lyase activity Source: UniProtKB
- enoyl-CoA hydratase activity Source: GO_Central
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BRENDAi | 4.1.1.94, 3474 |
SABIO-RKi | Q9D9V3 |
Names & Taxonomyi
Protein namesi | Recommended name: Ethylmalonyl-CoA decarboxylase (EC:4.1.1.941 Publication)Alternative name(s): Enoyl-CoA hydratase domain-containing protein 1 Methylmalonyl-CoA decarboxylase Short name: MMCD |
Gene namesi | Name:Echdc1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277169, Echdc1 |
VEuPathDBi | HostDB:ENSMUSG00000019883 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000273247 | 1 – 322 | Ethylmalonyl-CoA decarboxylaseAdd BLAST | 322 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 232 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 232 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 316 | N6-succinyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9D9V3 |
jPOSTi | Q9D9V3 |
MaxQBi | Q9D9V3 |
PaxDbi | Q9D9V3 |
PeptideAtlasi | Q9D9V3 |
PRIDEi | Q9D9V3 |
ProteomicsDBi | 275431 [Q9D9V3-1] 275432 [Q9D9V3-2] |
PTM databases
iPTMneti | Q9D9V3 |
PhosphoSitePlusi | Q9D9V3 |
SwissPalmi | Q9D9V3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000019883, Expressed in skin of back and 289 other tissues |
Genevisiblei | Q9D9V3, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 206721, 12 interactors |
IntActi | Q9D9V3, 1 interactor |
STRINGi | 10090.ENSMUSP00000020034 |
Miscellaneous databases
RNActi | Q9D9V3, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the enoyl-CoA hydratase/isomerase family.Curated
Phylogenomic databases
eggNOGi | KOG1680, Eukaryota |
GeneTreei | ENSGT00880000138038 |
HOGENOMi | CLU_009834_7_6_1 |
InParanoidi | Q9D9V3 |
OMAi | HKQMGLV |
OrthoDBi | 1234730at2759 |
PhylomeDBi | Q9D9V3 |
TreeFami | TF315986 |
Family and domain databases
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9D9V3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRCEVNSKP ISEYFGIPCE NREMAKCLLT SSLSVRTKLL QTGVSLYNTS
60 70 80 90 100
HGFHEEEVKK ILEQFPGGSI DLLKKQNGIG ILTLNNPNKM NAFSGVMMLQ
110 120 130 140 150
LLERVIELEN WTEGKGLIIH GAKNTFCSGS DLNAVKALST PESGVALSMF
160 170 180 190 200
MQNTLTRFMR LPLISVALVQ GWAMGGGAEL TTACDFRLMT EESVIRFVHK
210 220 230 240 250
EMGIVPSWGG TSRLVEIIGS RQALKVLSGT LKLDSKEALN IGLTDEVLQP
260 270 280 290 300
SDETTALEQA QEWLEKFVSG PPQVIRGLKK SVCSARELYI EEALQNERDV
310 320
LETLWGGPAN LEAIAKKGKH TK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CXS3 | E0CXS3_MOUSE | Ethylmalonyl-CoA decarboxylase | Echdc1 | 165 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | R → K in BAC26112 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 4 | C → F in BAE26268 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 22 | R → Q in BAC26112 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 280 | K → Q in BAE26268 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022499 | 1 – 23 | Missing in isoform 2. 1 PublicationAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003965 mRNA Translation: BAB23096.1 AK006444 mRNA Translation: BAB24592.2 AK028775 mRNA Translation: BAC26112.1 AK145162 mRNA Translation: BAE26268.1 AK150932 mRNA Translation: BAE29969.1 AK152285 mRNA Translation: BAE31098.1 AK153454 mRNA Translation: BAE32007.1 AK166589 mRNA Translation: BAE38876.1 AK166634 mRNA Translation: BAE38907.1 AK166660 mRNA Translation: BAE38924.1 BC066183 mRNA Translation: AAH66183.1 |
CCDSi | CCDS23760.1 [Q9D9V3-1] CCDS48528.1 [Q9D9V3-2] |
RefSeqi | NP_001103665.1, NM_001110195.1 [Q9D9V3-2] NP_080131.4, NM_025855.4 [Q9D9V3-1] |
Genome annotation databases
Ensembli | ENSMUST00000020034; ENSMUSP00000020034; ENSMUSG00000019883 [Q9D9V3-1] ENSMUST00000160399; ENSMUSP00000125553; ENSMUSG00000019883 [Q9D9V3-2] |
GeneIDi | 52665 |
KEGGi | mmu:52665 |
UCSCi | uc007esw.2, mouse [Q9D9V3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK003965 mRNA Translation: BAB23096.1 AK006444 mRNA Translation: BAB24592.2 AK028775 mRNA Translation: BAC26112.1 AK145162 mRNA Translation: BAE26268.1 AK150932 mRNA Translation: BAE29969.1 AK152285 mRNA Translation: BAE31098.1 AK153454 mRNA Translation: BAE32007.1 AK166589 mRNA Translation: BAE38876.1 AK166634 mRNA Translation: BAE38907.1 AK166660 mRNA Translation: BAE38924.1 BC066183 mRNA Translation: AAH66183.1 |
CCDSi | CCDS23760.1 [Q9D9V3-1] CCDS48528.1 [Q9D9V3-2] |
RefSeqi | NP_001103665.1, NM_001110195.1 [Q9D9V3-2] NP_080131.4, NM_025855.4 [Q9D9V3-1] |
3D structure databases
AlphaFoldDBi | Q9D9V3 |
SMRi | Q9D9V3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206721, 12 interactors |
IntActi | Q9D9V3, 1 interactor |
STRINGi | 10090.ENSMUSP00000020034 |
PTM databases
iPTMneti | Q9D9V3 |
PhosphoSitePlusi | Q9D9V3 |
SwissPalmi | Q9D9V3 |
Proteomic databases
EPDi | Q9D9V3 |
jPOSTi | Q9D9V3 |
MaxQBi | Q9D9V3 |
PaxDbi | Q9D9V3 |
PeptideAtlasi | Q9D9V3 |
PRIDEi | Q9D9V3 |
ProteomicsDBi | 275431 [Q9D9V3-1] 275432 [Q9D9V3-2] |
Protocols and materials databases
Antibodypediai | 32755, 135 antibodies from 20 providers |
Genome annotation databases
Ensembli | ENSMUST00000020034; ENSMUSP00000020034; ENSMUSG00000019883 [Q9D9V3-1] ENSMUST00000160399; ENSMUSP00000125553; ENSMUSG00000019883 [Q9D9V3-2] |
GeneIDi | 52665 |
KEGGi | mmu:52665 |
UCSCi | uc007esw.2, mouse [Q9D9V3-1] |
Organism-specific databases
CTDi | 55862 |
MGIi | MGI:1277169, Echdc1 |
VEuPathDBi | HostDB:ENSMUSG00000019883 |
Phylogenomic databases
eggNOGi | KOG1680, Eukaryota |
GeneTreei | ENSGT00880000138038 |
HOGENOMi | CLU_009834_7_6_1 |
InParanoidi | Q9D9V3 |
OMAi | HKQMGLV |
OrthoDBi | 1234730at2759 |
PhylomeDBi | Q9D9V3 |
TreeFami | TF315986 |
Enzyme and pathway databases
BRENDAi | 4.1.1.94, 3474 |
SABIO-RKi | Q9D9V3 |
Miscellaneous databases
BioGRID-ORCSi | 52665, 2 hits in 74 CRISPR screens |
ChiTaRSi | Echdc1, mouse |
PROi | PR:Q9D9V3 |
RNActi | Q9D9V3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000019883, Expressed in skin of back and 289 other tissues |
Genevisiblei | Q9D9V3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECHD1_MOUSE | |
Accessioni | Q9D9V3Primary (citable) accession number: Q9D9V3 Secondary accession number(s): Q3U8C0 Q9CTC5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families