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Protein

Equatorin

Gene

Eqtn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.3 Publications

GO - Biological processi

  • acrosomal vesicle exocytosis Source: MGI
  • acrosome assembly Source: MGI
  • endocytosis Source: MGI
  • fusion of sperm to egg plasma membrane involved in single fertilization Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Equatorin
Alternative name(s):
Acrosome formation-associated factor
MN9 antigen
Gene namesi
Name:Eqtn
Synonyms:Afaf, Mn9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1915003 Eqtn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 183LumenalCuratedAdd BLAST163
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Topological domaini205 – 337CytoplasmicCuratedAdd BLAST133

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128T → A: Does not affect MN9 antibody detectability. 1 Publication1
Mutagenesisi129S → A: Does not affect MN9 antibody detectability. 1 Publication1
Mutagenesisi130T → A: Does not affect MN9 antibody detectability. 1 Publication1
Mutagenesisi138T → A: MN9 antibody detectability is lost. 1 Publication1
Mutagenesisi141S → A: Does not affect MN9 antibody detectability. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000028659421 – 337EquatorinAdd BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei336PhosphoserineBy similarity1

Post-translational modificationi

Highly N- and O-glycosylated; contains sialic acid. MN9 epitope is O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9D9V2
PRIDEiQ9D9V2

PTM databases

iPTMnetiQ9D9V2
PhosphoSitePlusiQ9D9V2

Expressioni

Tissue specificityi

Sperm specific, including germ cells (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000028575 Expressed in 46 organ(s), highest expression level in testis
CleanExiMM_4930579C15RIK
GenevisibleiQ9D9V2 MM

Interactioni

Subunit structurei

Interacts with SNAP25.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9D9V2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IX0T Eukaryota
ENOG4111AZ5 LUCA
GeneTreeiENSGT00390000010786
HOGENOMiHOG000033836
HOVERGENiHBG106628
InParanoidiQ9D9V2
KOiK19920
OMAiPSHKNIQ
OrthoDBiEOG091G0OWH
PhylomeDBiQ9D9V2
TreeFamiTF337449

Family and domain databases

InterProiView protein in InterPro
IPR029282 Eqtn/Afaf
PANTHERiPTHR36874 PTHR36874, 1 hit
PfamiView protein in Pfam
PF15339 Afaf, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9D9V2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDFILLIILS GVFLPDIISL QPIVGQEPGV TLSDEEQYYA DEENNTDGNS
60 70 80 90 100
VALHKLEENE MDTPANEKTG NYYKDIKQYV FTTPNIKGSE VSVTATTNLE
110 120 130 140 150
FAVKKNYKAS KPTASGEEEK PSESSRKTST PNIPAFWTIL SKAVNETAVS
160 170 180 190 200
MDDKDQFFQP IPASDLNATN EDKLSELEEI KLKLMLGISL MTLVLLIPLL
210 220 230 240 250
IFCFATLYKL RHLRDKSYES QYSINPELAT LSYFHPTEGV SDTSFSKSAD
260 270 280 290 300
SNSYWVHNSS EMRRSRTRRS KSKPMDFSAG SNQTVLTDES SFLPPEETRF
310 320 330
LLPEEPGKEL IVERGPMQAM NEIDAQLLLN KEGSPSN
Length:337
Mass (Da):37,764
Last modified:May 15, 2007 - v2
Checksum:i3F2103B1EFD10F61
GO
Isoform 2 (identifier: Q9D9V2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-279: Missing.

Show »
Length:296
Mass (Da):33,111
Checksum:iD3019C4A65C48BA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti214R → S in ABC69297 (PubMed:16831425).Curated1
Sequence conflicti214R → S in BAB24593 (PubMed:16141072).Curated1
Sequence conflicti325A → H in ABC69297 (PubMed:16831425).Curated1
Sequence conflicti325A → H in BAB24593 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025111239 – 279Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336137 mRNA Translation: ABC69297.1
AK006446 mRNA Translation: BAB24593.1
AL732611, AL954716 Genomic DNA Translation: CAM14748.1
AL732611, AL954716 Genomic DNA Translation: CAM14749.1
AL954716, AL732611 Genomic DNA Translation: CAM21660.1
AL954716, AL732611 Genomic DNA Translation: CAM21661.1
BC089517 mRNA Translation: AAH89517.1
CCDSiCCDS18362.1 [Q9D9V2-1]
CCDS71422.1 [Q9D9V2-2]
RefSeqiNP_001277552.1, NM_001290623.1 [Q9D9V2-2]
NP_081365.2, NM_027089.4 [Q9D9V2-1]
UniGeneiMm.25097

Genome annotation databases

EnsembliENSMUST00000030309; ENSMUSP00000030309; ENSMUSG00000028575 [Q9D9V2-1]
ENSMUST00000107097; ENSMUSP00000102714; ENSMUSG00000028575 [Q9D9V2-2]
GeneIDi67753
KEGGimmu:67753
UCSCiuc008tsl.3 mouse [Q9D9V2-2]
uc008tsm.2 mouse [Q9D9V2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336137 mRNA Translation: ABC69297.1
AK006446 mRNA Translation: BAB24593.1
AL732611, AL954716 Genomic DNA Translation: CAM14748.1
AL732611, AL954716 Genomic DNA Translation: CAM14749.1
AL954716, AL732611 Genomic DNA Translation: CAM21660.1
AL954716, AL732611 Genomic DNA Translation: CAM21661.1
BC089517 mRNA Translation: AAH89517.1
CCDSiCCDS18362.1 [Q9D9V2-1]
CCDS71422.1 [Q9D9V2-2]
RefSeqiNP_001277552.1, NM_001290623.1 [Q9D9V2-2]
NP_081365.2, NM_027089.4 [Q9D9V2-1]
UniGeneiMm.25097

3D structure databases

ProteinModelPortaliQ9D9V2
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ9D9V2
PhosphoSitePlusiQ9D9V2

Proteomic databases

PaxDbiQ9D9V2
PRIDEiQ9D9V2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030309; ENSMUSP00000030309; ENSMUSG00000028575 [Q9D9V2-1]
ENSMUST00000107097; ENSMUSP00000102714; ENSMUSG00000028575 [Q9D9V2-2]
GeneIDi67753
KEGGimmu:67753
UCSCiuc008tsl.3 mouse [Q9D9V2-2]
uc008tsm.2 mouse [Q9D9V2-1]

Organism-specific databases

CTDi54586
MGIiMGI:1915003 Eqtn

Phylogenomic databases

eggNOGiENOG410IX0T Eukaryota
ENOG4111AZ5 LUCA
GeneTreeiENSGT00390000010786
HOGENOMiHOG000033836
HOVERGENiHBG106628
InParanoidiQ9D9V2
KOiK19920
OMAiPSHKNIQ
OrthoDBiEOG091G0OWH
PhylomeDBiQ9D9V2
TreeFamiTF337449

Miscellaneous databases

PROiPR:Q9D9V2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028575 Expressed in 46 organ(s), highest expression level in testis
CleanExiMM_4930579C15RIK
GenevisibleiQ9D9V2 MM

Family and domain databases

InterProiView protein in InterPro
IPR029282 Eqtn/Afaf
PANTHERiPTHR36874 PTHR36874, 1 hit
PfamiView protein in Pfam
PF15339 Afaf, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEQTN_MOUSE
AccessioniPrimary (citable) accession number: Q9D9V2
Secondary accession number(s): A2AJE0, Q2LCV5, Q5FWB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 7, 2018
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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