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Protein

Izumo sperm-egg fusion protein 1

Gene

Izumo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg (PubMed:15759005, PubMed:24739963, PubMed:27309808). The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion (PubMed:15759005, PubMed:24739963, PubMed:27309808). The ligand-receptor interaction probably does not act as a membrane 'fusogen' (PubMed:15759005, PubMed:24739963, PubMed:27309808).3 Publications

Miscellaneous

Izumo is the name of a Japanese shrine to marriage.1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein binding involved in heterotypic cell-cell adhesion Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • signaling receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processFertilization

Enzyme and pathway databases

ReactomeiR-MMU-1300652 Sperm:Oocyte Membrane Binding

Names & Taxonomyi

Protein namesi
Recommended name:
Izumo sperm-egg fusion protein 1Curated
Alternative name(s):
Oocyte binding/fusion factor1 Publication
Short name:
OBF1 Publication
Sperm-specific protein izumo1 Publication
Gene namesi
Name:Izumo11 PublicationImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1920706 Izumo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 319ExtracellularSequence analysisAdd BLAST298
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 397CytoplasmicSequence analysisAdd BLAST57

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are healthy but the males are sterile (PubMed:15759005). They produce morphologically normal sperm that can bind to and penetrate the zona pellucida but that are incapable of fusing with eggs (PubMed:15759005).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148W → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi154K → A: Decreases adhesion to oocytes. 1 Publication1
Mutagenesisi157H → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi158I → R: Strongly decreases adhesion to oocytes. 1 Publication1
Mutagenesisi160R → A: Abolishes adhesion to oocytes. 1 Publication1
Mutagenesisi163L → A: Decreases adhesion to oocytes. 1 Publication1
Mutagenesisi204N → Q: Almost no change in fusion-facilitating activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004548322 – 397Izumo sperm-egg fusion protein 1Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 1491 PublicationImported
Disulfide bondi25 ↔ 1521 PublicationImported
Disulfide bondi135 ↔ 1591 PublicationImported
Disulfide bondi139 ↔ 1651 PublicationImported
Disulfide bondi182 ↔ 2331 PublicationImported
Glycosylationi204N-linked (GlcNAc...) asparagine2 PublicationsImported1
Modified residuei379PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated. Glycosylation is not essential for fusion and for proper protein trafficking in sperm.1 Publication
Phosphorylated (PubMed:19658160, PubMed:27624483). The cytoplasmic C-terminus is phosphorylated and undergoes phosphorylation changes during epididymal transit (PubMed:27624483).2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9D9J7
PaxDbiQ9D9J7
PRIDEiQ9D9J7

PTM databases

iPTMnetiQ9D9J7
PhosphoSitePlusiQ9D9J7

Expressioni

Tissue specificityi

Sperm-specific (at protein level) (PubMed:15759005, PubMed:19658160, PubMed:20421979). Detectable on sperm surface only after the acrosome reaction (PubMed:15759005).3 Publications

Gene expression databases

BgeeiENSMUSG00000064158 Expressed in 20 organ(s), highest expression level in testis
CleanExiMM_IZUMO1
GenevisibleiQ9D9J7 MM

Interactioni

Subunit structurei

Monomer, homodimer and homooligomer; depending on the context (PubMed:19658160, PubMed:26568141, PubMed:29954238). Interacts with IZUMO1R/JUNO (PubMed:25209248, PubMed:24739963, PubMed:27309808). IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (By similarity). In gamete recognition, IZUMO1R/JUNO first binds to monomeric IZUMO1 (PubMed:26568141, PubMed:29954238). The weak, but specific interaction with IZUMO1R/JUNO induces IZUMO1 homodimerization (PubMed:26568141, PubMed:29954238). The process follows a tight binding phase where IZUMO1 bends the entire structure towards the sperm membrane side through a thiol-disulfide exchange reaction (PubMed:26568141, PubMed:29954238). The molecule no longer binds to IZUMO1R/JUNO and instead binds to a putative second oocyte receptor (PubMed:26568141, PubMed:29954238). Interacts with ACE3 (PubMed:20421979).By similarity7 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033100

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9D9J7
SMRiQ9D9J7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 251Ig-like C2-typeAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 160Important for interaction with IZUMO1R1 PublicationAdd BLAST13

Domaini

The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO (By similarity). Interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation (By similarity).By similarity
The cytoplasmic C-terminus region is not essential for fertilization (PubMed:27624483). It is however required for protein stability (PubMed:27624483).1 Publication

Sequence similaritiesi

Belongs to the Izumo family.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHEV Eukaryota
ENOG410Z8SJ LUCA
GeneTreeiENSGT00390000015014
HOGENOMiHOG000113123
HOVERGENiHBG081813
InParanoidiQ9D9J7
OMAiELFWMLH
OrthoDBiEOG091G0AHL
PhylomeDBiQ9D9J7
TreeFamiTF338356

Family and domain databases

InterProiView protein in InterPro
IPR036179 Ig-like_dom_sf
IPR029389 IZUMO
IPR032699 Izumo-Ig
IPR032700 IZUMO1
PANTHERiPTHR35540 PTHR35540, 1 hit
PfamiView protein in Pfam
PF15005 IZUMO, 1 hit
PF16706 Izumo-Ig, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D9J7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGPHFTLLLA ALANCLCPGR PCIKCDQFVT DALKTFENTY LNDHLPHDIH
60 70 80 90 100
KNVMRMVNHE VSSFGVVTSA EDSYLGAVDE NTLEQATWSF LKDLKRITDS
110 120 130 140 150
DLKGELFIKE LLWMLRHQKD IFNNLARQFQ KEVLCPNKCG VMSQTLIWCL
160 170 180 190 200
KCEKQLHICR KSLDCGERHI EVHRSEDLVL DCLLSWHRAS KGLTDYSFYR
210 220 230 240 250
VWENSSETLI AKGKEPYLTK SMVGPEDAGN YRCVLDTINQ GHATVIRYDV
260 270 280 290 300
TVLPPKHSEE NQPPNIITQE EHETPVHVTP QTPPGQEPES ELYPELHPEL
310 320 330 340 350
YPELIPTVAQ NPEKKMKTRL LILLTLGFVV LVASIIISVL HFRKVSAKLK
360 370 380 390
NASDEVKPTA SGSKSDQSLS QQMGLKKASQ ADFNSDYSGD KSEATEN
Length:397
Mass (Da):44,885
Last modified:June 1, 2001 - v1
Checksum:i70D10D480B78F5C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195681 mRNA Translation: BAD91011.1
AK006830 mRNA Translation: BAB24761.1
CCDSiCCDS21255.1
RefSeqiNP_001018013.1, NM_001018013.1
UniGeneiMm.380445

Genome annotation databases

EnsembliENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158
GeneIDi73456
KEGGimmu:73456
UCSCiuc009gwh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195681 mRNA Translation: BAD91011.1
AK006830 mRNA Translation: BAB24761.1
CCDSiCCDS21255.1
RefSeqiNP_001018013.1, NM_001018013.1
UniGeneiMm.380445

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B5KX-ray2.50A22-257[»]
ProteinModelPortaliQ9D9J7
SMRiQ9D9J7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033100

PTM databases

iPTMnetiQ9D9J7
PhosphoSitePlusiQ9D9J7

Proteomic databases

MaxQBiQ9D9J7
PaxDbiQ9D9J7
PRIDEiQ9D9J7

Protocols and materials databases

DNASUi73456
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033100; ENSMUSP00000033100; ENSMUSG00000064158
GeneIDi73456
KEGGimmu:73456
UCSCiuc009gwh.1 mouse

Organism-specific databases

CTDi284359
MGIiMGI:1920706 Izumo1

Phylogenomic databases

eggNOGiENOG410IHEV Eukaryota
ENOG410Z8SJ LUCA
GeneTreeiENSGT00390000015014
HOGENOMiHOG000113123
HOVERGENiHBG081813
InParanoidiQ9D9J7
OMAiELFWMLH
OrthoDBiEOG091G0AHL
PhylomeDBiQ9D9J7
TreeFamiTF338356

Enzyme and pathway databases

ReactomeiR-MMU-1300652 Sperm:Oocyte Membrane Binding

Miscellaneous databases

PROiPR:Q9D9J7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064158 Expressed in 20 organ(s), highest expression level in testis
CleanExiMM_IZUMO1
GenevisibleiQ9D9J7 MM

Family and domain databases

InterProiView protein in InterPro
IPR036179 Ig-like_dom_sf
IPR029389 IZUMO
IPR032699 Izumo-Ig
IPR032700 IZUMO1
PANTHERiPTHR35540 PTHR35540, 1 hit
PfamiView protein in Pfam
PF15005 IZUMO, 1 hit
PF16706 Izumo-Ig, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIZUM1_MOUSE
AccessioniPrimary (citable) accession number: Q9D9J7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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