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Protein

26S proteasome non-ATPase regulatory subunit 12

Gene

Psmd12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091 Activation of NF-kappaB in B cells
R-MMU-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-MMU-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174113 SCF-beta-TrCP mediated degradation of Emi1
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-187577 SCF(Skp2)-mediated degradation of p27/p21
R-MMU-195253 Degradation of beta-catenin by the destruction complex
R-MMU-202424 Downstream TCR signaling
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2871837 FCERI mediated NF-kB activation
R-MMU-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-MMU-350562 Regulation of ornithine decarboxylase (ODC)
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-4641257 Degradation of AXIN
R-MMU-4641258 Degradation of DVL
R-MMU-5358346 Hedgehog ligand biogenesis
R-MMU-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-5610780 Degradation of GLI1 by the proteasome
R-MMU-5610785 GLI3 is processed to GLI3R by the proteasome
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676590 NIK-->noncanonical NF-kB signaling
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-5689603 UCH proteinases
R-MMU-5689880 Ub-specific processing proteases
R-MMU-6798695 Neutrophil degranulation
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-69229 Ubiquitin-dependent degradation of Cyclin D1
R-MMU-69481 G2/M Checkpoints
R-MMU-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-MMU-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-MMU-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-MMU-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-MMU-8939902 Regulation of RUNX2 expression and activity
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-8948751 Regulation of PTEN stability and activity
R-MMU-9020702 Interleukin-1 signaling
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 12
Alternative name(s):
26S proteasome regulatory subunit RPN5
26S proteasome regulatory subunit p55
Gene namesi
Name:Psmd12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914247 Psmd12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001738622 – 45626S proteasome non-ATPase regulatory subunit 12Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Cross-linki92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki92Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei368N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9D8W5
MaxQBiQ9D8W5
PaxDbiQ9D8W5
PeptideAtlasiQ9D8W5
PRIDEiQ9D8W5

PTM databases

iPTMnetiQ9D8W5
PhosphoSitePlusiQ9D8W5
SwissPalmiQ9D8W5

Expressioni

Gene expression databases

BgeeiENSMUSG00000020720
ExpressionAtlasiQ9D8W5 baseline and differential
GenevisibleiQ9D8W5 MM

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD12, a base containing 6 ATPases and few additional components.By similarity1 Publication

Protein-protein interaction databases

BioGridi211864, 5 interactors
IntActiQ9D8W5, 2 interactors
STRINGi10090.ENSMUSP00000021063

Structurei

3D structure databases

ProteinModelPortaliQ9D8W5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini242 – 420PCIPROSITE-ProRule annotationAdd BLAST179

Sequence similaritiesi

Belongs to the proteasome subunit p55 family.Curated

Phylogenomic databases

eggNOGiKOG1498 Eukaryota
COG5071 LUCA
GeneTreeiENSGT00730000111110
HOGENOMiHOG000194265
HOVERGENiHBG053739
InParanoidiQ9D8W5
KOiK03035
OMAiKQAITKM
OrthoDBiEOG091G06CB
TreeFamiTF105721

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000717 PCI_dom
IPR035297 PSMD12
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10855:SF1 PTHR10855:SF1, 1 hit
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D8W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGGSERAD GRIVKMEVDY SATVDQRLPE CEKLAKEGRL QEVIETLLSL
60 70 80 90 100
EKQTRTASDM VSTSRILVAV VKMCYEAKEW DLLNENIMLL SKRRSQLKQA
110 120 130 140 150
VAKMVQQCCT YVEEITDLPV KLRLIDTLRM VTEGKIYVEI ERARLTKTLA
160 170 180 190 200
TIKEQNGDVK EAASILQELQ VETYGSMEKK ERVEFILEQM RLCLAVKDYI
210 220 230 240 250
RTQIISKKIN TKFFQEENTE NLKLKYYNLM IQLDQHEGSY LSICKHYRAI
260 270 280 290 300
YDTPCIQAES DKWQQALKSV VLYVILAPFD NEQSDLVHRI SSDKKLEEIP
310 320 330 340 350
KYKDLLKLFT TMELMRWSTL VEDYGVELRK GSSETPATDV FSSTEEGEKR
360 370 380 390 400
WKDLKSRVVE HNIRIMAKYY TRITMKRMAQ LLDLSVDESE AFLSNLVVNK
410 420 430 440 450
TIFAKVDRLA GVINFQRPKD PNNLLNDWSQ KLNSLMSLVN KTTHLIAKEE

MIHNLQ
Length:456
Mass (Da):52,895
Last modified:July 27, 2011 - v4
Checksum:i5D2CD4B0D5FC283F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83L → H in BAB25140 (PubMed:16141072).Curated1
Sequence conflicti207K → R in BAB30969 (PubMed:16141072).Curated1
Sequence conflicti214F → S in BAB25140 (PubMed:16141072).Curated1
Sequence conflicti222L → M in BAB25140 (PubMed:16141072).Curated1
Sequence conflicti377R → G in BAB30969 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007619 mRNA Translation: BAB25140.1
AK007680 mRNA Translation: BAB25184.1
AK009969 mRNA Translation: BAB26619.1
AK017843 mRNA Translation: BAB30969.1
AK151242 mRNA Translation: BAE30234.1
AL683815, AL645687 Genomic DNA Translation: CAM20679.1
AL645687, AL683815 Genomic DNA Translation: CAM25445.1
CH466558 Genomic DNA Translation: EDL34331.1
BC004694 mRNA Translation: AAH04694.1
CCDSiCCDS25568.1
RefSeqiNP_080170.1, NM_025894.2
UniGeneiMm.491171

Genome annotation databases

EnsembliENSMUST00000021063; ENSMUSP00000021063; ENSMUSG00000020720
GeneIDi66997
KEGGimmu:66997
UCSCiuc007mar.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPSD12_MOUSE
AccessioniPrimary (citable) accession number: Q9D8W5
Secondary accession number(s): Q9CQT4, Q9CYB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 123 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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