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Protein

Sorting nexin-5

Gene

Snx5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol lipids. Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde transport of lysosomal enzyme receptor IGF2R. May function as link between endosomal transport vesicles and dynactin. Plays a role in the internalization of EGFR after EGF stimulation. Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C and is retromer-independent. Together with PIP5K1C facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation. Involved in E-cadherin sorting and degradation; inhibits PIP5K1C-mediated E-cadherin degradation (By similarity). Plays a role in macropinocytosis (PubMed:18854019).By similarity1 Publication

Caution

The selectivity for particular phosphatidylinositol lipids is under debate. According to one report (PubMed:19553671), the rat protein binds exclusively to phosphatidylinositol 4,5-bisphosphate, while the human protein has been reported (PubMed:15561769) to bind to phosphatidylinositol 3,4-bisphosphate and also to phosphatidylinositol 3-phosphate.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-5
Gene namesi
Name:Snx5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916428 Snx5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002138452 – 404Sorting nexin-5Add BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei193PhosphoserineBy similarity1
Modified residuei275N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D8U8
MaxQBiQ9D8U8
PaxDbiQ9D8U8
PRIDEiQ9D8U8

PTM databases

iPTMnetiQ9D8U8
PhosphoSitePlusiQ9D8U8
SwissPalmiQ9D8U8

Expressioni

Tissue specificityi

Detected in macrophages (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027423 Expressed in 320 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_SNX5
GenevisibleiQ9D8U8 MM

Interactioni

Subunit structurei

Forms heterodimers with BAR domain-containing sorting nexins SNX1 and SNX2; does not homodimerize. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also descibed as vacuolar protein sorting subcomplex (VPS), and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX1, SNX2, VPS26A, VPS29, VPS35, DCTN1, DOCK1, MIB1, PIP5K1C. Interacts with HGS; increased by PIP5K1C kinase activity and by PtdIns(3P) and/or PtdIns(3,4)P2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9D8U8, 1 interactor
STRINGi10090.ENSMUSP00000028909

Structurei

Secondary structure

1404
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9D8U8
SMRiQ9D8U8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 172PXPROSITE-ProRule annotationAdd BLAST148
Domaini202 – 404BARAdd BLAST203

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 46Phosphatidylinositol bisphosphate bindingBy similarity7
Regioni99 – 105Phosphatidylinositol bisphosphate bindingBy similarity7
Regioni113 – 116Phosphatidylinositol bisphosphate bindingBy similarity4
Regioni169 – 261Interaction with DOCK1By similarityAdd BLAST93
Regioni183 – 200Membrane-binding amphipathic helixBy similarityAdd BLAST18

Domaini

The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.By similarity
The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of an amphipatric helix (AH) in the membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiKOG1660 Eukaryota
ENOG410XPZY LUCA
GeneTreeiENSGT00930000150924
HOGENOMiHOG000231691
HOVERGENiHBG000716
InParanoidiQ9D8U8
KOiK17920
OMAiHEDFIWL
OrthoDBiEOG091G08NT
PhylomeDBiQ9D8U8
TreeFamiTF313698

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028654 SNX5
IPR014637 SNX5/SNX6/SNX32
IPR039358 Sorting_nexin-like
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF6 PTHR10555:SF6, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF09325 Vps5, 1 hit
PIRSFiPIRSF036924 Snx5_Snx6, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D8U8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAVPELLEQ QEEDRSKLRS VSVDLNVDPS LQIDIPDALS ERDKVKFTVH
60 70 80 90 100
TKTTLSTFQS PEFSVTRQHE DFVWLHDTLT ETTDYAGLII PPAPTKPDFD
110 120 130 140 150
GPREKMQKLG EGEGSMTKEE FAKMKQELEA EYLAVFKKTV STHEVFLQRL
160 170 180 190 200
SSHPVLSKDR NFHVFLEYDQ DLSVRRKNTK EMFGGFFKSV VKSADEVLFS
210 220 230 240 250
GVKEVDDFFE QEKNFLINYY NRIKDSCAKA DKMTRSHKNV ADDYIHTAAC
260 270 280 290 300
LHSLALEEPT VIKKYLLKVA ELFEKLRKVE GRVSSDEDLK LTELLRYYML
310 320 330 340 350
NIEAAKDLLY RRTKALIDYE NSNKALDKAR LKSKDVKLAE THQQECCQKF
360 370 380 390 400
EQLSESAKEE LINFKRKRVA AFRKNLIEMS ELEIKHARNN VSLLQSCIDL

FKNN
Length:404
Mass (Da):46,797
Last modified:June 1, 2001 - v1
Checksum:iC24D8C78F753FAFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007676 mRNA Translation: BAB25180.1
AK029463 mRNA Translation: BAC26460.1
AK031873 mRNA Translation: BAC27587.1
AK049129 mRNA Translation: BAC33558.1
AK159904 mRNA Translation: BAE35468.1
AK169786 mRNA Translation: BAE41365.1
BC002242 mRNA Translation: AAH02242.1
CCDSiCCDS16815.1
RefSeqiNP_001186117.1, NM_001199188.1
NP_077187.1, NM_024225.5
UniGeneiMm.273379

Genome annotation databases

EnsembliENSMUST00000028909; ENSMUSP00000028909; ENSMUSG00000027423
ENSMUST00000110030; ENSMUSP00000105657; ENSMUSG00000027423
GeneIDi69178
KEGGimmu:69178
UCSCiuc008mqq.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007676 mRNA Translation: BAB25180.1
AK029463 mRNA Translation: BAC26460.1
AK031873 mRNA Translation: BAC27587.1
AK049129 mRNA Translation: BAC33558.1
AK159904 mRNA Translation: BAE35468.1
AK169786 mRNA Translation: BAE41365.1
BC002242 mRNA Translation: AAH02242.1
CCDSiCCDS16815.1
RefSeqiNP_001186117.1, NM_001199188.1
NP_077187.1, NM_024225.5
UniGeneiMm.273379

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TP1X-ray2.31A/B/C/D20-180[»]
ProteinModelPortaliQ9D8U8
SMRiQ9D8U8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D8U8, 1 interactor
STRINGi10090.ENSMUSP00000028909

PTM databases

iPTMnetiQ9D8U8
PhosphoSitePlusiQ9D8U8
SwissPalmiQ9D8U8

Proteomic databases

EPDiQ9D8U8
MaxQBiQ9D8U8
PaxDbiQ9D8U8
PRIDEiQ9D8U8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028909; ENSMUSP00000028909; ENSMUSG00000027423
ENSMUST00000110030; ENSMUSP00000105657; ENSMUSG00000027423
GeneIDi69178
KEGGimmu:69178
UCSCiuc008mqq.3 mouse

Organism-specific databases

CTDi27131
MGIiMGI:1916428 Snx5

Phylogenomic databases

eggNOGiKOG1660 Eukaryota
ENOG410XPZY LUCA
GeneTreeiENSGT00930000150924
HOGENOMiHOG000231691
HOVERGENiHBG000716
InParanoidiQ9D8U8
KOiK17920
OMAiHEDFIWL
OrthoDBiEOG091G08NT
PhylomeDBiQ9D8U8
TreeFamiTF313698

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

PROiPR:Q9D8U8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027423 Expressed in 320 organ(s), highest expression level in stroma of bone marrow
CleanExiMM_SNX5
GenevisibleiQ9D8U8 MM

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028654 SNX5
IPR014637 SNX5/SNX6/SNX32
IPR039358 Sorting_nexin-like
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF6 PTHR10555:SF6, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF09325 Vps5, 1 hit
PIRSFiPIRSF036924 Snx5_Snx6, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNX5_MOUSE
AccessioniPrimary (citable) accession number: Q9D8U8
Secondary accession number(s): Q543N9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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