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Protein

Junctional adhesion molecule C

Gene

Jam3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates cell-cell adhesion. Functions as counter-receptor for JAM2 (PubMed:15372036). Functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (By similarity). Plays a role in angiogenesis (PubMed:15994945). May play a role in the regulation of cell migration (By similarity). Required for normal polarization and acrosome formation in developing spermatids, and for normal male fertility (PubMed:15372036).By similarity2 Publications
Soluble form of JAM-C: Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAngiogenesis, Cell adhesion, Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-216083 Integrin cell surface interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule C
Short name:
JAM-C
Alternative name(s):
JAM-22 Publications
Junctional adhesion molecule 3
Short name:
JAM-3
Cleaved into the following chain:
Soluble form of JAM-C1 Publication
Short name:
sJAM-C1 Publication
Gene namesi
Name:Jam3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933825 Jam3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 241ExtracellularSequence analysisAdd BLAST212
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Topological domaini263 – 310CytoplasmicSequence analysisAdd BLAST48

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Tight junction

Pathology & Biotechi

Disruption phenotypei

Important mortality during the postnatal period; about 40% of the mutant mice survive. Mutant males are infertile; they have strongly reduced testis size and fail to produce mature sperm cells. Developing spermatids fail to become polarized, and do not form acrosomes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001507230 – 310Junctional adhesion molecule CAdd BLAST281
ChainiPRO_000044533731 – ?Soluble form of JAM-C

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 115PROSITE-ProRule annotation
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi160 ↔ 219PROSITE-ProRule annotation
Glycosylationi192N-linked (GlcNAc...) asparagine1 Publication1
Lipidationi264S-palmitoyl cysteineBy similarity1
Lipidationi265S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.By similarity
N-glycosylated.1 Publication
S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ9D8B7
PaxDbiQ9D8B7
PeptideAtlasiQ9D8B7
PRIDEiQ9D8B7

PTM databases

iPTMnetiQ9D8B7
PhosphoSitePlusiQ9D8B7
SwissPalmiQ9D8B7

Expressioni

Tissue specificityi

Colocalizes with Jam2 near the lumen of seminiferous tubulues. Detected at junctional plaques that correspond to cell-cell contacts between spermatids and Sertoli cells (PubMed:15372036, PubMed:28617811). Detected on endothelial cells, in brain vessels and kidney glomeruli (at protein level) (PubMed:11053409, PubMed:11739175). Detected in heart, lung, liver, kidney, testis, thymus, lymph node and Peyer patch (PubMed:11053409, PubMed:11739175). Endothelial cells (PubMed:11739175).4 Publications

Developmental stagei

Detected in diploid pre-meiotic spermatocytes, haploid spermatids and elongated spermatids (PubMed:28617811, PubMed:15372036). Restricted to junctional plaques in the heads of elongated spermatids (at protein level) (PubMed:15372036).2 Publications

Gene expression databases

BgeeiENSMUSG00000031990 Expressed in 273 organ(s), highest expression level in sciatic nerve
CleanExiMM_JAM3
GenevisibleiQ9D8B7 MM

Interactioni

Subunit structurei

Interacts with GORASP2 (PubMed:28617811). Interacts with JAM2 (PubMed:15372036). Interacts with ITGAM (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ9D8B7
DIPiDIP-42154N
IntActiQ9D8B7, 1 interactor
MINTiQ9D8B7
STRINGi10090.ENSMUSP00000034472

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9D8B7
SMRiQ9D8B7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 127Ig-like V-typeAdd BLAST93
Domaini139 – 236Ig-like C2-typeAdd BLAST98

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQI Eukaryota
ENOG41100KX LUCA
GeneTreeiENSGT00730000110678
HOGENOMiHOG000247041
HOVERGENiHBG000518
InParanoidiQ9D8B7
KOiK06785
OMAiQTNDPRI
OrthoDBiEOG091G0DWZ
PhylomeDBiQ9D8B7
TreeFamiTF331459

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D8B7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALSRRLRLR LYARLPDFFL LLLFRGCMIE AVNLKSSNRN PVVHEFESVE
60 70 80 90 100
LSCIITDSQT SDPRIEWKKI QDGQTTYVYF DNKIQGDLAG RTDVFGKTSL
110 120 130 140 150
RIWNVTRSDS AIYRCEVVAL NDRKEVDEIT IELIVQVKPV TPVCRIPAAV
160 170 180 190 200
PVGKTATLQC QESEGYPRPH YSWYRNDVPL PTDSRANPRF QNSSFHVNSE
210 220 230 240 250
TGTLVFNAVH KDDSGQYYCI ASNDAGAARC EGQDMEVYDL NIAGIIGGVL
260 270 280 290 300
VVLIVLAVIT MGICCAYRRG CFISSKQDGE SYKSPGKHDG VNYIRTSEEG
310
DFRHKSSFVI
Length:310
Mass (Da):34,838
Last modified:May 10, 2005 - v2
Checksum:i4B92BCB51D0A4B0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44H → Q in BAB25519 (PubMed:16141072).Curated1
Sequence conflicti172S → N in BAB25519 (PubMed:16141072).Curated1
Sequence conflicti303R → K in BAB22715 (PubMed:16141072).Curated1
Sequence conflicti306 – 307SS → IA in BAB22715 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ300304 mRNA Translation: CAC20704.1
AK008187 mRNA Translation: BAB25519.1
AK003326 mRNA Translation: BAB22715.1
AK013156 mRNA Translation: BAB28683.1
AK017692 mRNA Translation: BAC25526.1
AK032833 mRNA Translation: BAC28049.1
BC024357 mRNA Translation: AAH24357.1
CCDSiCCDS22939.1
RefSeqiNP_075766.1, NM_023277.4
UniGeneiMm.28770

Genome annotation databases

EnsembliENSMUST00000034472; ENSMUSP00000034472; ENSMUSG00000031990
GeneIDi83964
KEGGimmu:83964
UCSCiuc009oqj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ300304 mRNA Translation: CAC20704.1
AK008187 mRNA Translation: BAB25519.1
AK003326 mRNA Translation: BAB22715.1
AK013156 mRNA Translation: BAB28683.1
AK017692 mRNA Translation: BAC25526.1
AK032833 mRNA Translation: BAC28049.1
BC024357 mRNA Translation: AAH24357.1
CCDSiCCDS22939.1
RefSeqiNP_075766.1, NM_023277.4
UniGeneiMm.28770

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GMIX-ray2.71C/D292-310[»]
ProteinModelPortaliQ9D8B7
SMRiQ9D8B7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ9D8B7
DIPiDIP-42154N
IntActiQ9D8B7, 1 interactor
MINTiQ9D8B7
STRINGi10090.ENSMUSP00000034472

PTM databases

iPTMnetiQ9D8B7
PhosphoSitePlusiQ9D8B7
SwissPalmiQ9D8B7

Proteomic databases

MaxQBiQ9D8B7
PaxDbiQ9D8B7
PeptideAtlasiQ9D8B7
PRIDEiQ9D8B7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034472; ENSMUSP00000034472; ENSMUSG00000031990
GeneIDi83964
KEGGimmu:83964
UCSCiuc009oqj.2 mouse

Organism-specific databases

CTDi83700
MGIiMGI:1933825 Jam3

Phylogenomic databases

eggNOGiENOG410IGQI Eukaryota
ENOG41100KX LUCA
GeneTreeiENSGT00730000110678
HOGENOMiHOG000247041
HOVERGENiHBG000518
InParanoidiQ9D8B7
KOiK06785
OMAiQTNDPRI
OrthoDBiEOG091G0DWZ
PhylomeDBiQ9D8B7
TreeFamiTF331459

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-216083 Integrin cell surface interactions

Miscellaneous databases

ChiTaRSiJam3 mouse
PROiPR:Q9D8B7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031990 Expressed in 273 organ(s), highest expression level in sciatic nerve
CleanExiMM_JAM3
GenevisibleiQ9D8B7 MM

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiJAM3_MOUSE
AccessioniPrimary (citable) accession number: Q9D8B7
Secondary accession number(s): Q8BT59, Q9D1M9, Q9EPK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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