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Protein

Calpain-9

Gene

Capn9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi81Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi83Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi88Calcium 1By similarity1
Active sitei97By similarity1
Metal bindingi167Calcium 1By similarity1
Active sitei254By similarity1
Active sitei278By similarity1
Metal bindingi284Calcium 2By similarity1
Metal bindingi291Calcium 2By similarity1
Metal bindingi312Calcium 2; via carbonyl oxygen1
Metal bindingi314Calcium 2By similarity1
Metal bindingi316Calcium 2; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi574 – 5851PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi604 – 6152PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  • calcium ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B29 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.006

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-9 (EC:3.4.22.-)
Alternative name(s):
Digestive tract-specific calpain
New calpain 4
Short name:
nCL-4
Gene namesi
Name:Capn9
Synonyms:Ncl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1920897 Capn9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077231 – 690Calpain-9Add BLAST690

Proteomic databases

MaxQBiQ9D805
PaxDbiQ9D805
PRIDEiQ9D805

PTM databases

iPTMnetiQ9D805
PhosphoSitePlusiQ9D805

Expressioni

Tissue specificityi

Predominantly expressed in stomach and small intestine, although low levels of expression in other organs.

Gene expression databases

BgeeiENSMUSG00000031981 Expressed in 48 organ(s), highest expression level in pyloric antrum
CleanExiMM_CAPN9
ExpressionAtlasiQ9D805 baseline and differential
GenevisibleiQ9D805 MM

Interactioni

Protein-protein interaction databases

CORUMiQ9D805
STRINGi10090.ENSMUSP00000090717

Structurei

3D structure databases

ProteinModelPortaliQ9D805
SMRiQ9D805
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 337Calpain catalyticPROSITE-ProRule annotationAdd BLAST296
Domaini518 – 552EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini561 – 594EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini591 – 626EF-hand 3PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni338 – 521Domain IIIAdd BLAST184
Regioni522 – 690Domain IVAdd BLAST169

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiQ9D805
KOiK08578
OMAiINLTMNI
OrthoDBiEOG091G049E
TreeFamiTF314748

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029542 CAPN9
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF385 PTHR10183:SF385, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9D805-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPYLHRSLRP QPQPVPGDAR TIHSSGQSFE QLRQGCLQSG TLFEDADFPA
60 70 80 90 100
SNVSLFYSER PQVPFVWKRP GEIVEKPEFI LGGATRTDIC QGELGDCWLL
110 120 130 140 150
AAIASLTLNQ KALTRVVPQD QGFGSGYAGI FHFQFWQHSE WLDVVIDDRL
160 170 180 190 200
PTFKDRLVFL HSADHNEFWS ALLEKAYAKL NGSYEALKGG SAIEAMEDFT
210 220 230 240 250
GGVAENFQIR EAPEDFFEIL EKALKRGSLL GCSIDTLNAS ESEARTSLGL
260 270 280 290 300
IKGHAYTVTG LDQVNFHGQR IKLIRVRNPW GQVEWNGPWS DSSPEWRSVD
310 320 330 340 350
LEEQKRLGHT ALDDGEFWMA FKDFKIHFDK VEICNLTPDA LEDSALHRWE
360 370 380 390 400
VTIHQGSWVR GSTAGGCRNF LDTFWTNPQI KLSLTERDEG QEGCTFLAAL
410 420 430 440 450
MQKDRRRLKR FGANMLTIGY AIYQCPDKDG HLSRDFFRYH ASLARSKTFI
460 470 480 490 500
NLREVSERFQ LPPGDYILIP STFEPHQEAD FCLRIFSEKR AVTRDLDENI
510 520 530 540 550
DIDLPELPKP TPQEEETEEE QQFRALFQRV AGEDMEVSAE ELEYVLNAVL
560 570 580 590 600
QKKTALKFKR LSLLSCRNII SLMDTSGNGK LEFEEFRVFW DKLKHWMDLF
610 620 630 640 650
LQFDVDKSGT MSSYELRTAL KAAGFQLGGH LLQLIVLRYA DEDLQLDFDD
660 670 680 690
YLNCLVRLEN ASRVFQSLSV KNKDFIHLNI NEFISLTMNI
Length:690
Mass (Da):78,977
Last modified:July 27, 2011 - v2
Checksum:i188F95D93E113947
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z7U6D3Z7U6_MOUSE
Calpain-9
Capn9
64Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188K → E in BAB25791 (PubMed:16141072).Curated1
Sequence conflicti552K → R in AAB69114 (PubMed:9524069).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008629 mRNA Translation: BAB25791.1
AC126930 Genomic DNA No translation available.
AC135015 Genomic DNA No translation available.
U89513 mRNA Translation: AAB69114.1
CCDSiCCDS40514.1
RefSeqiNP_076198.2, NM_023709.4
UniGeneiMm.6209

Genome annotation databases

EnsembliENSMUST00000093033; ENSMUSP00000090717; ENSMUSG00000031981
GeneIDi73647
KEGGimmu:73647
UCSCiuc009nxf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008629 mRNA Translation: BAB25791.1
AC126930 Genomic DNA No translation available.
AC135015 Genomic DNA No translation available.
U89513 mRNA Translation: AAB69114.1
CCDSiCCDS40514.1
RefSeqiNP_076198.2, NM_023709.4
UniGeneiMm.6209

3D structure databases

ProteinModelPortaliQ9D805
SMRiQ9D805
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiQ9D805
STRINGi10090.ENSMUSP00000090717

Protein family/group databases

MEROPSiC02.006

PTM databases

iPTMnetiQ9D805
PhosphoSitePlusiQ9D805

Proteomic databases

MaxQBiQ9D805
PaxDbiQ9D805
PRIDEiQ9D805

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093033; ENSMUSP00000090717; ENSMUSG00000031981
GeneIDi73647
KEGGimmu:73647
UCSCiuc009nxf.2 mouse

Organism-specific databases

CTDi10753
MGIiMGI:1920897 Capn9

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiQ9D805
KOiK08578
OMAiINLTMNI
OrthoDBiEOG091G049E
TreeFamiTF314748

Enzyme and pathway databases

BRENDAi3.4.22.B29 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix

Miscellaneous databases

PROiPR:Q9D805
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031981 Expressed in 48 organ(s), highest expression level in pyloric antrum
CleanExiMM_CAPN9
ExpressionAtlasiQ9D805 baseline and differential
GenevisibleiQ9D805 MM

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029542 CAPN9
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF385 PTHR10183:SF385, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAN9_MOUSE
AccessioniPrimary (citable) accession number: Q9D805
Secondary accession number(s): E9QNM7, O35919
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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