Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein TMEPAI

Gene

Pmepa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed:20129061). Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionSignal transduction inhibitor
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TMEPAI
Alternative name(s):
NEDD4 WW domain-binding protein 4
Prostate transmembrane protein androgen induced 1
Transmembrane prostate androgen-induced protein
Gene namesi
Name:Pmepa1
Synonyms:N4wbp4, Tmepai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1929600 Pmepa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20LumenalSequence analysisAdd BLAST20
Transmembranei21 – 43HelicalSequence analysisAdd BLAST23
Topological domaini44 – 260CytoplasmicSequence analysisAdd BLAST217

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139Y → A: Alters interaction with NEDD4. 1 Publication1
Mutagenesisi210Y → A: Abolishes interaction with NEDD4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001854431 – 260Protein TMEPAIAdd BLAST260

Proteomic databases

PaxDbiQ9D7R2
PRIDEiQ9D7R2

PTM databases

PhosphoSitePlusiQ9D7R2

Expressioni

Tissue specificityi

Expressed in brain, heart, kidney, bladder, ovary and adrenal gland.1 Publication

Interactioni

Subunit structurei

Interacts with AR. Interacts with LDLRAD4. Interacts (via the SMAD interaction motif) with SMAD2 and SMAD3 (By similarity). Interacts with NEDD4 (via PPxY motifs).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Nedd4P469355EBI-6304097,EBI-773516

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9D7R2, 1 interactor
STRINGi10090.ENSMUSP00000039950

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi136 – 139PPxY motif 14
Motifi164 – 167SMAD interaction motif (SIM)4
Motifi207 – 210PPxY motif 24

Domaini

The PPxY motifs mediate interaction with NEDD4.1 Publication
The SMAD interaction motif is required for interaction with SMAD2 and SMAD3 and the negative regulation of TGF-beta signaling.By similarity

Sequence similaritiesi

Belongs to the PMEPA1 family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYQ Eukaryota
ENOG41126PF LUCA
HOVERGENiHBG059833
InParanoidiQ9D7R2

Family and domain databases

InterProiView protein in InterPro
IPR039122 TMEPAI
PANTHERiPTHR16514:SF5 PTHR16514:SF5, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9D7R2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSPARATAQR SLFPSMEITE LEFVQIVVIV VVMMVMVVMI TCLLSHYKLS
60 70 80 90 100
ARSFISRHSQ ARRRDDGLSS EGCLWPSEST VSGGMPEPQV YAPPRPTDRL
110 120 130 140 150
AVPPFIQRSR FQPTYPYLQH EIALPPTISL SDGEEPPPYQ GPCTLQLRDP
160 170 180 190 200
EQQLELNRES VRAPPNRTIF DSDLIDSTML GGPCPPSSNS GISATCYSSG
210 220 230 240 250
GRMEGPPPTY SEVIGHYPGS SFQHQQSNGP SSLLEGTRLH HSHIAPLENK
260
EKEKQKGHPL
Length:260
Mass (Da):28,716
Last modified:June 1, 2001 - v1
Checksum:i31AD07BD16B0D77D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2APF4A2APF4_MOUSE
Protein TMEPAI
Pmepa1
274Annotation score:
A2APF5A2APF5_MOUSE
Protein TMEPAI
Pmepa1
238Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008976 mRNA Translation: BAB26001.1
AF220208 mRNA Translation: AAG44247.1
UniGeneiMm.73682

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008976 mRNA Translation: BAB26001.1
AF220208 mRNA Translation: AAG44247.1
UniGeneiMm.73682

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D7R2, 1 interactor
STRINGi10090.ENSMUSP00000039950

PTM databases

PhosphoSitePlusiQ9D7R2

Proteomic databases

PaxDbiQ9D7R2
PRIDEiQ9D7R2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1929600 Pmepa1

Phylogenomic databases

eggNOGiENOG410IDYQ Eukaryota
ENOG41126PF LUCA
HOVERGENiHBG059833
InParanoidiQ9D7R2

Miscellaneous databases

ChiTaRSiPmepa1 mouse
PROiPR:Q9D7R2
SOURCEiSearch...

Family and domain databases

InterProiView protein in InterPro
IPR039122 TMEPAI
PANTHERiPTHR16514:SF5 PTHR16514:SF5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPMEPA_MOUSE
AccessioniPrimary (citable) accession number: Q9D7R2
Secondary accession number(s): Q9EQH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again