UniProtKB - Q9D7B1 (DUS2L_MOUSE)
Protein
tRNA-dihydrouridine(20) synthase [NAD(P)+]-like
Gene
Dus2
Organism
Mus musculus (Mouse)
Status
Functioni
Dihydrouridine synthase. Catalyzes the NADPH-dependent synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.By similarity
Catalytic activityi
Cofactori
FMNBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | FMN; via amide nitrogenBy similarity | 1 | |
Binding sitei | 87 | FMNBy similarity | 1 | |
Active sitei | 116 | Proton donorBy similarity | 1 | |
Binding sitei | 155 | FMNBy similarity | 1 | |
Binding sitei | 183 | FMNBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 20 | FMNBy similarity | 3 | |
Nucleotide bindingi | 214 – 216 | FMNBy similarity | 3 | |
Nucleotide bindingi | 242 – 243 | FMNBy similarity | 2 |
GO - Molecular functioni
- double-stranded RNA binding Source: UniProtKB
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: UniProtKB
- NADPH binding Source: UniProtKB
- protein kinase inhibitor activity Source: UniProtKB
- tRNA-dihydrouridine20 synthase activity Source: UniProtKB-EC
- tRNA dihydrouridine synthase activity Source: UniProtKB
GO - Biological processi
- negative regulation of cell death Source: UniProtKB
- tRNA dihydrouridine synthesis Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase, RNA-binding |
Biological process | tRNA processing |
Ligand | Flavoprotein, FMN |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (EC:1.3.1.91By similarity)Alternative name(s): Dihydrouridine synthase 2 tRNA-dihydrouridine synthase 2-like |
Gene namesi | Name:Dus2 Synonyms:Dus2l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913619, Dus2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- Cytoplasm By similarity
Note: Mainly at the endoplasmic reticulum.By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000162158 | 1 – 493 | tRNA-dihydrouridine(20) synthase [NAD(P)+]-likeAdd BLAST | 493 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 445 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9D7B1 |
MaxQBi | Q9D7B1 |
PaxDbi | Q9D7B1 |
PRIDEi | Q9D7B1 |
PTM databases
iPTMneti | Q9D7B1 |
PhosphoSitePlusi | Q9D7B1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031901, Expressed in olfactory epithelium and 251 other tissues |
ExpressionAtlasi | Q9D7B1, baseline and differential |
Genevisiblei | Q9D7B1, MM |
Interactioni
Subunit structurei
Interacts with EPRS1.
Interacts (via DRBM domain) with PRKRA and EIF2AK2/PKR (via DRBM 1 domain).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034375 |
Miscellaneous databases
RNActi | Q9D7B1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9D7B1 |
SMRi | Q9D7B1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9D7B1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 369 – 436 | DRBMAdd BLAST | 68 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 333 | Catalytic domainBy similarityAdd BLAST | 333 | |
Regioni | 367 – 371 | Interaction with tRNABy similarity | 5 | |
Regioni | 420 – 424 | Interaction with tRNABy similarity | 5 |
Domaini
Efficient dihydrouridine synthesis requires the presence of both the catalytic domain and the C-terminal RNA-binding DRBM domain.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2334, Eukaryota |
GeneTreei | ENSGT00550000075019 |
HOGENOMi | CLU_013299_3_0_1 |
InParanoidi | Q9D7B1 |
OMAi | QHPVHCD |
OrthoDBi | 801857at2759 |
PhylomeDBi | Q9D7B1 |
TreeFami | TF106151 |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd02801, DUS_like_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR014720, dsRBD_dom IPR035587, DUS-like_FMN-bd IPR001269, tRNA_hU_synthase IPR018517, tRNA_hU_synthase_CS |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF01207, Dus, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit |
PROSITEi | View protein in PROSITE PS01136, UPF0034, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9D7B1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKML
60 70 80 90 100
QCKRVVNEVL STVDFVAPDD RVVFRTCERE QSRVVFQMGT SDAERALAVA
110 120 130 140 150
RLVENDVAGI DVNMGCPKEY STKGGMGAAL LSDPDKIEKI LSTLVKGTHR
160 170 180 190 200
PVTCKIRILP SLEDTLNLVK RIERTGISAI AVHGRNRDER PQHPVSCEVI
210 220 230 240 250
RAIAETLSIP VIANGGSHDH IQQHVDIEDF RQATAASSVM VARAAMWNPS
260 270 280 290 300
IFLKDGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL
310 320 330 340 350
LHAAQSSQEI CEAFGLGAFY EETIRELDAR RADLLAKTPE AVEEPAEDTS
360 370 380 390 400
GIIKMAIRFD RRAYPPQITP KMCLLEWCRR EKLPQPVYET VQRTIDRMFC
410 420 430 440 450
SVVTVAEQKY QSTLWDKSKK LAEQTAAIVC LRSQGLPEGR LGEESPSLNK
460 470 480 490
RKREAPDQDP GGPRVQEPAL PGEICKKPFV TLDSSEENLL EGC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z4C7 | D3Z4C7_MOUSE | tRNA-dihydrouridine(20) synthase [N... | Dus2 Dus2l | 90 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK009391 mRNA Translation: BAB26260.1 BC058431 mRNA Translation: AAH58431.1 |
CCDSi | CCDS22627.1 |
RefSeqi | NP_001288105.1, NM_001301176.1 NP_079794.1, NM_025518.4 XP_006531341.1, XM_006531278.3 |
Genome annotation databases
Ensembli | ENSMUST00000034375; ENSMUSP00000034375; ENSMUSG00000031901 |
GeneIDi | 66369 |
KEGGi | mmu:66369 |
UCSCi | uc009nfa.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK009391 mRNA Translation: BAB26260.1 BC058431 mRNA Translation: AAH58431.1 |
CCDSi | CCDS22627.1 |
RefSeqi | NP_001288105.1, NM_001301176.1 NP_079794.1, NM_025518.4 XP_006531341.1, XM_006531278.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WHN | NMR | - | A | 350-464 | [»] | |
BMRBi | Q9D7B1 | |||||
SMRi | Q9D7B1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000034375 |
PTM databases
iPTMneti | Q9D7B1 |
PhosphoSitePlusi | Q9D7B1 |
Proteomic databases
EPDi | Q9D7B1 |
MaxQBi | Q9D7B1 |
PaxDbi | Q9D7B1 |
PRIDEi | Q9D7B1 |
Protocols and materials databases
Antibodypediai | 50008, 135 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034375; ENSMUSP00000034375; ENSMUSG00000031901 |
GeneIDi | 66369 |
KEGGi | mmu:66369 |
UCSCi | uc009nfa.2, mouse |
Organism-specific databases
CTDi | 54920 |
MGIi | MGI:1913619, Dus2 |
Phylogenomic databases
eggNOGi | KOG2334, Eukaryota |
GeneTreei | ENSGT00550000075019 |
HOGENOMi | CLU_013299_3_0_1 |
InParanoidi | Q9D7B1 |
OMAi | QHPVHCD |
OrthoDBi | 801857at2759 |
PhylomeDBi | Q9D7B1 |
TreeFami | TF106151 |
Miscellaneous databases
BioGRID-ORCSi | 66369, 0 hits in 17 CRISPR screens |
ChiTaRSi | Dus2, mouse |
EvolutionaryTracei | Q9D7B1 |
PROi | PR:Q9D7B1 |
RNActi | Q9D7B1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031901, Expressed in olfactory epithelium and 251 other tissues |
ExpressionAtlasi | Q9D7B1, baseline and differential |
Genevisiblei | Q9D7B1, MM |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd02801, DUS_like_FMN, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR014720, dsRBD_dom IPR035587, DUS-like_FMN-bd IPR001269, tRNA_hU_synthase IPR018517, tRNA_hU_synthase_CS |
Pfami | View protein in Pfam PF00035, dsrm, 1 hit PF01207, Dus, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 1 hit |
PROSITEi | View protein in PROSITE PS01136, UPF0034, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DUS2L_MOUSE | |
Accessioni | Q9D7B1Primary (citable) accession number: Q9D7B1 Secondary accession number(s): Q6PDX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families