UniProtKB - Q9D6K9 (CERS5_MOUSE)
Protein
Ceramide synthase 5
Gene
Cers5
Organism
Mus musculus (Mouse)
Status
Functioni
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA) as acyl donor (PubMed:12912983, PubMed:15823095, PubMed:16100120, PubMed:17977534, PubMed:17609214, PubMed:26853464). Can use other acyl donors, but with less efficiency (PubMed:15823095).6 Publications
Caution
According to a report, deletion of Cers5 protects from obesity: knockout mice are associated with reduced weight gain and improved systemic health after high fat diet challenge (PubMed:26853464). This result was however not confirmed by another study, which did not observe any protection from diet-induced obesity in knockout mice (PubMed:31150623). Effects observed in the first study might be indirect and caused by a large deletion that affects neighboring genes and/or deletes non-coding RNAs (PubMed:26853464, PubMed:31150623).2 Publications
Some prediction bioinformatics tools predict the presence of a homeobox domain (By similarity). However, the domain is degenerate and residues that are important for DNA-binding are absent (By similarity). Moreover, the protein localizes in the endoplasmic reticulum and not in the nucleus, strongly suggesting that it does not constitute a canonical homeobox domain (PubMed:12912983).Sequence analysis1 Publication
Catalytic activityi
- This reaction proceeds in the forward3 Publications direction.
- 2-hydroxyhexadecanoyl-CoA + sphinganine = CoA + H+ + N-(2-hydroxyhexadecanoyl)-sphinganine2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- EC:2.3.1.241 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Activity regulationi
Inhibited by fumonisin B1.1 Publication
Kineticsi
- KM=1.8 µM for sphinganine1 Publication
- KM=3.6 µM for sphinganine1 Publication
- Vmax=215 pmol/min/mg enzyme with sphinganine as substrate1 Publication
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.5 PublicationsView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
GO - Molecular functioni
- DNA binding Source: InterPro
- N-acyltransferase activity Source: GO_Central
- sphingosine N-acyltransferase activity Source: UniProtKB
GO - Biological processi
- ceramide biosynthetic process Source: UniProtKB
- sphingolipid biosynthetic process Source: MGI
Keywordsi
Molecular function | Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Sphingolipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.24, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
UniPathwayi | UPA00222 |
Chemistry databases
SwissLipidsi | SLP:000000118 |
Names & Taxonomyi
Protein namesi | Recommended name: Ceramide synthase 5Curated (EC:2.3.1.-5 Publications)Short name: CerS5Curated Alternative name(s): LAG1 longevity assurance homolog 51 Publication Sphingosine N-acyltransferase CERS5Curated (EC:2.3.1.241 Publication) Translocating chain-associating membrane protein homolog 41 Publication Short name: TRAM homolog 41 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919199, Cers5 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 43 | Lumenal1 PublicationAdd BLAST | 43 | |
Transmembranei | 44 – 64 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 148 – 168 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 187 – 207 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 214 – 234 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 272 – 292 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 312 – 332 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 333 – 414 | CytoplasmicBy similarityAdd BLAST | 82 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Mice are viable and show no apparent morphological alterations in normal conditions (PubMed:26853464). Decreased palmitoyl (C16:0) ceramide pools (PubMed:26853464).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 129 – 140 | Missing : Abolished ceramide synthase activity. 1 PublicationAdd BLAST | 12 | |
Mutagenesisi | 134 | K → A: Abolished ceramide synthase activity. 1 Publication | 1 | |
Mutagenesisi | 134 | K → R: Does not affect ceramide synthase activity. 1 Publication | 1 | |
Mutagenesisi | 136 – 140 | Missing : Abolished ceramide synthase activity. 1 Publication | 5 | |
Mutagenesisi | 140 | K → A: Abolished ceramide synthase activity. 1 Publication | 1 | |
Mutagenesisi | 140 | K → R: Does not affect ceramide synthase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185515 | 1 – 414 | Ceramide synthase 5Add BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 26 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Post-translational modificationi
Phosphorylated at the C-terminus by CK2.By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q9D6K9 |
PaxDbi | Q9D6K9 |
PeptideAtlasi | Q9D6K9 |
PRIDEi | Q9D6K9 |
PTM databases
GlyGeni | Q9D6K9, 1 site |
iPTMneti | Q9D6K9 |
PhosphoSitePlusi | Q9D6K9 |
SwissPalmi | Q9D6K9 |
Expressioni
Tissue specificityi
Ubiquitously expressed, with highest levels in testis and kidney.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000023021, Expressed in skin of abdomen and 308 other tissues |
ExpressionAtlasi | Q9D6K9, baseline and differential |
Genevisiblei | Q9D6K9, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 215049, 3 interactors |
STRINGi | 10090.ENSMUSP00000023762 |
Miscellaneous databases
RNActi | Q9D6K9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9D6K9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9D6K9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 139 – 340 | TLCPROSITE-ProRule annotationAdd BLAST | 202 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 75 – 136 | Homeobox-likeCuratedAdd BLAST | 62 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 299 – 309 | Last loop motifBy similarityAdd BLAST | 11 |
Domaini
The last loop motif confers selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16:0-CoA) as acyl donor.By similarity
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1607, Eukaryota |
GeneTreei | ENSGT00990000203544 |
HOGENOMi | CLU_028277_1_1_1 |
InParanoidi | Q9D6K9 |
OMAi | SHHLATV |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q9D6K9 |
TreeFami | TF314319 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9D6K9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATAAAETLG LLWGWLWSES FWLPQNVSWA DLEGPGDGYG YPRAQHVLSV
60 70 80 90 100
FPLAVCIFSV RMLFERFIAK PCALRVGIKD SPVNKVEPND TLEKVFVSVT
110 120 130 140 150
KYPDEKRLKG LSKQLDWSVR KIQCWFRHRR NQDKPPTLTK FCESMWRFTY
160 170 180 190 200
YLCIFCYGIR FLWSMPWFWD TRQCWYNYPY QPLSRELYYY YITQLAFYWS
210 220 230 240 250
LMFSQFIDVK RKDFLMMFIH HMIGIMLTTF SYVNNMVRVG ALIFCLHDFA
260 270 280 290 300
DPLLEAAKMA NYARRERLCT TLFVIFGAAF IVSRLAIFPL WILNTTLFES
310 320 330 340 350
WEIIGPYPSW WLFNALLLIL QVLHAIWSYL IVQTASKALS RGKVSKDDRS
360 370 380 390 400
DVESSSEEED ETTHKNNLSG SSSSNGANCM NGYMGGSHLA EEQGTCKATG
410
NLHFRASPHL HSCD
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BJ49 | H3BJ49_MOUSE | Ceramide synthase 5 | Cers5 Lass5 | 241 | Annotation score: | ||
H3BJQ0 | H3BJQ0_MOUSE | Ceramide synthase 5 | Cers5 Lass5 | 251 | Annotation score: | ||
H3BLB3 | H3BLB3_MOUSE | Ceramide synthase 5 | Cers5 Lass5 | 203 | Annotation score: | ||
H3BJH5 | H3BJH5_MOUSE | Ceramide synthase 5 | Cers5 Lass5 | 95 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 82 | P → A in AAK40301 (Ref. 1) Curated | 1 | |
Sequence conflicti | 310 | W → R in BAC35894 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011192 | 345 – 387 | SKDDR…YMGGS → LSQEGLDPGSVPSALSHLLP LFSAWWQLDSSILCMCLRMT AVM in isoform 2. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_011193 | 388 – 414 | Missing in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029533 mRNA Translation: AAK40301.1 AK010241 mRNA Translation: BAB26792.1 AK075694 mRNA Translation: BAC35894.1 AK145589 mRNA Translation: BAE26527.1 AK145762 mRNA Translation: BAE26634.1 BC010670 mRNA Translation: AAH10670.1 BC043059 mRNA Translation: AAH43059.1 BC046797 mRNA Translation: AAH46797.1 |
CCDSi | CCDS27830.1 [Q9D6K9-1] |
RefSeqi | NP_082291.1, NM_028015.2 [Q9D6K9-1] |
Genome annotation databases
Ensembli | ENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021 [Q9D6K9-1] ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021 [Q9D6K9-2] |
GeneIDi | 71949 |
KEGGi | mmu:71949 |
UCSCi | uc007xqf.1, mouse [Q9D6K9-1] uc007xqg.1, mouse [Q9D6K9-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY029533 mRNA Translation: AAK40301.1 AK010241 mRNA Translation: BAB26792.1 AK075694 mRNA Translation: BAC35894.1 AK145589 mRNA Translation: BAE26527.1 AK145762 mRNA Translation: BAE26634.1 BC010670 mRNA Translation: AAH10670.1 BC043059 mRNA Translation: AAH43059.1 BC046797 mRNA Translation: AAH46797.1 |
CCDSi | CCDS27830.1 [Q9D6K9-1] |
RefSeqi | NP_082291.1, NM_028015.2 [Q9D6K9-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CQX | NMR | - | A | 77-135 | [»] | |
5E8N | X-ray | 2.25 | C/F/I/L | 379-387 | [»] | |
5E8O | X-ray | 1.98 | C/F | 379-387 | [»] | |
5E8P | X-ray | 2.00 | C/F | 379-387 | [»] | |
SMRi | Q9D6K9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 215049, 3 interactors |
STRINGi | 10090.ENSMUSP00000023762 |
Chemistry databases
SwissLipidsi | SLP:000000118 |
PTM databases
GlyGeni | Q9D6K9, 1 site |
iPTMneti | Q9D6K9 |
PhosphoSitePlusi | Q9D6K9 |
SwissPalmi | Q9D6K9 |
Proteomic databases
EPDi | Q9D6K9 |
PaxDbi | Q9D6K9 |
PeptideAtlasi | Q9D6K9 |
PRIDEi | Q9D6K9 |
Protocols and materials databases
Antibodypediai | 14183, 201 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023762; ENSMUSP00000023762; ENSMUSG00000023021 [Q9D6K9-1] ENSMUST00000109035; ENSMUSP00000104663; ENSMUSG00000023021 [Q9D6K9-2] |
GeneIDi | 71949 |
KEGGi | mmu:71949 |
UCSCi | uc007xqf.1, mouse [Q9D6K9-1] uc007xqg.1, mouse [Q9D6K9-2] |
Organism-specific databases
CTDi | 91012 |
MGIi | MGI:1919199, Cers5 |
Phylogenomic databases
eggNOGi | KOG1607, Eukaryota |
GeneTreei | ENSGT00990000203544 |
HOGENOMi | CLU_028277_1_1_1 |
InParanoidi | Q9D6K9 |
OMAi | SHHLATV |
OrthoDBi | 987268at2759 |
PhylomeDBi | Q9D6K9 |
TreeFami | TF314319 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
BRENDAi | 2.3.1.24, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 71949, 3 hits in 18 CRISPR screens |
ChiTaRSi | Cers5, mouse |
EvolutionaryTracei | Q9D6K9 |
PROi | PR:Q9D6K9 |
RNActi | Q9D6K9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023021, Expressed in skin of abdomen and 308 other tissues |
ExpressionAtlasi | Q9D6K9, baseline and differential |
Genevisiblei | Q9D6K9, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001356, Homeobox_dom IPR016439, Lag1/Lac1-like IPR006634, TLC-dom |
PANTHERi | PTHR12560, PTHR12560, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF03798, TRAM_LAG1_CLN8, 1 hit |
PIRSFi | PIRSF005225, LAG1_LAC1, 1 hit |
SMARTi | View protein in SMART SM00724, TLC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS50922, TLC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CERS5_MOUSE | |
Accessioni | Q9D6K9Primary (citable) accession number: Q9D6K9 Secondary accession number(s): Q3UL17 Q924Z3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references