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UniProtKB - Q9D6F4 (GBRA4_MOUSE)
Protein
Gamma-aminobutyric acid receptor subunit alpha-4
Gene
Gabra4
Organism
Mus musculus (Mouse)
Status
Functioni
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
GO - Molecular functioni
- GABA-A receptor activity Source: InterPro
- GABA-gated chloride ion channel activity Source: GO_Central
- inhibitory extracellular ligand-gated ion channel activity Source: GO_Central
- neurotransmitter receptor activity Source: GO_Central
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: MGI
GO - Biological processi
- central nervous system development Source: MGI
- chemical synaptic transmission Source: GO_Central
- chloride transmembrane transport Source: GO_Central
- gamma-aminobutyric acid signaling pathway Source: GO_Central
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- regulation of membrane potential Source: GO_Central
- regulation of postsynaptic membrane potential Source: GO_Central
- signal transduction Source: GO_Central
- synaptic transmission, GABAergic Source: GO_Central
Keywordsi
Molecular function | Chloride channel, Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-aminobutyric acid receptor subunit alpha-4Alternative name(s): GABA(A) receptor subunit alpha-4 |
Gene namesi | Name:Gabra4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95616, Gabra4 |
VEuPathDBi | HostDB:ENSMUSG00000029211 |
Subcellular locationi
Plasma Membrane
- dendrite membrane Source: GO_Central
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: MGI
Other locations
- chloride channel complex Source: UniProtKB-KW
- GABA-A receptor complex Source: GO_Central
- GABA-ergic synapse Source: MGI
- neuron projection Source: GO_Central
- postsynapse Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 36 – 258 | ExtracellularCuratedAdd BLAST | 223 | |
Transmembranei | 259 – 280 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 285 – 306 | HelicalCuratedAdd BLAST | 22 | |
Transmembranei | 318 – 340 | HelicalCuratedAdd BLAST | 23 | |
Topological domaini | 341 – 521 | CytoplasmicCuratedAdd BLAST | 181 | |
Transmembranei | 522 – 541 | HelicalCuratedAdd BLAST | 20 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Sequence analysisAdd BLAST | 35 | |
ChainiPRO_0000000442 | 36 – 552 | Gamma-aminobutyric acid receptor subunit alpha-4Add BLAST | 517 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 47 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 144 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 157 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 172 ↔ 186 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q9D6F4 |
PaxDbi | Q9D6F4 |
PRIDEi | Q9D6F4 |
ProteomicsDBi | 273420 |
PTM databases
GlyConnecti | 2323, 6 N-Linked glycans (2 sites) |
GlyGeni | Q9D6F4, 3 sites, 6 N-linked glycans (2 sites) |
iPTMneti | Q9D6F4 |
PhosphoSitePlusi | Q9D6F4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029211, Expressed in lateral geniculate body and 207 other tissues |
Genevisiblei | Q9D6F4, MM |
Interactioni
Subunit structurei
Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
Protein-protein interaction databases
BioGRIDi | 199800, 2 interactors |
ComplexPortali | CPX-2988, GABA-A receptor, alpha-4/beta-2/delta CPX-2989, GABA-A receptor, alpha-4/beta-3/delta |
STRINGi | 10090.ENSMUSP00000031121 |
Miscellaneous databases
RNActi | Q9D6F4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 353 – 480 | DisorderedSequence analysisAdd BLAST | 128 | |
Regioni | 492 – 513 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 377 – 391 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 392 – 406 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 407 – 440 | Polar residuesSequence analysisAdd BLAST | 34 | |
Compositional biasi | 449 – 474 | Polar residuesSequence analysisAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. [View classification]Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159024 |
HOGENOMi | CLU_010920_1_6_1 |
InParanoidi | Q9D6F4 |
OMAi | DANLNMR |
OrthoDBi | 1057372at2759 |
PhylomeDBi | Q9D6F4 |
TreeFami | TF315453 |
Family and domain databases
Gene3Di | 1.20.58.390, 1 hit 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005434, GABBAa4_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01617, GABAARALPHA4 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9D6F4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSVQKVPAI ALCSGVSLAL LHFLCLAACL NESPGQNSKD EKLCPENFTR
60 70 80 90 100
ILDSLLDGYD NRLRPGFGGP VTEVKTDIYV TSFGPVSDVE MEYTMDVFFR
110 120 130 140 150
QTWIDKRLKY DGPIEILRLN NMMVTKVWTP DTFFRNGKKS VSHNMTAPNK
160 170 180 190 200
LFRIMRNGTI LYTMRLTISA ECPMRLVDFP MDGHACPLKF GSYAYPKSEM
210 220 230 240 250
IYTWTKGPEK SVEVPKESSS LVQYDLIGQT VSSETIKSIT GEYIVMTVYF
260 270 280 290 300
HLRRKMGYFM IQTYIPCIMT VILSQVSFWI NKESVPARTV FGITTVLTMT
310 320 330 340 350
TLSISARHSL PKVSYATAMD WFIAVCFAFV FSALIEFAAV NYFTNIQMQK
360 370 380 390 400
AKKKISKPPP EVPAAPVLKE KHTETSLQNT HANLNMRKRT NALVHSESDV
410 420 430 440 450
KSRTEVGNHS SKTSAVQESS EATPKAHLAS SPNPFSRANA AETMSAAARG
460 470 480 490 500
LSSAASPSPH GTLRPASLGS ASTRPAFGSR LGRIKTTVNT TGAAGNVSAT
510 520 530 540 550
PPPPAPPPSG SGTSKIDKYA RILFPVTFGA FNMVYWVVYL SKDTMEKSES
LM
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3USX9 | Q3USX9_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra4 | 578 | Annotation score: | ||
A0A0G2JDR1 | A0A0G2JDR1_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra4 | 94 | Annotation score: | ||
A0A0G2JF83 | A0A0G2JF83_MOUSE | Gamma-aminobutyric acid receptor su... | Gabra4 | 36 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013727 mRNA Translation: BAB28975.1 |
CCDSi | CCDS39107.1 |
RefSeqi | NP_034381.1, NM_010251.2 |
Genome annotation databases
Ensembli | ENSMUST00000031121; ENSMUSP00000031121; ENSMUSG00000029211 |
GeneIDi | 14397 |
KEGGi | mmu:14397 |
UCSCi | uc008xqy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013727 mRNA Translation: BAB28975.1 |
CCDSi | CCDS39107.1 |
RefSeqi | NP_034381.1, NM_010251.2 |
3D structure databases
AlphaFoldDBi | Q9D6F4 |
SMRi | Q9D6F4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199800, 2 interactors |
ComplexPortali | CPX-2988, GABA-A receptor, alpha-4/beta-2/delta CPX-2989, GABA-A receptor, alpha-4/beta-3/delta |
STRINGi | 10090.ENSMUSP00000031121 |
Chemistry databases
ChEMBLi | CHEMBL2094133 |
DrugCentrali | Q9D6F4 |
PTM databases
GlyConnecti | 2323, 6 N-Linked glycans (2 sites) |
GlyGeni | Q9D6F4, 3 sites, 6 N-linked glycans (2 sites) |
iPTMneti | Q9D6F4 |
PhosphoSitePlusi | Q9D6F4 |
Proteomic databases
MaxQBi | Q9D6F4 |
PaxDbi | Q9D6F4 |
PRIDEi | Q9D6F4 |
ProteomicsDBi | 273420 |
Protocols and materials databases
Antibodypediai | 12041, 423 antibodies from 39 providers |
DNASUi | 14397 |
Genome annotation databases
Ensembli | ENSMUST00000031121; ENSMUSP00000031121; ENSMUSG00000029211 |
GeneIDi | 14397 |
KEGGi | mmu:14397 |
UCSCi | uc008xqy.1, mouse |
Organism-specific databases
CTDi | 2557 |
MGIi | MGI:95616, Gabra4 |
VEuPathDBi | HostDB:ENSMUSG00000029211 |
Phylogenomic databases
eggNOGi | KOG3642, Eukaryota |
GeneTreei | ENSGT00940000159024 |
HOGENOMi | CLU_010920_1_6_1 |
InParanoidi | Q9D6F4 |
OMAi | DANLNMR |
OrthoDBi | 1057372at2759 |
PhylomeDBi | Q9D6F4 |
TreeFami | TF315453 |
Enzyme and pathway databases
Reactomei | R-MMU-977443, GABA receptor activation |
Miscellaneous databases
BioGRID-ORCSi | 14397, 3 hits in 72 CRISPR screens |
PROi | PR:Q9D6F4 |
RNActi | Q9D6F4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029211, Expressed in lateral geniculate body and 207 other tissues |
Genevisiblei | Q9D6F4, MM |
Family and domain databases
Gene3Di | 1.20.58.390, 1 hit 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006028, GABAA/Glycine_rcpt IPR001390, GABAAa_rcpt IPR005434, GABBAa4_rcpt IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR01079, GABAARALPHA PR01617, GABAARALPHA4 PR00253, GABAARECEPTR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GBRA4_MOUSE | |
Accessioni | Q9D6F4Primary (citable) accession number: Q9D6F4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 4, 2001 |
Last sequence update: | May 4, 2001 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families