UniProtKB - Q9D666 (SUN1_MOUSE)
SUN domain-containing protein 1
Sun1
Functioni
GO - Molecular functioni
- cytoskeleton-nuclear membrane anchor activity Source: MGI
- lamin binding Source: UniProtKB
- protein-membrane adaptor activity Source: GO_Central
GO - Biological processi
- centrosome localization Source: UniProtKB
- homologous chromosome pairing at meiosis Source: MGI
- meiotic attachment of telomere to nuclear envelope Source: UniProtKB
- nuclear envelope organization Source: MGI
- nuclear matrix anchoring at nuclear membrane Source: MGI
- nucleokinesis involved in cell motility in cerebral cortex radial glia guided migration Source: UniProtKB
- ossification Source: Ensembl
- response to mechanical stimulus Source: Ensembl
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Biological process | Differentiation, Meiosis, Spermatogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: SUN domain-containing protein 1Alternative name(s): Protein unc-84 homolog A Sad1/unc-84 protein-like 1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1924303, Sun1 |
Subcellular locationi
Nucleus
- Nucleus inner membrane 11 Publications; Single-pass type II membrane protein 11 Publications
Note: At oocyte MI stage localized around the spindle, at MII stage localized to the spindle poles. In round spermatids mainly localizes to the posterior pole of the nucleus. This localization is gradually disappearing during spermiogenesis. In elongated spermatids localizes to anterior regions outside the nucleus indicative for isoform 5.2 Publications
Other locations
- acrosome outer membrane 1 Publication
Note: Localized to the anterior pole of spermatids.1 Publication
Nucleus
- integral component of nuclear inner membrane Source: MGI
- meiotic nuclear membrane microtubule tethering complex Source: MGI
- nuclear envelope Source: MGI
- nuclear membrane Source: MGI
- nucleus Source: MGI
Other locations
- acrosomal membrane Source: MGI
- cytoplasm Source: MGI
- integral component of membrane Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- outer acrosomal membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 415 | NuclearAdd BLAST | 415 | |
Transmembranei | 416 – 436 | HelicalAdd BLAST | 21 | |
Topological domaini | 437 – 913 | Perinuclear spaceAdd BLAST | 477 |
Keywords - Cellular componenti
Cytoplasmic vesicle, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218912 | 1 – 913 | SUN domain-containing protein 1Add BLAST | 913 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphoserineBy similarity | 1 | |
Modified residuei | 66 | PhosphoserineCombined sources | 1 | |
Modified residuei | 139 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 759 | Interchain (with KASH domain-containing nesprins)By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
EPDi | Q9D666 |
PaxDbi | Q9D666 |
PeptideAtlasi | Q9D666 |
PRIDEi | Q9D666 |
ProteomicsDBi | 258773 [Q9D666-1] 258774 [Q9D666-2] 258775 [Q9D666-3] 258776 [Q9D666-4] 258777 [Q9D666-5] |
PTM databases
GlyConnecti | 2744, 2 N-Linked glycans (1 site) |
GlyGeni | Q9D666, 1 site |
iPTMneti | Q9D666 |
PhosphoSitePlusi | Q9D666 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000036817, Expressed in spermatocyte and 310 other tissues |
Genevisiblei | Q9D666, MM |
Interactioni
Subunit structurei
Core component of the LINC complex which is composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents is giving rise to specific assemblies. At least SUN1/2-containing core LINC complexes are proposed to be hexameric composed of three protomers of each KASH and SUN domain-containing protein.
Interacts with KASH5 (via the last 22 amino acids); this interaction mediates KASH5 telomere localization by forming a SUN1:KASH5 LINC complex. Isoform 5 is proposed to form a non-nuclear spermatogenesis-specific LINC complex with SYNE3 during sperm head formation.
Interacts with SYNE2 and SYNE1; probably forming respective LINC complexes.
Interacts with A-type lamin with a strong preference for unprocessed A-type lamin compared with the mature protein. Interaction with lamins B1 and C is hardly detectable.
Interacts with NAT10.
Interacts with EMD and TSNAX. Associates with the nuclear pore complex (NPC).
Interacts with CCDC79/TERB1; promoting the accumulation of the LINC complex complexes at the telomere-nuclear envelope attachment sites.
Interacts with IRAG2 (PubMed:29878215).
By similarity11 PublicationsBinary interactionsi
Hide detailsQ9D666
GO - Molecular functioni
- lamin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 218484, 6 interactors |
DIPi | DIP-60732N |
IntActi | Q9D666, 8 interactors |
MINTi | Q9D666 |
STRINGi | 10090.ENSMUSP00000056655 |
Miscellaneous databases
RNActi | Q9D666, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9D666 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 751 – 912 | SUNPROSITE-ProRule annotationAdd BLAST | 162 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 139 | LMNA-bindingBy similarityAdd BLAST | 139 | |
Regioni | 209 – 399 | SYNE2-bindingBy similarityAdd BLAST | 191 | |
Regioni | 310 – 399 | EMD-bindingBy similarityAdd BLAST | 90 | |
Regioni | 703 – 913 | Sufficient for interaction with SYNE1 and SYNE2By similarityAdd BLAST | 211 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 491 – 533 | Sequence analysisAdd BLAST | 43 | |
Coiled coili | 563 – 638 | Sequence analysisAdd BLAST | 76 |
Domaini
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2687, Eukaryota |
GeneTreei | ENSGT00940000155830 |
InParanoidi | Q9D666 |
OMAi | SLQMFHA |
OrthoDBi | 1000585at2759 |
PhylomeDBi | Q9D666 |
TreeFami | TF323915 |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR018539, SUN1 IPR032680, SUN1_N IPR040994, Sun_CC2 IPR012919, SUN_dom |
PANTHERi | PTHR12911:SF23, PTHR12911:SF23, 1 hit |
Pfami | View protein in Pfam PF18580, HTH_SUN2, 1 hit PF09387, MRP, 1 hit PF07738, Sad1_UNC, 1 hit |
PROSITEi | View protein in PROSITE PS51469, SUN, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDFSRLHTYT PPQCVPENTG YTYALSSSYS SDALDFETEH KLEPVFDSPR
60 70 80 90 100
MSRRSLRLVT TASYSSGDSQ AIDSHISTSR ATPAKGRETR TVKQRRSASK
110 120 130 140 150
PAFSINHLSG KGLSSSTSHD SSCSLRSATV LRHPVLDESL IREQTKVDHF
160 170 180 190 200
WGLDDDGDLK GGNKAATQGN GELAAEVASS NGYTCRDCRM LSARTDALTA
210 220 230 240 250
HSAIHGTTSR VYSRDRTLKP RGVSFYLDRT LWLAKSTSSS FASFIVQLFQ
260 270 280 290 300
VVLMKLNFET YKLKGYESRA YESQSYETKS HESEAHLGHC GRMTAGELSR
310 320 330 340 350
VDGESLCDDC KGKKHLEIHT ATHSQLPQPH RVAGAMGRLC IYTGDLLVQA
360 370 380 390 400
LRRTRAAGWS VAEAVWSVLW LAVSAPGKAA SGTFWWLGSG WYQFVTLISW
410 420 430 440 450
LNVFLLTRCL RNICKVFVLL LPLLLLLGAG VSLWGQGNFF SLLPVLNWTA
460 470 480 490 500
MQPTQRVDDS KGMHRPGPLP PSPPPKVDHK ASQWPQESDM GQKVASLSAQ
510 520 530 540 550
CHNHDERLAE LTVLLQKLQI RVDQVDDGRE GLSLWVKNVV GQHLQEMGTI
560 570 580 590 600
EPPDAKTDFM TFHHDHEVRL SNLEDVLRKL TEKSEAIQKE LEETKLKAGS
610 620 630 640 650
RDEEQPLLDR VQHLELELNL LKSQLSDWQH LKTSCEQAGA RIQETVQLMF
660 670 680 690 700
SEDQQGGSLE WLLEKLSSRF VSKDELQVLL HDLELKLLQN ITHHITVTGQ
710 720 730 740 750
APTSEAIVSA VNQAGISGIT EAQAHIIVNN ALKLYSQDKT GMVDFALESG
760 770 780 790 800
GGSILSTRCS ETYETKTALL SLFGVPLWYF SQSPRVVIQP DIYPGNCWAF
810 820 830 840 850
KGSQGYLVVR LSMKIYPTTF TMEHIPKTLS PTGNISSAPK DFAVYGLETE
860 870 880 890 900
YQEEGQPLGR FTYDQEGDSL QMFHTLERPD QAFQIVELRV LSNWGHPEYT
910
CLYRFRVHGE PIQ
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TSM1 | Q3TSM1_MOUSE | SUN domain-containing protein 1 | Sun1 Unc84a | 239 | Annotation score: | ||
D3Z1X3 | D3Z1X3_MOUSE | SUN domain-containing protein 1 | Sun1 | 131 | Annotation score: | ||
D3Z5Q2 | D3Z5Q2_MOUSE | SUN domain-containing protein 1 | Sun1 | 158 | Annotation score: | ||
D3Z3N5 | D3Z3N5_MOUSE | SUN domain-containing protein 1 | Sun1 | 160 | Annotation score: | ||
D3YUW9 | D3YUW9_MOUSE | SUN domain-containing protein 1 | Sun1 | 175 | Annotation score: | ||
F6XYA0 | F6XYA0_MOUSE | SUN domain-containing protein 1 | Sun1 | 256 | Annotation score: | ||
F7AJ01 | F7AJ01_MOUSE | SUN domain-containing protein 1 | Sun1 | 117 | Annotation score: | ||
F6RMJ1 | F6RMJ1_MOUSE | SUN domain-containing protein 1 | Sun1 | 258 | Annotation score: | ||
F7BX07 | F7BX07_MOUSE | SUN domain-containing protein 1 | Sun1 | 197 | Annotation score: | ||
D3Z0L7 | D3Z0L7_MOUSE | SUN domain-containing protein 1 | Sun1 | 53 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 | L → V in AAT90501 (PubMed:17132086).Curated | 1 | |
Sequence conflicti | 108 | L → P in BAE39733 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 439 | F → L in AAT90501 (PubMed:17132086).Curated | 1 | |
Sequence conflicti | 479 | H → N in BAE35723 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 505 | D → G in BAE39733 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 593 | E → A in BAE39733 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 704 | S → F in BAE39733 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 856 – 857 | QP → AA in AAK13526 (PubMed:12036294).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039552 | 221 – 285 | RGVSF…HESEA → P in isoform 4. 1 PublicationAdd BLAST | 65 | |
Alternative sequenceiVSP_058699 | 222 – 377 | Missing in isoform 5. 1 PublicationAdd BLAST | 156 | |
Alternative sequenceiVSP_009346 | 222 – 344 | Missing in isoform 2. 1 PublicationAdd BLAST | 123 | |
Alternative sequenceiVSP_009347 | 308 – 344 | Missing in isoform 3. 1 PublicationAdd BLAST | 37 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY682989 mRNA Translation: AAT90501.1 HQ402597 mRNA Translation: ADP89697.1 AK014585 mRNA Translation: BAB29445.1 AK036187 mRNA Translation: BAC29339.1 AK160281 mRNA Translation: BAE35723.1 AK167686 mRNA Translation: BAE39733.1 AC125065 Genomic DNA No translation available. BC030330 mRNA Translation: AAH30330.1 Different initiation. BC047928 mRNA Translation: AAH47928.1 BC048156 mRNA Translation: AAH48156.1 AF343752 mRNA Translation: AAK13526.1 |
CCDSi | CCDS19804.1 [Q9D666-1] CCDS57395.1 [Q9D666-3] CCDS57396.1 [Q9D666-4] CCDS57397.1 [Q9D666-2] CCDS57398.1 [Q9D666-5] |
RefSeqi | NP_001243044.1, NM_001256115.1 [Q9D666-3] NP_001243045.1, NM_001256116.1 [Q9D666-4] NP_001243046.1, NM_001256117.1 [Q9D666-2] NP_001243047.1, NM_001256118.1 [Q9D666-5] NP_077771.1, NM_024451.2 [Q9D666-1] |
Genome annotation databases
Ensembli | ENSMUST00000058716; ENSMUSP00000056655; ENSMUSG00000036817 [Q9D666-1] ENSMUST00000078690; ENSMUSP00000077756; ENSMUSG00000036817 [Q9D666-4] ENSMUST00000110882; ENSMUSP00000106506; ENSMUSG00000036817 [Q9D666-5] ENSMUST00000110883; ENSMUSP00000106507; ENSMUSG00000036817 [Q9D666-2] ENSMUST00000110884; ENSMUSP00000106508; ENSMUSG00000036817 [Q9D666-3] |
GeneIDi | 77053 |
KEGGi | mmu:77053 |
UCSCi | uc009agb.2, mouse [Q9D666-1] uc009agc.2, mouse [Q9D666-3] uc009agd.2, mouse [Q9D666-2] uc009age.2, mouse [Q9D666-4] uc012efr.2, mouse |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY682989 mRNA Translation: AAT90501.1 HQ402597 mRNA Translation: ADP89697.1 AK014585 mRNA Translation: BAB29445.1 AK036187 mRNA Translation: BAC29339.1 AK160281 mRNA Translation: BAE35723.1 AK167686 mRNA Translation: BAE39733.1 AC125065 Genomic DNA No translation available. BC030330 mRNA Translation: AAH30330.1 Different initiation. BC047928 mRNA Translation: AAH47928.1 BC048156 mRNA Translation: AAH48156.1 AF343752 mRNA Translation: AAK13526.1 |
CCDSi | CCDS19804.1 [Q9D666-1] CCDS57395.1 [Q9D666-3] CCDS57396.1 [Q9D666-4] CCDS57397.1 [Q9D666-2] CCDS57398.1 [Q9D666-5] |
RefSeqi | NP_001243044.1, NM_001256115.1 [Q9D666-3] NP_001243045.1, NM_001256116.1 [Q9D666-4] NP_001243046.1, NM_001256117.1 [Q9D666-2] NP_001243047.1, NM_001256118.1 [Q9D666-5] NP_077771.1, NM_024451.2 [Q9D666-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YWZ | X-ray | 2.20 | A | 672-913 | [»] | |
SMRi | Q9D666 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 218484, 6 interactors |
DIPi | DIP-60732N |
IntActi | Q9D666, 8 interactors |
MINTi | Q9D666 |
STRINGi | 10090.ENSMUSP00000056655 |
PTM databases
GlyConnecti | 2744, 2 N-Linked glycans (1 site) |
GlyGeni | Q9D666, 1 site |
iPTMneti | Q9D666 |
PhosphoSitePlusi | Q9D666 |
Proteomic databases
EPDi | Q9D666 |
PaxDbi | Q9D666 |
PeptideAtlasi | Q9D666 |
PRIDEi | Q9D666 |
ProteomicsDBi | 258773 [Q9D666-1] 258774 [Q9D666-2] 258775 [Q9D666-3] 258776 [Q9D666-4] 258777 [Q9D666-5] |
Protocols and materials databases
Antibodypediai | 1893, 106 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000058716; ENSMUSP00000056655; ENSMUSG00000036817 [Q9D666-1] ENSMUST00000078690; ENSMUSP00000077756; ENSMUSG00000036817 [Q9D666-4] ENSMUST00000110882; ENSMUSP00000106506; ENSMUSG00000036817 [Q9D666-5] ENSMUST00000110883; ENSMUSP00000106507; ENSMUSG00000036817 [Q9D666-2] ENSMUST00000110884; ENSMUSP00000106508; ENSMUSG00000036817 [Q9D666-3] |
GeneIDi | 77053 |
KEGGi | mmu:77053 |
UCSCi | uc009agb.2, mouse [Q9D666-1] uc009agc.2, mouse [Q9D666-3] uc009agd.2, mouse [Q9D666-2] uc009age.2, mouse [Q9D666-4] uc012efr.2, mouse |
Organism-specific databases
CTDi | 23353 |
MGIi | MGI:1924303, Sun1 |
Phylogenomic databases
eggNOGi | KOG2687, Eukaryota |
GeneTreei | ENSGT00940000155830 |
InParanoidi | Q9D666 |
OMAi | SLQMFHA |
OrthoDBi | 1000585at2759 |
PhylomeDBi | Q9D666 |
TreeFami | TF323915 |
Miscellaneous databases
BioGRID-ORCSi | 77053, 1 hit in 52 CRISPR screens |
ChiTaRSi | Sun1, mouse |
PROi | PR:Q9D666 |
RNActi | Q9D666, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036817, Expressed in spermatocyte and 310 other tissues |
Genevisiblei | Q9D666, MM |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR018539, SUN1 IPR032680, SUN1_N IPR040994, Sun_CC2 IPR012919, SUN_dom |
PANTHERi | PTHR12911:SF23, PTHR12911:SF23, 1 hit |
Pfami | View protein in Pfam PF18580, HTH_SUN2, 1 hit PF09387, MRP, 1 hit PF07738, Sad1_UNC, 1 hit |
PROSITEi | View protein in PROSITE PS51469, SUN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUN1_MOUSE | |
Accessioni | Q9D666Primary (citable) accession number: Q9D666 Secondary accession number(s): D3Z0V9 Q99P23 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | February 2, 2004 | |
Last modified: | April 7, 2021 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references