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Protein

Cullin-5

Gene

Cul5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  • cerebral cortex radially oriented cell migration Source: MGI
  • protein ubiquitination Source: MGI
  • radial glia guided migration of Purkinje cell Source: MGI
  • regulation of cytosolic calcium ion concentration Source: MGI
  • ubiquitin-dependent protein catabolic process Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi6.3.2.19 3474
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-5
Short name:
CUL-5
Gene namesi
Name:Cul5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1922967 Cul5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197981 – 780Cullin-5Add BLAST780

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei210PhosphothreonineBy similarity1
Cross-linki724Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9D5V5
MaxQBiQ9D5V5
PaxDbiQ9D5V5
PRIDEiQ9D5V5

PTM databases

iPTMnetiQ9D5V5
PhosphoSitePlusiQ9D5V5

Expressioni

Gene expression databases

CleanExiMM_CUL5

Interactioni

Subunit structurei

Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL5, Elongin BC (ELOB and ELOC), RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component. Component of the probable ECS(LRRC41) complex with the substrate recognition component LRRC41. Component of the probable ECS(SOCS1) complex with the substrate recognition component SOCS1. Component of the probable ECS(WSB1) complex with the substrate recognition subunit WSB1. Component of the probable ECS(SOCS3) complex with the substrate recognition component SOCS3. Component of the probable ECS(SPSB1) complex with the substrate recognition component SPSB1. Component of the probable ECS(SPSB2) complex with the substrate recognition component SPSB2. Component of the probable ECS(SPSB4) complex with the substrate recognition component SPSB4. Component of the probable ECS(RAB40C) complex with the substrate recognition subunit RAB40C. May also form complexes containing CUL5, elongin BC complex (ELOB and ELOC), RBX1 and ELOA. May also form complexes containing CUL5, Elongin BC (ELOB and ELOC), RBX1 and VHL. Interacts with RNF7/RBX2, LRRC41, SOCS3, SPSB1, SPSB2, SPSB4 and RAB40C. Interacts with ASB1, ASB2, ASB6, ASB7 and ASB12. Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217689, 21 interactors
CORUMiQ9D5V5
IntActiQ9D5V5, 2 interactors
STRINGi10090.ENSMUSP00000034529

Structurei

Secondary structure

1780
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9D5V5
SMRiQ9D5V5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D5V5

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2285 Eukaryota
COG5647 LUCA
HOGENOMiHOG000007610
HOVERGENiHBG099672
InParanoidiQ9D5V5
KOiK10612
PhylomeDBiQ9D5V5

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9D5V5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATSNLLKNK GSLQFEDKWD FMHPIVLKLL RQESVTKQQW FDLFSDVHAV
60 70 80 90 100
CLWDDKGSSK IHQALKEDIL EFIKQAQARV LSHQDDTALL KAYIVEWRKF
110 120 130 140 150
FTQCDILPKP FCQLEVTLLG KQSSNKKSNM EDSIVRKLML DTWNESIFSN
160 170 180 190 200
IKNRLQDSAM KLVHAERLGE AFDSQLVIGV RESYVNLCSN PEDKLQIYRD
210 220 230 240 250
NFEKAYLDST ERFYRTQAPS YLQQNGVQNY MKYADAKLKE EEKRALRYLE
260 270 280 290 300
TRRECNSVEA LMECCVNALV TSFKETILAE CQGMIKRNET EKLHLMFSLM
310 320 330 340 350
DKVPNGIEPM LKDLEEHIIS AGLADMVAAA ETITTDSEKY VEQLLTLFNR
360 370 380 390 400
FSKLVKEAFQ DDPRFLTARD KAYKAVVNDA TIFKLELPLK QKGVGLKTQP
410 420 430 440 450
ESKCPELLAN YCDMLLRKTP LSKKLTSEEI EAKLKEVLLV LKYVQNKDVF
460 470 480 490 500
MRYHKAHLTR RLILDISADS EIEENMVEWL REVGMPADYV NKLARMFQDI
510 520 530 540 550
KVSEDLNQAF KEMHKNNKLA LPADSVNIKI LNAGAWSRSS EKVFVSLPTE
560 570 580 590 600
LEDLIPEVEE FYKKNHSGRK LHWHHLMSNG IITFKNEVGQ YDLEVTTFQL
610 620 630 640 650
AVLFAWNQRP REKISFENLK LATELPDAEL RRTLWSLVAF PKLKRQVLLY
660 670 680 690 700
DPQVNSPKDF TEGTLFSVNQ DFSLIKNAKV QKRGKINLIG RLQLTTERMR
710 720 730 740 750
EEENEGIVQL RILRTQEAII QIMKMRKKIS NAQLQTELVE ILKNMFLPQK
760 770 780
KMIKEQMEWL IEHRYIRRDE ADINTFIYMA
Length:780
Mass (Da):90,974
Last modified:January 23, 2007 - v3
Checksum:iC6EE7A1AF1038C0D
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PV12E9PV12_MOUSE
Cullin-5
Cul5
828Annotation score:
E9Q6Z0E9Q6Z0_MOUSE
Cullin-5
Cul5
651Annotation score:
G3X914G3X914_MOUSE
Cullin-5
Cul5 mCG_5637
855Annotation score:
F8VQI6F8VQI6_MOUSE
Cullin-5
Cul5
59Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti573W → C in BAC27621 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014894 mRNA Translation: BAB29609.1
AK030306 mRNA Translation: BAC26889.1
AK031955 mRNA Translation: BAC27621.1
AK046030 mRNA Translation: BAC32575.1
AK080305 mRNA Translation: BAC37872.1
BC075710 mRNA Translation: AAH75710.1
RefSeqiNP_001155090.1, NM_001161618.1
NP_082083.2, NM_027807.3
UniGeneiMm.218910
Mm.491606

Genome annotation databases

GeneIDi75717
KEGGimmu:75717
UCSCiuc009pmj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014894 mRNA Translation: BAB29609.1
AK030306 mRNA Translation: BAC26889.1
AK031955 mRNA Translation: BAC27621.1
AK046030 mRNA Translation: BAC32575.1
AK080305 mRNA Translation: BAC37872.1
BC075710 mRNA Translation: AAH75710.1
RefSeqiNP_001155090.1, NM_001161618.1
NP_082083.2, NM_027807.3
UniGeneiMm.218910
Mm.491606

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZKX-ray2.05A1-384[»]
ProteinModelPortaliQ9D5V5
SMRiQ9D5V5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217689, 21 interactors
CORUMiQ9D5V5
IntActiQ9D5V5, 2 interactors
STRINGi10090.ENSMUSP00000034529

PTM databases

iPTMnetiQ9D5V5
PhosphoSitePlusiQ9D5V5

Proteomic databases

EPDiQ9D5V5
MaxQBiQ9D5V5
PaxDbiQ9D5V5
PRIDEiQ9D5V5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi75717
KEGGimmu:75717
UCSCiuc009pmj.1 mouse

Organism-specific databases

CTDi8065
MGIiMGI:1922967 Cul5

Phylogenomic databases

eggNOGiKOG2285 Eukaryota
COG5647 LUCA
HOGENOMiHOG000007610
HOVERGENiHBG099672
InParanoidiQ9D5V5
KOiK10612
PhylomeDBiQ9D5V5

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi6.3.2.19 3474

Miscellaneous databases

EvolutionaryTraceiQ9D5V5
PROiPR:Q9D5V5
SOURCEiSearch...

Gene expression databases

CleanExiMM_CUL5

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016157 Cullin_CS
IPR016158 Cullin_homology
IPR036317 Cullin_homology_sf
IPR001373 Cullin_N
IPR019559 Cullin_neddylation_domain
IPR016159 Cullin_repeat-like_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00888 Cullin, 1 hit
PF10557 Cullin_Nedd8, 1 hit
SMARTiView protein in SMART
SM00182 CULLIN, 1 hit
SM00884 Cullin_Nedd8, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF74788 SSF74788, 1 hit
SSF75632 SSF75632, 1 hit
PROSITEiView protein in PROSITE
PS01256 CULLIN_1, 1 hit
PS50069 CULLIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCUL5_MOUSE
AccessioniPrimary (citable) accession number: Q9D5V5
Secondary accession number(s): Q8BMQ6, Q8BV53, Q8C098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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