UniProtKB - Q9D5S7 (LRGUK_MOUSE)
Protein
Leucine-rich repeat and guanylate kinase domain-containing protein
Gene
Lrguk
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in multiple aspects of sperm assembly including acrosome attachment, shaping of the sperm head and in the early aspects of axoneme development (PubMed:25781171). Not essential for primary cilium biogenesis (PubMed:28003339).2 Publications
Miscellaneous
Mutagenesis with N-ethyl-N-nitrosourea (ENU) lead to the discovery of the Kaos phenotype. The Kaos mutation results in the conversion of Arg-528 to a stop codon. Homozygous males are oligoasthenoteratospermic and sterile.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 421 – 428 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- guanylate kinase activity Source: GO_Central
GO - Biological processi
- axoneme assembly Source: UniProtKB
- cell differentiation Source: UniProtKB-KW
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Differentiation, Spermatogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Leucine-rich repeat and guanylate kinase domain-containing protein |
Gene namesi | Name:Lrguk |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921604, Lrguk |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
- cilium basal body 1 Publication
Other locations
- acrosome 2 Publications
Note: Localizes to the acrosome and acroplaxome in round spermatids. Localizes to the manchette during spermiogenesis. Also found in the basal body of elongating spermatids, and in primary cilia of somatic cells.2 Publications
Cytoskeleton
- manchette Source: UniProtKB
Cytosol
- cytosol Source: GO_Central
Other locations
- acrosomal vesicle Source: UniProtKB
- cell projection Source: UniProtKB-KW
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoplasmic vesicle, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326409 | 1 – 820 | Leucine-rich repeat and guanylate kinase domain-containing proteinAdd BLAST | 820 |
Proteomic databases
jPOSTi | Q9D5S7 |
MaxQBi | Q9D5S7 |
PaxDbi | Q9D5S7 |
PeptideAtlasi | Q9D5S7 |
PRIDEi | Q9D5S7 |
PTM databases
iPTMneti | Q9D5S7 |
PhosphoSitePlusi | Q9D5S7 |
Expressioni
Tissue specificityi
Highly expressed in the testis (PubMed:25781171). During spermatid development is initially localised to a supra-nuclear region of round spermatids, and is particularly evident at the leading edge of the developing acrosome and acroplaxome. As maturation proceeded and nuclear elongation initiated, LRGUK moves distally to ultimately reside on the microtubules of the manchette. LRGUK is also evident in the sperm basal body and the sperm tail (PubMed:25781171).1 Publication
Developmental stagei
Detectable at low levels from birth, up-regulated at day 14 coincident with the appearance of pachytene spermatocytes, then maximal from day 18 coincident with the appearance of haploid germ cells (PubMed:25781171).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000056215, Expressed in spermatocyte and 61 other tissues |
ExpressionAtlasi | Q9D5S7, baseline and differential |
Genevisiblei | Q9D5S7, MM |
Interactioni
Subunit structurei
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000065146 |
Miscellaneous databases
RNActi | Q9D5S7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 129 – 149 | LRR 1Add BLAST | 21 | |
Repeati | 150 – 171 | LRR 2Add BLAST | 22 | |
Repeati | 172 – 193 | LRR 3Add BLAST | 22 | |
Repeati | 194 – 215 | LRR 4Add BLAST | 22 | |
Repeati | 216 – 237 | LRR 5Add BLAST | 22 | |
Repeati | 238 – 259 | LRR 6Add BLAST | 22 | |
Repeati | 260 – 280 | LRR 7Add BLAST | 21 | |
Repeati | 281 – 302 | LRR 8Add BLAST | 22 | |
Repeati | 303 – 324 | LRR 9Add BLAST | 22 | |
Domaini | 337 – 375 | LRRCTAdd BLAST | 39 | |
Domaini | 414 – 597 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 184 |
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG0531, Eukaryota KOG0707, Eukaryota |
GeneTreei | ENSGT00940000157992 |
HOGENOMi | CLU_019293_0_0_1 |
InParanoidi | Q9D5S7 |
OrthoDBi | 156510at2759 |
TreeFami | TF329158 |
Family and domain databases
Gene3Di | 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS51450, LRR, 10 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9D5S7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAFERNPSR WKGTRFRRGL GASRIAAQAI LSLTEKQSQG RWPSFPLGLK
60 70 80 90 100
SKGTFRSASS YLLHQLIHRS HEAEAEQEEK QQEDGESEES EESEMQNLED
110 120 130 140 150
KYDGILREET VAEAITGLGW SGRGTEQVYL NLNLSHCELV DISILCGYVH
160 170 180 190 200
LQKLNLSGNR IEDLSCVSCM PYLLELNASQ NKLTTFFNFK PPQNLKKVDF
210 220 230 240 250
SSNLISEMYD LSAYHTLTQL ILDNNEIEEI TGLENCISLT HLSLAGNKIT
260 270 280 290 300
TIKGLGTLPI KVLSLSNNMI ETITGLEELK ALQNLDLSHN QISSLQGLEN
310 320 330 340 350
HDLLEVINLE DNKIKELSEI EYIENLPILR VLNLLRNPIQ TKPEYWFFVI
360 370 380 390 400
YMLLRLTELD QQKIKVEEKV FAVNKYDPPP EVVAVQDHMT HVVNSMSQPQ
410 420 430 440 450
RIWDSTLPSL DAPYPMLILT GPAACGKREL AHRLCRQFST YFRYGACHTT
460 470 480 490 500
RPPYFGEGDR VDYHFISQEV FDEMLNMGKF ILTFNYGNHN YGLNRDTIEG
510 520 530 540 550
IARDGLASCI HMELEGVRSL KYSYFEPRYI LVVPMDKEKY EGYLRRKGLF
560 570 580 590 600
SRAEIEIAVS RVDLYVKVNQ KYPGYFDAVI NADDMDIAYQ KLSELIREYL
610 620 630 640 650
GLTETAAKTL APTADTKTSY LKCEDYSRKS STVEFLDSTD RNYFTKLWAK
660 670 680 690 700
LSSKKSPVER ESLHRQHEAA RQALMGKTPR DHTLLFQRGP VPIPTVSGQQ
710 720 730 740 750
YFATIDELQK TFELSDDLFK TPSGTYPETS KDSNISKRYS TYFHTCPWSK
760 770 780 790 800
ELPFQLPEGG ISSRPGSAGS DEVDGALKAL RVASSMQEKV AQHKRLSAIT
810 820
IMDPGSNTKP TLPPIPHGRR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BQA5 | A0A2I3BQA5_MOUSE | Leucine-rich repeat and guanylate k... | Lrguk | 1,340 | Annotation score: | ||
Q8BR18 | Q8BR18_MOUSE | Leucine-rich repeat and guanylate k... | Lrguk | 202 | Annotation score: | ||
F6Z1G4 | F6Z1G4_MOUSE | Leucine-rich repeat and guanylate k... | Lrguk | 72 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014976 mRNA Translation: BAB29648.1 |
CCDSi | CCDS51745.1 |
RefSeqi | NP_083162.1, NM_028886.1 |
Genome annotation databases
Ensembli | ENSMUST00000070189; ENSMUSP00000065146; ENSMUSG00000056215 |
GeneIDi | 74354 |
KEGGi | mmu:74354 |
UCSCi | uc009bgv.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014976 mRNA Translation: BAB29648.1 |
CCDSi | CCDS51745.1 |
RefSeqi | NP_083162.1, NM_028886.1 |
3D structure databases
SMRi | Q9D5S7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000065146 |
PTM databases
iPTMneti | Q9D5S7 |
PhosphoSitePlusi | Q9D5S7 |
Proteomic databases
jPOSTi | Q9D5S7 |
MaxQBi | Q9D5S7 |
PaxDbi | Q9D5S7 |
PeptideAtlasi | Q9D5S7 |
PRIDEi | Q9D5S7 |
Protocols and materials databases
Antibodypediai | 18051, 64 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000070189; ENSMUSP00000065146; ENSMUSG00000056215 |
GeneIDi | 74354 |
KEGGi | mmu:74354 |
UCSCi | uc009bgv.1, mouse |
Organism-specific databases
CTDi | 136332 |
MGIi | MGI:1921604, Lrguk |
Phylogenomic databases
eggNOGi | KOG0531, Eukaryota KOG0707, Eukaryota |
GeneTreei | ENSGT00940000157992 |
HOGENOMi | CLU_019293_0_0_1 |
InParanoidi | Q9D5S7 |
OrthoDBi | 156510at2759 |
TreeFami | TF329158 |
Miscellaneous databases
BioGRID-ORCSi | 74354, 4 hits in 17 CRISPR screens |
ChiTaRSi | Lrguk, mouse |
PROi | PR:Q9D5S7 |
RNActi | Q9D5S7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000056215, Expressed in spermatocyte and 61 other tissues |
ExpressionAtlasi | Q9D5S7, baseline and differential |
Genevisiblei | Q9D5S7, MM |
Family and domain databases
Gene3Di | 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR001611, Leu-rich_rpt IPR032675, LRR_dom_sf IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50052, GUANYLATE_KINASE_2, 1 hit PS51450, LRR, 10 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LRGUK_MOUSE | |
Accessioni | Q9D5S7Primary (citable) accession number: Q9D5S7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot