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Protein

SPRY domain-containing SOCS box protein 1

Gene

Spsb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
SPRY domain-containing SOCS box protein 1
Short name:
SSB-1
Gene namesi
Name:Spsb1
Synonyms:Ssb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1921896 Spsb1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384731 – 273SPRY domain-containing SOCS box protein 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9D5L7
PRIDEiQ9D5L7

PTM databases

iPTMnetiQ9D5L7
PhosphoSitePlusiQ9D5L7

Expressioni

Gene expression databases

BgeeiENSMUSG00000039911 Expressed in 239 organ(s), highest expression level in female gonad
ExpressionAtlasiQ9D5L7 baseline and differential
GenevisibleiQ9D5L7 MM

Interactioni

Subunit structurei

Component of the probable ECS(SPSB1) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SPSB1 (By similarity). Interacts with CUL5, RNF7, ELOB and ELOC (By similarity). Directly interacts with MET tyrosine kinase domain in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction (PubMed:16369487). When phosphorylated, interacts with RASA1 without affecting its stability (By similarity). Interacts (via B30.2/SPRY domain) with PAWR; this interaction is direct and occurs in association with the Elongin BC complex (PubMed:16369487, PubMed:16498413, PubMed:20561531).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAWRQ96IZ02EBI-8821912,EBI-595869From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-29000N
IntActiQ9D5L7, 1 interactor
STRINGi10090.ENSMUSP00000048969

Structurei

3D structure databases

ProteinModelPortaliQ9D5L7
SMRiQ9D5L7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 231B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199
Domaini232 – 273SOCS boxPROSITE-ProRule annotationAdd BLAST42

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The B30.2/SPRY domain is involved in MET and PAWR binding (PubMed:16369487, PubMed:20561531).By similarity2 Publications

Sequence similaritiesi

Belongs to the SPSB family.Curated

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiQ9D5L7
KOiK10343
OMAiMGQKVPG
OrthoDBiEOG091G0DF0
PhylomeDBiQ9D5L7
TreeFamiTF312822

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR003877 SPRY_dom
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9D5L7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGQKVTGGIK TVDMRDPTYR PLKQELQGLD YCKPTRLDLL LDMPPVSYDV
60 70 80 90 100
QLLHSWNNND RSLNVFVKED DKLIFHRHPV AQSTDAIRGK VGYTRGLHVW
110 120 130 140 150
QITWAMRQRG THAVVGVATA DAPLHSVGYT TLVGNNHESW GWDLGRNRLY
160 170 180 190 200
HDGKNQPSKT YPAFLEPDET FIVPDSFLVA LDMDDGTLSF IVDGQYMGVA
210 220 230 240 250
FRGLKGKKLY PVVSAVWGHC EIRMRYLNGL DPEPLPLMDL CRRSVRLALG
260 270
KERLGAIPAL PLPASLKAYL LYQ
Length:273
Mass (Da):30,785
Last modified:June 1, 2001 - v1
Checksum:i2CBA3B50A3240177
GO
Isoform 2 (identifier: Q9D5L7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.

Show »
Length:168
Mass (Da):18,656
Checksum:i4E94AAA0ED006A4A
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A7B3A2A7B3_MOUSE
SPRY domain-containing SOCS box pro...
Spsb1
201Annotation score:
A2A7B2A2A7B2_MOUSE
SPRY domain-containing SOCS box pro...
Spsb1
38Annotation score:
A2A7B0A2A7B0_MOUSE
SPRY domain-containing SOCS box pro...
Spsb1
20Annotation score:
E9Q768E9Q768_MOUSE
SPRY domain-containing SOCS box pro...
Spsb1
39Annotation score:
A2A7B1A2A7B1_MOUSE
SPRY domain-containing SOCS box pro...
Spsb1
36Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11T → S in BAC40957 (Ref. 1) Curated1
Sequence conflicti125H → R in BAC40957 (Ref. 1) Curated1
Sequence conflicti146R → H in BAC40957 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186121 – 105Missing in isoform 2. 1 PublicationAdd BLAST105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403036 mRNA Translation: AAL57355.1
AK015181 mRNA Translation: BAB29738.1
AK003707 mRNA Translation: BAC25049.1
AK030417 mRNA Translation: BAC26954.1
AK040909 mRNA Translation: BAC30739.1
AK089767 mRNA Translation: BAC40957.1
AK160081 mRNA Translation: BAE35613.1
AK172334 mRNA Translation: BAE42952.1
BC046787 mRNA Translation: AAH46787.1
BC057563 mRNA Translation: AAH57563.1
BC089357 mRNA Translation: AAH89357.1
CCDSiCCDS18966.1 [Q9D5L7-1]
RefSeqiNP_083311.1, NM_029035.2 [Q9D5L7-1]
XP_006539297.1, XM_006539234.2 [Q9D5L7-1]
XP_011248646.1, XM_011250344.2 [Q9D5L7-1]
XP_011248647.1, XM_011250345.2 [Q9D5L7-1]
UniGeneiMm.30

Genome annotation databases

EnsembliENSMUST00000038562; ENSMUSP00000048969; ENSMUSG00000039911 [Q9D5L7-1]
ENSMUST00000105684; ENSMUSP00000101309; ENSMUSG00000039911 [Q9D5L7-1]
ENSMUST00000105685; ENSMUSP00000101310; ENSMUSG00000039911 [Q9D5L7-1]
GeneIDi74646
KEGGimmu:74646
UCSCiuc008vxf.1 mouse [Q9D5L7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF403036 mRNA Translation: AAL57355.1
AK015181 mRNA Translation: BAB29738.1
AK003707 mRNA Translation: BAC25049.1
AK030417 mRNA Translation: BAC26954.1
AK040909 mRNA Translation: BAC30739.1
AK089767 mRNA Translation: BAC40957.1
AK160081 mRNA Translation: BAE35613.1
AK172334 mRNA Translation: BAE42952.1
BC046787 mRNA Translation: AAH46787.1
BC057563 mRNA Translation: AAH57563.1
BC089357 mRNA Translation: AAH89357.1
CCDSiCCDS18966.1 [Q9D5L7-1]
RefSeqiNP_083311.1, NM_029035.2 [Q9D5L7-1]
XP_006539297.1, XM_006539234.2 [Q9D5L7-1]
XP_011248646.1, XM_011250344.2 [Q9D5L7-1]
XP_011248647.1, XM_011250345.2 [Q9D5L7-1]
UniGeneiMm.30

3D structure databases

ProteinModelPortaliQ9D5L7
SMRiQ9D5L7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29000N
IntActiQ9D5L7, 1 interactor
STRINGi10090.ENSMUSP00000048969

PTM databases

iPTMnetiQ9D5L7
PhosphoSitePlusiQ9D5L7

Proteomic databases

PaxDbiQ9D5L7
PRIDEiQ9D5L7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038562; ENSMUSP00000048969; ENSMUSG00000039911 [Q9D5L7-1]
ENSMUST00000105684; ENSMUSP00000101309; ENSMUSG00000039911 [Q9D5L7-1]
ENSMUST00000105685; ENSMUSP00000101310; ENSMUSG00000039911 [Q9D5L7-1]
GeneIDi74646
KEGGimmu:74646
UCSCiuc008vxf.1 mouse [Q9D5L7-1]

Organism-specific databases

CTDi80176
MGIiMGI:1921896 Spsb1

Phylogenomic databases

eggNOGiKOG3953 Eukaryota
ENOG410XQC1 LUCA
GeneTreeiENSGT00390000009402
HOGENOMiHOG000230524
HOVERGENiHBG055793
InParanoidiQ9D5L7
KOiK10343
OMAiMGQKVPG
OrthoDBiEOG091G0DF0
PhylomeDBiQ9D5L7
TreeFamiTF312822

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiSpsb1 mouse
PROiPR:Q9D5L7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039911 Expressed in 239 organ(s), highest expression level in female gonad
ExpressionAtlasiQ9D5L7 baseline and differential
GenevisibleiQ9D5L7 MM

Family and domain databases

InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR001496 SOCS_box
IPR003877 SPRY_dom
PfamiView protein in Pfam
PF07525 SOCS_box, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00969 SOCS_box, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50225 SOCS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPSB1_MOUSE
AccessioniPrimary (citable) accession number: Q9D5L7
Secondary accession number(s): Q5FWI0, Q8BJA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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