UniProtKB - Q9D5A0 (SPESP_MOUSE)
Protein
Sperm equatorial segment protein 1
Gene
Spesp1
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in fertilization ability of sperm.2 Publications
GO - Biological processi
- acrosome reaction Source: MGI
- fertilization Source: UniProtKB
- fusion of sperm to egg plasma membrane involved in single fertilization Source: MGI
- multicellular organism development Source: UniProtKB-KW
- sperm-egg recognition Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Names & Taxonomyi
Protein namesi | Recommended name: Sperm equatorial segment protein 1By similarity |
Gene namesi | Name:Spesp1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913962, Spesp1 |
Subcellular locationi
Other locations
- acrosome 1 Publication
Note: Small proacrosomal granules (during the Golgi phase), enlarged acrosomal vesicles (during the cap phase), acrosome (during the elongating phase), equatorial segment of the acrosome (during the maturation phase) (By similarity). After acrosome reaction localizes to the equatorial segment region in both noncapacitated and capacitated, acrosome-reacted sperm (PubMed:25761597).By similarity1 Publication
Other locations
- acrosomal vesicle Source: MGI
Keywords - Cellular componenti
Cytoplasmic vesiclePathology & Biotechi
Disruption phenotypei
Knockout female mice are fertile, while the average number of pups that are fathered by knockout males is significantly lower than that of wild-type fathers.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000042709 | 19 – 399 | Sperm equatorial segment protein 1Add BLAST | 381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 128 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated. In testis there are two predominant forms of 77- and 67-kDa and a form of 47-kDa, whereas in epididymal sperm from caput, corpus, and cauda there are two forms of 47- and 43-kDa. Testis forms contain complex carbohydrate residues. Epididymal sperm forms are N-glycosylated. Then undergoes significant glycosylation in the testis and that the majority of these glycoconjugates are removed by the time sperm reach the caput epididymis.1 Publication
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9D5A0 |
PeptideAtlasi | Q9D5A0 |
PRIDEi | Q9D5A0 |
PTM databases
GlyGeni | Q9D5A0, 1 site |
PhosphoSitePlusi | Q9D5A0 |
Expressioni
Tissue specificityi
Testis specific.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000046846, Expressed in testis and 20 other tissues |
Genevisiblei | Q9D5A0, MM |
Interactioni
Protein-protein interaction databases
IntActi | Q9D5A0, 1 interactor |
STRINGi | 10090.ENSMUSP00000058522 |
Miscellaneous databases
RNActi | Q9D5A0, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 180 | Glu-richAdd BLAST | 45 | |
Compositional biasi | 183 – 226 | Thr-richAdd BLAST | 44 | |
Compositional biasi | 299 – 304 | Poly-Ala | 6 |
Sequence similaritiesi
Belongs to the SPESP1 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SG7W, Eukaryota |
GeneTreei | ENSGT00390000005362 |
HOGENOMi | CLU_787463_0_0_1 |
InParanoidi | Q9D5A0 |
OMAi | TAFWSIK |
OrthoDBi | 1348911at2759 |
PhylomeDBi | Q9D5A0 |
TreeFami | TF337441 |
Family and domain databases
InterProi | View protein in InterPro IPR026743, Equatorial_segment |
PANTHERi | PTHR31667, PTHR31667, 1 hit |
Pfami | View protein in Pfam PF15754, SPESP1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9D5A0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLVVLVALW LWPSSLLAYP TITVLPDEEQ NLNHYVHILQ NLIMSVPTKE
60 70 80 90 100
QDLGKKLSSS RTVDSAEPRS LASKVLLTPG LVSAQDVTPE SDVLIRPVDE
110 120 130 140 150
TTNSRTRGFT LRRRRTQSTA FWSIRPNNIS VVLRTEEPFI EKEPEPELES
160 170 180 190 200
SRLPTEPEPE LEPEPEPVAE SRQMSEPEEE LVTSTTPNKE LTGTSRISSM
210 220 230 240 250
ATQPANTQAT RITVTVKTTS TMDVSTDSED VPQLSGQSEI PSAEDLPGRH
260 270 280 290 300
SLNTRHEDIL KKISNINAEI QQGLLGGNNS PEFKEFIKAS REHLKRSLAL
310 320 330 340 350
AAAAEHKLEQ MYGSNVFPEG RTSDPDNDME MIINMLYNSR SKLSDYFNIK
360 370 380 390
RVPSELREKA SVVNAELRKI LCVDQVEMQS LIKKLLSNNM KILNILNVP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 170 – 171 | ES → DP in BAB29908 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 196 | R → Q in BAB29908 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014843 mRNA Translation: BAB29577.1 AK015620 mRNA Translation: BAB29908.1 BC050754 mRNA Translation: AAH50754.1 |
CCDSi | CCDS23263.1 |
RefSeqi | NP_079997.1, NM_025721.2 |
Genome annotation databases
Ensembli | ENSMUST00000056949; ENSMUSP00000058522; ENSMUSG00000046846 |
GeneIDi | 66712 |
KEGGi | mmu:66712 |
UCSCi | uc009qag.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK014843 mRNA Translation: BAB29577.1 AK015620 mRNA Translation: BAB29908.1 BC050754 mRNA Translation: AAH50754.1 |
CCDSi | CCDS23263.1 |
RefSeqi | NP_079997.1, NM_025721.2 |
3D structure databases
SMRi | Q9D5A0 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9D5A0, 1 interactor |
STRINGi | 10090.ENSMUSP00000058522 |
PTM databases
GlyGeni | Q9D5A0, 1 site |
PhosphoSitePlusi | Q9D5A0 |
Proteomic databases
PaxDbi | Q9D5A0 |
PeptideAtlasi | Q9D5A0 |
PRIDEi | Q9D5A0 |
Protocols and materials databases
Antibodypediai | 51298, 78 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000056949; ENSMUSP00000058522; ENSMUSG00000046846 |
GeneIDi | 66712 |
KEGGi | mmu:66712 |
UCSCi | uc009qag.1, mouse |
Organism-specific databases
CTDi | 246777 |
MGIi | MGI:1913962, Spesp1 |
Phylogenomic databases
eggNOGi | ENOG502SG7W, Eukaryota |
GeneTreei | ENSGT00390000005362 |
HOGENOMi | CLU_787463_0_0_1 |
InParanoidi | Q9D5A0 |
OMAi | TAFWSIK |
OrthoDBi | 1348911at2759 |
PhylomeDBi | Q9D5A0 |
TreeFami | TF337441 |
Miscellaneous databases
BioGRID-ORCSi | 66712, 1 hit in 18 CRISPR screens |
PROi | PR:Q9D5A0 |
RNActi | Q9D5A0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046846, Expressed in testis and 20 other tissues |
Genevisiblei | Q9D5A0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026743, Equatorial_segment |
PANTHERi | PTHR31667, PTHR31667, 1 hit |
Pfami | View protein in Pfam PF15754, SPESP1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPESP_MOUSE | |
Accessioni | Q9D5A0Primary (citable) accession number: Q9D5A0 Secondary accession number(s): Q9D5X8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families