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Protein

Splicing factor 3A subunit 3

Gene

Sf3a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 3
Alternative name(s):
SF3a60
Spliceosome-associated protein 61
Short name:
SAP 61
Gene namesi
Name:Sf3a3
Synonyms:Sap61
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922312 Sf3a3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743191 – 501Splicing factor 3A subunit 3Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei54PhosphoserineBy similarity1
Modified residuei121PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei299PhosphoserineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei367PhosphoserineBy similarity1
Modified residuei369PhosphoserineBy similarity1
Modified residuei475PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9D554
MaxQBiQ9D554
PaxDbiQ9D554
PRIDEiQ9D554

PTM databases

iPTMnetiQ9D554
PhosphoSitePlusiQ9D554
SwissPalmiQ9D554

Expressioni

Gene expression databases

BgeeiENSMUSG00000028902 Expressed in 296 organ(s), highest expression level in cochlea
CleanExiMM_SF3A3
ExpressionAtlasiQ9D554 baseline and differential
GenevisibleiQ9D554 MM

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A1, through its N-terminal region (By similarity).By similarity

Protein-protein interaction databases

BioGridi217189, 4 interactors
IntActiQ9D554, 6 interactors
MINTiQ9D554
STRINGi10090.ENSMUSP00000030734

Structurei

3D structure databases

ProteinModelPortaliQ9D554
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 362Poly-Glu9

Sequence similaritiesi

Belongs to the SF3A3 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri406 – 437Matrin-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2636 Eukaryota
COG5188 LUCA
GeneTreeiENSGT00530000063402
HOGENOMiHOG000160935
HOVERGENiHBG054452
InParanoidiQ9D554
KOiK12827
OMAiEICGNYV
OrthoDBiEOG091G059G
PhylomeDBiQ9D554
TreeFamiTF315227

Family and domain databases

InterProiView protein in InterPro
IPR024598 DUF3449
IPR000690 Matrin/U1-C_Znf_C2H2
IPR031776 SF3A3
IPR031774 SF3A3_dom
IPR021966 SF3a60_bindingd
PANTHERiPTHR12786:SF2 PTHR12786:SF2, 1 hit
PfamiView protein in Pfam
PF11931 DUF3449, 1 hit
PF16837 SF3A3, 1 hit
PF12108 SF3a60_bindingd, 1 hit
PROSITEiView protein in PROSITE
PS50171 ZF_MATRIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D554-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY
60 70 80 90 100
MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN
110 120 130 140 150
EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI
160 170 180 190 200
NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV
210 220 230 240 250
KPLQDQNELF GKIQTDFEKK WDNGTFPGWP KETSSALTHA GAHLDLSAFS
260 270 280 290 300
SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL
310 320 330 340 350
FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRQL THENVQRKQA
360 370 380 390 400
RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH
410 420 430 440 450
GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT
460 470 480 490 500
QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL

L
Length:501
Mass (Da):58,842
Last modified:August 29, 2001 - v2
Checksum:i46EE0928FC8271D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC009141 mRNA Translation: AAH09141.1
AK015776 mRNA Translation: BAB29971.1
CCDSiCCDS38875.1
RefSeqiNP_083433.1, NM_029157.3
UniGeneiMm.25779

Genome annotation databases

EnsembliENSMUST00000030734; ENSMUSP00000030734; ENSMUSG00000028902
GeneIDi75062
KEGGimmu:75062
UCSCiuc008uqx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC009141 mRNA Translation: AAH09141.1
AK015776 mRNA Translation: BAB29971.1
CCDSiCCDS38875.1
RefSeqiNP_083433.1, NM_029157.3
UniGeneiMm.25779

3D structure databases

ProteinModelPortaliQ9D554
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217189, 4 interactors
IntActiQ9D554, 6 interactors
MINTiQ9D554
STRINGi10090.ENSMUSP00000030734

PTM databases

iPTMnetiQ9D554
PhosphoSitePlusiQ9D554
SwissPalmiQ9D554

Proteomic databases

EPDiQ9D554
MaxQBiQ9D554
PaxDbiQ9D554
PRIDEiQ9D554

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030734; ENSMUSP00000030734; ENSMUSG00000028902
GeneIDi75062
KEGGimmu:75062
UCSCiuc008uqx.1 mouse

Organism-specific databases

CTDi10946
MGIiMGI:1922312 Sf3a3

Phylogenomic databases

eggNOGiKOG2636 Eukaryota
COG5188 LUCA
GeneTreeiENSGT00530000063402
HOGENOMiHOG000160935
HOVERGENiHBG054452
InParanoidiQ9D554
KOiK12827
OMAiEICGNYV
OrthoDBiEOG091G059G
PhylomeDBiQ9D554
TreeFamiTF315227

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

PROiPR:Q9D554
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028902 Expressed in 296 organ(s), highest expression level in cochlea
CleanExiMM_SF3A3
ExpressionAtlasiQ9D554 baseline and differential
GenevisibleiQ9D554 MM

Family and domain databases

InterProiView protein in InterPro
IPR024598 DUF3449
IPR000690 Matrin/U1-C_Znf_C2H2
IPR031776 SF3A3
IPR031774 SF3A3_dom
IPR021966 SF3a60_bindingd
PANTHERiPTHR12786:SF2 PTHR12786:SF2, 1 hit
PfamiView protein in Pfam
PF11931 DUF3449, 1 hit
PF16837 SF3A3, 1 hit
PF12108 SF3a60_bindingd, 1 hit
PROSITEiView protein in PROSITE
PS50171 ZF_MATRIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A3_MOUSE
AccessioniPrimary (citable) accession number: Q9D554
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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