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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase gamma

Gene

Agpat3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:15367102). Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (By similarity). Has a preference for arachidonoyl-CoA as a donor (PubMed:19114731). Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (PubMed:19114731).By similarity2 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

In males, activity increases in an age-dependent fashion, maybe derived from the induction by sex-hormones.1 Publication

Kineticsi

  1. KM=15.9 µM for arachidonoyl-CoA1 Publication
  2. KM=26.3 µM for palmitoyl-LPA1 Publication
  1. Vmax=50.4 nmol/min/mg enzyme toward arachidonoyl-CoA1 Publication
  2. Vmax=21.8 nmol/min/mg enzyme toward palmitoyl-LPA1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.51 3474
ReactomeiR-MMU-1483166 Synthesis of PA
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
UniPathwayiUPA00557; UER00613

Chemistry databases

SwissLipidsiSLP:000000097

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 3
Short name:
1-AGP acyltransferase 3
Short name:
1-AGPAT 3
Lysophosphatidic acid acyltransferase gamma
Short name:
LPAAT-gamma
Gene namesi
Name:Agpat3Imported
Synonyms:Lpaat31 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1336186 Agpat3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 316LumenalSequence analysisAdd BLAST171
Transmembranei317 – 339HelicalSequence analysisAdd BLAST23
Topological domaini340 – 376CytoplasmicSequence analysisAdd BLAST37

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi96H → A: Loss of LPA acyltransferase and LPI acyltransferase activities. 1 Publication1
Mutagenesisi101D → A: Loss of LPA acyltransferase and LPI acyltransferase activities. 1 Publication1
Mutagenesisi176E → A: Loss of LPA acyltransferase and LPI acyltransferase activities. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002081951 – 3761-acyl-sn-glycerol-3-phosphate acyltransferase gammaAdd BLAST376

Proteomic databases

EPDiQ9D517
MaxQBiQ9D517
PaxDbiQ9D517
PeptideAtlasiQ9D517
PRIDEiQ9D517

PTM databases

iPTMnetiQ9D517
PhosphoSitePlusiQ9D517
SwissPalmiQ9D517

Expressioni

Tissue specificityi

Widely expressed. Mainly expressed in testis, kidney and liver (at protein level).1 Publication

Inductioni

Up-regulated in the heart by clofibrate, a PPAR-alpha agonist.1 Publication

Gene expression databases

BgeeiENSMUSG00000001211
CleanExiMM_AGPAT3
ExpressionAtlasiQ9D517 baseline and differential
GenevisibleiQ9D517 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001240

Structurei

3D structure databases

ProteinModelPortaliQ9D517
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 101HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOGENOMiHOG000006110
HOVERGENiHBG008205
InParanoidiQ9D517
KOiK13523
OMAiFLFWATV
OrthoDBiEOG091G0C4Z
PhylomeDBiQ9D517
TreeFamiTF314065

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLAYLKTQ FVVHLLIGFV FVVSGLIINF TQLCTLALWP ISKHLYRRIN
60 70 80 90 100
CRLAYSLWSQ LVMLLEWWSC TECTLFTDQA TVDHFGKEHV VVILNHNFEI
110 120 130 140 150
DFLCGWTMCE RFGVLGSSKV LAKRELLCVP LIGWTWYFLE IVFCKRKWEE
160 170 180 190 200
DRDTVIEGLR RLADYPEYMW FLLYCEGTRF TETKHRISME VAASKGLPPL
210 220 230 240 250
KYHLLPRTKG FTTAVQCLRG TVAAIYDVTL NFRGNKNPSL LGILYGKKYE
260 270 280 290 300
ADMCVRRFPL EDIPADETSA AQWLHKLYQE KDALQEMYKQ KGVFPGEQFK
310 320 330 340 350
PARRPWTLLN FLCWATILLS PLFSFVLGVF ASGSPLLILT FLGFVGAASF
360 370
GVRRLIGVTE IEKGSSYGNQ ELKKKE
Length:376
Mass (Da):43,296
Last modified:March 1, 2003 - v2
Checksum:i6F8C8970404B2EC1
GO

Sequence cautioni

The sequence BAC27043 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21F → V in AAH52382 (PubMed:15489334).Curated1
Sequence conflicti21F → V in AAH58519 (PubMed:15489334).Curated1
Sequence conflicti249Y → N in BAC27043 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY167588 mRNA Translation: AAN75574.1
AB377215 mRNA Translation: BAH59614.1
AK015906 mRNA Translation: BAB30025.2
AK030607 mRNA Translation: BAC27043.1 Different initiation.
AK075715 mRNA Translation: BAC35905.1
AK076414 mRNA Translation: BAC36329.1
CH466553 Genomic DNA Translation: EDL31738.1
CH466553 Genomic DNA Translation: EDL31739.1
CH466553 Genomic DNA Translation: EDL31740.1
CH466553 Genomic DNA Translation: EDL31742.1
BC052382 mRNA Translation: AAH52382.1
BC058519 mRNA Translation: AAH58519.1
CCDSiCCDS23963.1
RefSeqiNP_443747.2, NM_053014.3
XP_006513779.1, XM_006513716.2
XP_006513780.1, XM_006513717.3
XP_006513781.1, XM_006513718.1
XP_006513782.1, XM_006513719.2
XP_006513783.1, XM_006513720.1
XP_006513784.1, XM_006513721.1
XP_006513785.1, XM_006513722.3
XP_006513786.1, XM_006513723.3
UniGeneiMm.141230

Genome annotation databases

EnsembliENSMUST00000001240; ENSMUSP00000001240; ENSMUSG00000001211
ENSMUST00000105387; ENSMUSP00000101026; ENSMUSG00000001211
ENSMUST00000105388; ENSMUSP00000101027; ENSMUSG00000001211
ENSMUST00000105389; ENSMUSP00000101028; ENSMUSG00000001211
ENSMUST00000105390; ENSMUSP00000101029; ENSMUSG00000001211
ENSMUST00000166360; ENSMUSP00000132954; ENSMUSG00000001211
GeneIDi28169
KEGGimmu:28169
UCSCiuc007fxo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPLCC_MOUSE
AccessioniPrimary (citable) accession number: Q9D517
Secondary accession number(s): C4B4E7, Q7TT39, Q8BST2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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