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Protein

Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2

Gene

Sohlh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation (PubMed:22056784). Coordinates oocyte differentiation without affecting meiosis I (PubMed:28504655).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Gene namesi
Name:Sohlh2
Synonyms:Tohlh2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1921684 Sohlh2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Knockout mice are infertile. Infertility could not be rescued by SOHLH1 or SOHLH2 transgene expression due to a lack of expression of either genes in rescued animals.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003157011 – 467Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2Add BLAST467

Proteomic databases

MaxQBiQ9D489
PaxDbiQ9D489
PRIDEiQ9D489

PTM databases

PhosphoSitePlusiQ9D489

Expressioni

Tissue specificityi

Preferentially expressed in the adult ovary and testis. Expressed in the majority of spermatogonia in adult animals, but not in the most undifferentiated spermatogonial population (PubMed:22056784).1 Publication

Developmental stagei

In the embryonic gonads it is expressed as early as E13.5 and later. In 3-day-old postnatal ovaries, it is expressed within oocytes in germ cell clusters and primordial follicles. In the adult, it is expressed only in small follicles, primary primordial and primary follicles. In testis, it is exclusively present in spermatogonia. Present at postnatal day 5, when spermatogonia stem cells differentiate into type A spermatogonia. Absent in Sertoli cells or spermatocytes (at protein level). Expressed in the germline as early as E12.5 and precedes SOHLH1 protein expression, which occurred circa E15.5. SOHLH1 appearance at E15.5 correlates with SOHLH2 translocation from the cytoplasm into the nucleus (PubMed:28504655).2 Publications

Inductioni

Up-regulated in mice lacking Sohlh1.

Gene expression databases

BgeeiENSMUSG00000027794 Expressed in 24 organ(s), highest expression level in embryo
GenevisibleiQ9D489 MM

Interactioni

Subunit structurei

Forms both hetero- and homodimers with SOHLH1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029369

Structurei

3D structure databases

ProteinModelPortaliQ9D489
SMRiQ9D489
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini200 – 251bHLHPROSITE-ProRule annotationAdd BLAST52

Phylogenomic databases

eggNOGiENOG410IUEE Eukaryota
ENOG410ZGUF LUCA
GeneTreeiENSGT00390000016050
HOGENOMiHOG000070147
HOVERGENiHBG103652
InParanoidiQ9D489
OMAiLPQHCNS
OrthoDBiEOG091G08ZT
PhylomeDBiQ9D489
TreeFamiTF336841

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR032669 SOHLH2
PANTHERiPTHR16223:SF16 PTHR16223:SF16, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9D489-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADRISTGEL GRRPGQGRVN LLLVGDATRY FLAGSMQKFF PSTAQITLTI
60 70 80 90 100
SNVKKVAVLL AANSFDIIFL KVTSTLTAEE QEAAKLIRSG KKKNTHLLFA
110 120 130 140 150
FVIPERLKGY VSEYGADISF SEPLTLEKVN TVIHYWKTYF TNTDMENTEL
160 170 180 190 200
PPECRLYFQT SRSELGGRFS TDMFLCSELL KNDARLGLKA PLSSLDKSKQ
210 220 230 240 250
ASFLHSTKEK LRRERIKSCC EQLRTLLPYV KGRKSDVASV IEATVDYVKQ
260 270 280 290 300
VRESLSPAIM AQVTEAIQNN RRFSKRQMPI ELFLPFSATS QREDAMLTSA
310 320 330 340 350
FSPVQEIQLL ADRGLNVYSM TAAGGALEGA VRGQPGSVSE SSIEDLYKTR
360 370 380 390 400
VPSTARSLNS FHSVKYTSGT VSPHEAAART NQNISTYLPP TAPSVSNFIP
410 420 430 440 450
QHCNAMLCPA RPTSPNCLCT PGHELAASSR AASASIFRGF RAASASDHQA
460
SQPPALPSPQ PHDSSYF
Length:467
Mass (Da):51,325
Last modified:January 15, 2008 - v2
Checksum:iAC29D2ACA2EDD0AE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13 – 14RP → SV in BAB30389 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ086115 mRNA Translation: AAY86077.1
AK016704 mRNA Translation: BAB30389.1
BC120917 mRNA Translation: AAI20918.1
BC120918 mRNA Translation: AAI20919.1
CCDSiCCDS17358.1
RefSeqiNP_083213.2, NM_028937.3
UniGeneiMm.25514

Genome annotation databases

EnsembliENSMUST00000029369; ENSMUSP00000029369; ENSMUSG00000027794
GeneIDi74434
KEGGimmu:74434
UCSCiuc008pgi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ086115 mRNA Translation: AAY86077.1
AK016704 mRNA Translation: BAB30389.1
BC120917 mRNA Translation: AAI20918.1
BC120918 mRNA Translation: AAI20919.1
CCDSiCCDS17358.1
RefSeqiNP_083213.2, NM_028937.3
UniGeneiMm.25514

3D structure databases

ProteinModelPortaliQ9D489
SMRiQ9D489
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029369

PTM databases

PhosphoSitePlusiQ9D489

Proteomic databases

MaxQBiQ9D489
PaxDbiQ9D489
PRIDEiQ9D489

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029369; ENSMUSP00000029369; ENSMUSG00000027794
GeneIDi74434
KEGGimmu:74434
UCSCiuc008pgi.2 mouse

Organism-specific databases

CTDi54937
MGIiMGI:1921684 Sohlh2

Phylogenomic databases

eggNOGiENOG410IUEE Eukaryota
ENOG410ZGUF LUCA
GeneTreeiENSGT00390000016050
HOGENOMiHOG000070147
HOVERGENiHBG103652
InParanoidiQ9D489
OMAiLPQHCNS
OrthoDBiEOG091G08ZT
PhylomeDBiQ9D489
TreeFamiTF336841

Miscellaneous databases

PROiPR:Q9D489
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027794 Expressed in 24 organ(s), highest expression level in embryo
GenevisibleiQ9D489 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR032669 SOHLH2
PANTHERiPTHR16223:SF16 PTHR16223:SF16, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSOLH2_MOUSE
AccessioniPrimary (citable) accession number: Q9D489
Secondary accession number(s): Q4JI40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 7, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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