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Protein

Rho-related GTP-binding protein RhoH

Gene

Rhoh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils (By similarity). Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18GTPBy similarity8
Nucleotide bindingi58 – 62GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.2 3474
ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoH
Gene namesi
Name:Rhoh
Synonyms:Arhh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921984 Rhoh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show impaired passive systemic anaphylaxis and histamine release upon challenge with the specific antigen and impaired T-cell differentiation.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73Y → F: Abolishes interaction with ZAP70; when associated with F-83. 1 Publication1
Mutagenesisi83Y → F: Abolishes interaction with ZAP70; when associated with F-73. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988681 – 188Rho-related GTP-binding protein RhoHAdd BLAST188
PropeptideiPRO_0000281219189 – 191Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Cysteine methyl esterBy similarity1
Lipidationi188S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ9D3G9
MaxQBiQ9D3G9
PaxDbiQ9D3G9
PRIDEiQ9D3G9

PTM databases

iPTMnetiQ9D3G9
PhosphoSitePlusiQ9D3G9

Expressioni

Tissue specificityi

Expression is widespread in hematopoietic cells, including in bone marrow progenitor cells and in differentiated myeloid as well as lymphoid cells. Expressed at high levels in the thymus and mast cells, found in spleen and low-density bone marrow (LDBM) cells and is detected at a low level in neutrophils. In the thymus it is detected in thymocytes of the thymic cortex but not in non-lymphoid cells of fibrovascular and fibroadipose tissues. Expressed in T-cells, B-cells and mast cells.2 Publications

Developmental stagei

Expressed at all stages of thymocyte development, with relative peaks at the DN3 and DP stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000029204 Expressed in 105 organ(s), highest expression level in bone marrow
CleanExiMM_RHOH
ExpressionAtlasiQ9D3G9 baseline and differential
GenevisibleiQ9D3G9 MM

Interactioni

Subunit structurei

Interacts with GDI1 and GDI2 (By similarity). Interacts with ZAP70 (via SH2 domains) and the interaction is enhanced by its phosphorylation by LCK. Interacts with SYK and the interaction is enhanced by its phosphorylation by FYN.By similarity2 Publications

Protein-protein interaction databases

BioGridi216981, 1 interactor
IntActiQ9D3G9, 1 interactor
STRINGi10090.ENSMUSP00000031106

Structurei

3D structure databases

ProteinModelPortaliQ9D3G9
SMRiQ9D3G9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 86Interaction with ZAP701 PublicationAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi33 – 41Effector regionBy similarity9

Domaini

The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00920000149167
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9D3G9
KOiK07873
OMAiCINAIEG
OrthoDBiEOG091G0KVT
PhylomeDBiQ9D3G9
TreeFamiTF331219

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D3G9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSSIKCVLV GDSAVGKTSL LVRFTSETFP EAYKPTVYEN TGVDVFMDGI
60 70 80 90 100
QISLGLWDTA GNDAFRSIRP LSYQQADVVL MCYSVANHNS FLNLKNKWIS
110 120 130 140 150
EIRSNLPCTP VLVVATQTDQ REVGPHRASC INAIEGKRLA QDVRAKGYLE
160 170 180 190
CSALSNRGVQ QVFECAVRTA VNQARRRNRR KLFSINECKI F
Length:191
Mass (Da):21,324
Last modified:June 1, 2001 - v1
Checksum:i18845201F647D539
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017885 mRNA Translation: BAB30987.1
CCDSiCCDS39100.1
RefSeqiNP_001074574.1, NM_001081105.1
XP_011239081.1, XM_011240779.2
UniGeneiMm.20323

Genome annotation databases

EnsembliENSMUST00000031106; ENSMUSP00000031106; ENSMUSG00000029204
ENSMUST00000201533; ENSMUSP00000143810; ENSMUSG00000029204
GeneIDi74734
KEGGimmu:74734
UCSCiuc008xoc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017885 mRNA Translation: BAB30987.1
CCDSiCCDS39100.1
RefSeqiNP_001074574.1, NM_001081105.1
XP_011239081.1, XM_011240779.2
UniGeneiMm.20323

3D structure databases

ProteinModelPortaliQ9D3G9
SMRiQ9D3G9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216981, 1 interactor
IntActiQ9D3G9, 1 interactor
STRINGi10090.ENSMUSP00000031106

PTM databases

iPTMnetiQ9D3G9
PhosphoSitePlusiQ9D3G9

Proteomic databases

EPDiQ9D3G9
MaxQBiQ9D3G9
PaxDbiQ9D3G9
PRIDEiQ9D3G9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031106; ENSMUSP00000031106; ENSMUSG00000029204
ENSMUST00000201533; ENSMUSP00000143810; ENSMUSG00000029204
GeneIDi74734
KEGGimmu:74734
UCSCiuc008xoc.1 mouse

Organism-specific databases

CTDi399
MGIiMGI:1921984 Rhoh

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00920000149167
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9D3G9
KOiK07873
OMAiCINAIEG
OrthoDBiEOG091G0KVT
PhylomeDBiQ9D3G9
TreeFamiTF331219

Enzyme and pathway databases

BRENDAi3.6.5.2 3474
ReactomeiR-MMU-194840 Rho GTPase cycle

Miscellaneous databases

PROiPR:Q9D3G9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029204 Expressed in 105 organ(s), highest expression level in bone marrow
CleanExiMM_RHOH
ExpressionAtlasiQ9D3G9 baseline and differential
GenevisibleiQ9D3G9 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHOH_MOUSE
AccessioniPrimary (citable) accession number: Q9D3G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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