UniProtKB - Q9D3E6 (STAG1_MOUSE)
Protein
Cohesin subunit SA-1
Gene
Stag1
Organism
Mus musculus (Mouse)
Status
Functioni
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis (By similarity).By similarity
GO - Molecular functioni
- chromatin binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- mitotic spindle assembly Source: MGI
- sister chromatid cohesion Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-2467813, Separation of Sister Chromatids R-MMU-2468052, Establishment of Sister Chromatid Cohesion R-MMU-2470946, Cohesin Loading onto Chromatin R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-3108214, SUMOylation of DNA damage response and repair proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Cohesin subunit SA-1Alternative name(s): SCC3 homolog 1 Stromal antigen 1 |
Gene namesi | Name:Stag1 Synonyms:Sa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098658, Stag1 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Other locations
- Chromosome By similarity
Note: Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation (By similarity).By similarity
Cytoskeleton
- mitotic spindle pole Source: MGI
Nucleus
- nuclear body Source: MGI
- nuclear matrix Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- chromatin Source: MGI
- cohesin complex Source: MGI
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120183 | 1 – 1258 | Cohesin subunit SA-1Add BLAST | 1258 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | PhosphoserineBy similarity | 1 | |
Modified residuei | 756 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1062 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1065 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1093 | PhosphoserineBy similarity | 1 | |
Cross-linki | 1161 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Phosphorylated by PLK1. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9D3E6 |
MaxQBi | Q9D3E6 |
PaxDbi | Q9D3E6 |
PeptideAtlasi | Q9D3E6 |
PRIDEi | Q9D3E6 |
PTM databases
iPTMneti | Q9D3E6 |
PhosphoSitePlusi | Q9D3E6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037286, Expressed in forelimb bud and 309 other tissues |
ExpressionAtlasi | Q9D3E6, baseline and differential |
Genevisiblei | Q9D3E6, MM |
Interactioni
Subunit structurei
Cohesin complexes are composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein (STAG1, STAG2 or STAG3). In cohesin complexes, STAG1 is mutually exclusive with STAG2 and STAG3.
Interacts directly with RAD21 in cohesin complex.
Found in a cohesin complex with SMC1A, SMC3 and RAD21.
By similarityProtein-protein interaction databases
BioGRIDi | 203517, 3 interactors |
IntActi | Q9D3E6, 3 interactors |
MINTi | Q9D3E6 |
STRINGi | 10090.ENSMUSP00000116205 |
Miscellaneous databases
RNActi | Q9D3E6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 296 – 381 | SCDPROSITE-ProRule annotationAdd BLAST | 86 |
Sequence similaritiesi
Belongs to the SCC3 family.Curated
Phylogenomic databases
eggNOGi | KOG2011, Eukaryota |
GeneTreei | ENSGT00950000182972 |
InParanoidi | Q9D3E6 |
OrthoDBi | 167826at2759 |
PhylomeDBi | Q9D3E6 |
TreeFami | TF314604 |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR039662, Cohesin_Scc3/SA IPR020839, SCD IPR013721, STAG |
PANTHERi | PTHR11199, PTHR11199, 1 hit |
Pfami | View protein in Pfam PF08514, STAG, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS51425, SCD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
Q9D3E6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITSELPVLQ DSTNETTAHS DAGSELEETE VKGKRKRGRP GRPPSTNKKP
60 70 80 90 100
RKSPGEKSRI EAGIRGAGRG RANGHPQQNG DGDPVTLFEV VKLGKSAMQS
110 120 130 140 150
VVDDWIELYK QDRDIALLDL INFFIQCSGC RGTVRIEMFR NMQNAEIIRK
160 170 180 190 200
MTEEFDEDSG DYPLTMPGPQ WKKFRSNFCE FIGVLIRQCQ YSIIYDEYMM
210 220 230 240 250
DTVISLLTGL SDSQVRAFRH TSTLAAMKLM TALVNVALNL SIHQDNTQRQ
260 270 280 290 300
YEAERNKMIG KRANERLELL LQKRKELQEN QDEIENMMNS IFKGIFVHRY
310 320 330 340 350
RDAIAEIRAI CIEEIGVWMK MYSDAFLNDS YLKYVGWTLH DRQGEVRLKC
360 370 380 390 400
LKALQSLYTN RELFPKLELF TNRFKDRIVS MTLDKEYDVA VEAIRLVTLI
410 420 430 440 450
LHGSEEALSN EDCENVYHLV YSAHRPVAVA AGEFLHKKLF SRHDPQAEEA
460 470 480 490 500
LAKRRGRNSP NGNLIRMLVL FFLESELHEH AAYLVDSLWE SSQELLKDWE
510 520 530 540 550
CMTELLLEEP VQGEEAMSDR QESALIELMV CTIRQAAEAH PPVGRGTGKR
560 570 580 590 600
VLTAKERKTQ IDDRNKLTEH FIITLPMLLS KYSADAEKVA NLLQIPQYFD
610 620 630 640 650
LEIYSTGRME KHLDALLKQI KFVVEKHVES DVLEACSKTY SILCSEEYTI
660 670 680 690 700
QNRVDIARSQ LIDEFVDRFN HSVEDLLQEG EEADDDDIYN VLSTLKRLTS
710 720 730 740 750
FHNAHDLTKW DLFGNCYRLL KTGIEHGAMP EQIVVQALQC SHYSILWQLV
760 770 780 790 800
KITDGSPSKE DLLVLRKTVK SFLAVCQQCL SNVNTPVKEQ AFMLLCDLLM
810 820 830 840 850
IFSHQLMTGG REGLQPLVFN PDTGLQSELL SFVMDHVFID QDEENQSMEG
860 870 880 890 900
DEEDEANKIE ALHKRRNLLA AFSKLIIYDI VDMHAAADIF KHYMKYYNDY
910 920 930 940 950
GDIIKETLSK TRQIDKIQCA KTLILSLQQL FNELVQEQGP NLDRTSAHVS
960 970 980 990 1000
GIKELARRFA LTFGLDQIKT REAVATLHKD GIEFAFKYQN QKGQEYPPPN
1010 1020 1030 1040 1050
LAFLEVLSEF SSKLLRQDKK TVHSYLEKFL TEQMMERRED VWLPLISYRN
1060 1070 1080 1090 1100
SLVTGGEDDR MSVNSGSSSS KTSSVRSKKG RPPLHRKRVE DESLDNTWLN
1110 1120 1130 1140 1150
RTDTMIQTPG PLPTPQLTST VLRENSRPMG EQIQEPESEH GSEPDFLHNP
1160 1170 1180 1190 1200
QMQISWLGQP KLEDLNRKDR TGMNYMKVRA GVRHAVRGLM EEDAEPIFED
1210 1220 1230 1240 1250
VMMSSRSQLE DMNEEFEDTM VIDLPPSRNR RERAELRPDF FDSAAIIEDD
SGFGMPMF
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WHU7 | F8WHU7_MOUSE | Cohesin subunit SA-1 | Stag1 | 1,221 | Annotation score: | ||
D6REF2 | D6REF2_MOUSE | Cohesin subunit SA-1 | Stag1 | 424 | Annotation score: | ||
D6RHQ7 | D6RHQ7_MOUSE | Cohesin subunit SA-1 | Stag1 | 306 | Annotation score: | ||
D3Z1T1 | D3Z1T1_MOUSE | Cohesin subunit SA-1 | Stag1 | 144 | Annotation score: | ||
F6XQW1 | F6XQW1_MOUSE | Cohesin subunit SA-1 | Stag1 | 869 | Annotation score: | ||
F6TNY1 | F6TNY1_MOUSE | Cohesin subunit SA-1 | Stag1 | 285 | Annotation score: | ||
D6RH50 | D6RH50_MOUSE | Cohesin subunit SA-1 | Stag1 | 174 | Annotation score: | ||
Q8C918 | Q8C918_MOUSE | Cohesin subunit SA-1 | Stag1 | 196 | Annotation score: | ||
D3YYD4 | D3YYD4_MOUSE | Cohesin subunit SA-1 | Stag1 | 50 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 – 25 | AGSE → HGRQ in CAA99733 (PubMed:9305759).Curated | 4 | |
Sequence conflicti | 97 | A → R in CAA99733 (PubMed:9305759).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75332 mRNA Translation: CAA99733.1 AK017978 mRNA Translation: BAB31022.1 Sequence problems. BC062954 mRNA Translation: AAH62954.1 |
CCDSi | CCDS23441.1 |
PIRi | T30252 |
RefSeqi | NP_033308.2, NM_009282.3 XP_006510986.1, XM_006510923.2 XP_006510987.1, XM_006510924.2 |
Genome annotation databases
Ensembli | ENSMUST00000129269; ENSMUSP00000116205; ENSMUSG00000037286 |
GeneIDi | 20842 |
KEGGi | mmu:20842 |
UCSCi | uc009rfa.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75332 mRNA Translation: CAA99733.1 AK017978 mRNA Translation: BAB31022.1 Sequence problems. BC062954 mRNA Translation: AAH62954.1 |
CCDSi | CCDS23441.1 |
PIRi | T30252 |
RefSeqi | NP_033308.2, NM_009282.3 XP_006510986.1, XM_006510923.2 XP_006510987.1, XM_006510924.2 |
3D structure databases
SMRi | Q9D3E6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203517, 3 interactors |
IntActi | Q9D3E6, 3 interactors |
MINTi | Q9D3E6 |
STRINGi | 10090.ENSMUSP00000116205 |
PTM databases
iPTMneti | Q9D3E6 |
PhosphoSitePlusi | Q9D3E6 |
Proteomic databases
EPDi | Q9D3E6 |
MaxQBi | Q9D3E6 |
PaxDbi | Q9D3E6 |
PeptideAtlasi | Q9D3E6 |
PRIDEi | Q9D3E6 |
Protocols and materials databases
Antibodypediai | 17829, 253 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000129269; ENSMUSP00000116205; ENSMUSG00000037286 |
GeneIDi | 20842 |
KEGGi | mmu:20842 |
UCSCi | uc009rfa.1, mouse |
Organism-specific databases
CTDi | 10274 |
MGIi | MGI:1098658, Stag1 |
Phylogenomic databases
eggNOGi | KOG2011, Eukaryota |
GeneTreei | ENSGT00950000182972 |
InParanoidi | Q9D3E6 |
OrthoDBi | 167826at2759 |
PhylomeDBi | Q9D3E6 |
TreeFami | TF314604 |
Enzyme and pathway databases
Reactomei | R-MMU-2467813, Separation of Sister Chromatids R-MMU-2468052, Establishment of Sister Chromatid Cohesion R-MMU-2470946, Cohesin Loading onto Chromatin R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-3108214, SUMOylation of DNA damage response and repair proteins |
Miscellaneous databases
BioGRID-ORCSi | 20842, 5 hits in 17 CRISPR screens |
ChiTaRSi | Stag1, mouse |
PROi | PR:Q9D3E6 |
RNActi | Q9D3E6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037286, Expressed in forelimb bud and 309 other tissues |
ExpressionAtlasi | Q9D3E6, baseline and differential |
Genevisiblei | Q9D3E6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR039662, Cohesin_Scc3/SA IPR020839, SCD IPR013721, STAG |
PANTHERi | PTHR11199, PTHR11199, 1 hit |
Pfami | View protein in Pfam PF08514, STAG, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS51425, SCD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STAG1_MOUSE | |
Accessioni | Q9D3E6Primary (citable) accession number: Q9D3E6 Secondary accession number(s): O08982, Q6P5D1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 25, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families