UniProtKB - Q9D2E1 (TSSK3_MOUSE)
Protein
Testis-specific serine/threonine-protein kinase 3
Gene
Tssk3
Organism
Mus musculus (Mouse)
Status
Functioni
May be involved in a signaling pathway during male germ cell development or mature sperm function.
Caution
PubMed:10781952 has termed the gene 'STK22D' as it was then thought that there were two different closely related genes.Curated
Catalytic activityi
Cofactori
Mg2+By similarity
Activity regulationi
Activated by phosphorylation on Thr-168, potentially by autophosphorylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 134 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 16 – 24 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- intracellular signal transduction Source: GO_Central
- multicellular organism development Source: UniProtKB-KW
- protein phosphorylation Source: UniProtKB
- spermatogenesis Source: UniProtKB
- sperm capacitation Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation, Spermatogenesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Testis-specific serine/threonine-protein kinase 3 (EC:2.7.11.1)Short name: TSK-3 Short name: TSSK-3 Short name: Testis-specific kinase 3 Alternative name(s): Serine/threonine-protein kinase 22C |
Gene namesi | Name:Tssk3 Synonyms:Stk22c, Stk22d |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1929914, Tssk3 |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086771 | 1 – 268 | Testis-specific serine/threonine-protein kinase 3Add BLAST | 268 |
Post-translational modificationi
Autophosphorylated.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q9D2E1 |
PaxDbi | Q9D2E1 |
PeptideAtlasi | Q9D2E1 |
PRIDEi | Q9D2E1 |
PTM databases
iPTMneti | Q9D2E1 |
PhosphoSitePlusi | Q9D2E1 |
Expressioni
Tissue specificityi
Developmentally expressed in testicular germ cells. In adult testis, expression was detected in round and condensing spermatids, but not in meiotic pachytene spermatocytes. Not expressed in brain, ovary, kidney, liver or early embryonic cells.1 Publication
Developmental stagei
Expression begins 20-24 days after birth and is maximal in the adult. The pattern of expression suggests that STK22D is expressed postmeiotically.
Gene expression databases
Bgeei | ENSMUSG00000000411, Expressed in seminiferous tubule of testis and 46 other tissues |
Genevisiblei | Q9D2E1, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 208450, 2 interactors |
STRINGi | 10090.ENSMUSP00000000421 |
Miscellaneous databases
RNActi | Q9D2E1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 265 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000161490 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q9D2E1 |
OMAi | RYCHSCG |
OrthoDBi | 947464at2759 |
PhylomeDBi | Q9D2E1 |
TreeFami | TF352374 |
Family and domain databases
CDDi | cd14163, STKc_TSSK3-like, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR042709, TSSK3_STKc |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9D2E1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDFLLSNGY QLGKTIGEGT YSKVKEAFSK KHQRKVAIKI IDKMGGPEEF
60 70 80 90 100
IQRFLPRELQ IVRTLDHKNI IQVYEMLESA DGKIYLVMEL AEGGDVFDCV
110 120 130 140 150
LNGGPLPESR AKALFRQMVE AIRYCHGCGV AHRDLKCENA LLQGFNLKLT
160 170 180 190 200
DFGFAKVLPK SRRELSQTFC GSTAYAAPEV LQGIPHDSKK GDVWSMGVVL
210 220 230 240 250
YVMLCASLPF DDTDIPKMLW QQQKGVSFPT HLGISTECQD LLKRLLEPDM
260
ILRPSIEEVS WHPWLAST
Sequence cautioni
The sequence AAF72581 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF298901 mRNA Translation: AAK97209.1 AF201734 mRNA Translation: AAF72581.1 Frameshift. AK019840 mRNA Translation: BAB31876.1 BC048470 mRNA Translation: AAH48470.1 |
CCDSi | CCDS18693.1 |
RefSeqi | NP_536690.1, NM_080442.2 |
Genome annotation databases
Ensembli | ENSMUST00000000421; ENSMUSP00000000421; ENSMUSG00000000411 |
GeneIDi | 58864 |
KEGGi | mmu:58864 |
UCSCi | uc008uxc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF298901 mRNA Translation: AAK97209.1 AF201734 mRNA Translation: AAF72581.1 Frameshift. AK019840 mRNA Translation: BAB31876.1 BC048470 mRNA Translation: AAH48470.1 |
CCDSi | CCDS18693.1 |
RefSeqi | NP_536690.1, NM_080442.2 |
3D structure databases
SMRi | Q9D2E1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208450, 2 interactors |
STRINGi | 10090.ENSMUSP00000000421 |
PTM databases
iPTMneti | Q9D2E1 |
PhosphoSitePlusi | Q9D2E1 |
Proteomic databases
MaxQBi | Q9D2E1 |
PaxDbi | Q9D2E1 |
PeptideAtlasi | Q9D2E1 |
PRIDEi | Q9D2E1 |
Protocols and materials databases
Antibodypediai | 31305, 84 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000000421; ENSMUSP00000000421; ENSMUSG00000000411 |
GeneIDi | 58864 |
KEGGi | mmu:58864 |
UCSCi | uc008uxc.1, mouse |
Organism-specific databases
CTDi | 81629 |
MGIi | MGI:1929914, Tssk3 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
GeneTreei | ENSGT00940000161490 |
HOGENOMi | CLU_000288_63_0_1 |
InParanoidi | Q9D2E1 |
OMAi | RYCHSCG |
OrthoDBi | 947464at2759 |
PhylomeDBi | Q9D2E1 |
TreeFami | TF352374 |
Miscellaneous databases
BioGRID-ORCSi | 58864, 0 hits in 17 CRISPR screens |
PROi | PR:Q9D2E1 |
RNActi | Q9D2E1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000411, Expressed in seminiferous tubule of testis and 46 other tissues |
Genevisiblei | Q9D2E1, MM |
Family and domain databases
CDDi | cd14163, STKc_TSSK3-like, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR042709, TSSK3_STKc |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSSK3_MOUSE | |
Accessioni | Q9D2E1Primary (citable) accession number: Q9D2E1 Secondary accession number(s): Q9JL98 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 30, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index