UniProtKB - Q9D270 (ZDH21_MOUSE)
Protein
Palmitoyltransferase ZDHHC21
Gene
Zdhhc21
Organism
Mus musculus (Mouse)
Status
Functioni
Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed:19956733, PubMed:26715683, PubMed:27653213). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane. This affects rapid intracellular signaling by sex hormones via ERK and AKT kinases and the generation of cAMP, but does not affect that mediated by their nuclear receptor (By similarity). Palmitoylates FYN, regulates its localization in hair follicles and plays a key role in epidermal homeostasis and hair follicle differentiation (PubMed:19956733). Through the palmitoylation of PLCB1 and the regulation of PLCB1 downstream signaling may indirectly regulate the function of the endothelial barrier and the adhesion of leukocytes to the endothelium (PubMed:27653213). Has also a palmitoyltransferase activity toward ADRA1D, positively regulating its activity and expression and may thereby play a role in vascular contraction (PubMed:26715683). May also palmitoylate eNOS and LCK (PubMed:19956733).By similarity3 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 120 | S-palmitoyl cysteine intermediate2 Publications | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: MGI
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB
GO - Biological processi
- adrenergic receptor signaling pathway Source: UniProtKB
- hair follicle development Source: MGI
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- protein palmitoylation Source: MGI
- protein targeting to membrane Source: GO_Central
- regulation of establishment of endothelial barrier Source: UniProtKB
- regulation of leukocyte adhesion to arterial endothelial cell Source: UniProtKB
- regulation of vascular associated smooth muscle contraction Source: UniProtKB
- sebaceous gland development Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-MMU-203615, eNOS activation R-MMU-9009391, Extra-nuclear estrogen signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC211 Publication (EC:2.3.1.2253 Publications)Alternative name(s): DHHC domain-containing cysteine-rich protein 211 Publication Short name: DHHC211 Publication GABA-A receptor-associated membrane protein 31 Publication Zinc finger DHHC domain-containing protein 21Imported |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915518, Zdhhc21 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
- cis-Golgi network membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 44 | ExtracellularCurated | 7 | |
Transmembranei | 45 – 65 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 66 – 133 | CytoplasmicCuratedAdd BLAST | 68 | |
Transmembranei | 134 – 154 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 155 – 185 | ExtracellularCuratedAdd BLAST | 31 | |
Transmembranei | 186 – 206 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 207 – 265 | CytoplasmicCuratedAdd BLAST | 59 |
Keywords - Cellular componenti
Cell membrane, Golgi apparatus, MembranePathology & Biotechi
Involvement in diseasei
Defects in zdhhc21 are the cause of the depilated (dep) phenotype. It is a recessive phenotype characterized by variable hair loss, with thinner and shorter hairs remaining in a greasy coat which is due to a defect in the epidermis, rather than the dermis (PubMed:19956733). Depilated (dep) mutant mice are protected against endothelial dysfunction in systemic inflammatory response syndrome (PubMed:27653213). They are also tachycardic and hypotensive (PubMed:26715683).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 91 | L → F: No effect on protein-cysteine S-palmitoyltransferase activity. No effect on localization to the cis-Golgi network. 1 Publication | 1 | |
Mutagenesisi | 95 | C → S: Loss of protein-cysteine S-palmitoyltransferase activity. Decreased localization to the cis-Golgi network. Relocalized to the endoplasmic reticulum. 1 Publication | 1 | |
Mutagenesisi | 106 | C → S: Loss of protein-cysteine S-palmitoyltransferase activity. Decreased localization to the cis-Golgi network. Relocalized to the endoplasmic reticulum. 1 Publication | 1 | |
Mutagenesisi | 120 | C → S: Loss of protein-cysteine S-palmitoyltransferase activity. Decreased localization to the cis-Golgi network. Relocalized to the endoplasmic reticulum. 2 Publications | 1 | |
Mutagenesisi | 233 | Missing in depilated (dep) mutant mice. Loss of protein-cysteine S-palmitoyltransferase activity. Relocalized to the endoplasmic reticulum. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212909 | 1 – 265 | Palmitoyltransferase ZDHHC21Add BLAST | 265 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
EPDi | Q9D270 |
MaxQBi | Q9D270 |
PaxDbi | Q9D270 |
PeptideAtlasi | Q9D270 |
PRIDEi | Q9D270 |
PTM databases
PhosphoSitePlusi | Q9D270 |
SwissPalmi | Q9D270 |
Expressioni
Tissue specificityi
Widely expressed (PubMed:26715683). Expressed in Henle's layer within the hair bulb and the hair shaft cuticle (at protein level) (PubMed:19956733). Expression is limited to the post-mitotic lineages of inner root sheath (IRS) and cuticle (PubMed:19956733).2 Publications
Developmental stagei
In the developing skin, expression is not be detected prior to hair follicle induction (13 dpc). Expression is initially detected in the inner root sheath (IRS) of developing vibrissae follicles at 16 dpc and later in the developing IRS of E18.5 pelage follicles.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000028403, Expressed in utricle of membranous labyrinth and 256 other tissues |
ExpressionAtlasi | Q9D270, baseline and differential |
Genevisiblei | Q9D270, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000030110 |
Miscellaneous databases
RNActi | Q9D270, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 90 – 140 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Belongs to the DHHC palmitoyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000158006 |
HOGENOMi | CLU_048061_3_0_1 |
InParanoidi | Q9D270 |
OMAi | KRSWQWA |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q9D270 |
TreeFami | TF319798 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9D270-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLRIHFVVD PHGWCCMGLI VFVWLYNIVI IPKIVLFPHY EEGHIPGILI
60 70 80 90 100
IIFYGISIFC LVALVRASLT DPGRLPENPK IPHAERELWE LCNKCNLMRP
110 120 130 140 150
KRSHHCSRCG HCVRRMDHHC PWINNCVGED NHWLFLQLCF YTELLTCYAL
160 170 180 190 200
MFSFCHYYYF LPLKKRNLDL FVVRHELAIM RLAAFMGITM LVGITGLFYT
210 220 230 240 250
QLIGIITDTT SIEKMSNCCE EISRPRKPWQ QTFSEVFGTR WKILWFIPFR
260
QRQPLRVPYH FANHV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UXC1 | G3UXC1_MOUSE | Palmitoyltransferase | Zdhhc21 | 133 | Annotation score: | ||
Q5SRN8 | Q5SRN8_MOUSE | Palmitoyltransferase ZDHHC21 | Zdhhc21 | 109 | Annotation score: | ||
Q5SRP0 | Q5SRP0_MOUSE | Palmitoyltransferase ZDHHC21 | Zdhhc21 | 26 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 67 | A → T in AAO27361 (PubMed:15229235).Curated | 1 | |
Sequence conflicti | 67 | A → T in BAB32059 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166674 mRNA Translation: AAO27361.1 AK020293 mRNA Translation: BAB32059.1 AL671005 Genomic DNA No translation available. BC043110 mRNA Translation: AAH43110.1 |
CCDSi | CCDS18294.1 |
RefSeqi | NP_080923.2, NM_026647.3 XP_006538279.1, XM_006538216.3 XP_011248395.1, XM_011250093.2 XP_011248396.1, XM_011250094.2 XP_017175861.1, XM_017320372.1 XP_017175862.1, XM_017320373.1 |
Genome annotation databases
Ensembli | ENSMUST00000030110; ENSMUSP00000030110; ENSMUSG00000028403 ENSMUST00000107239; ENSMUSP00000102859; ENSMUSG00000028403 |
GeneIDi | 68268 |
KEGGi | mmu:68268 |
UCSCi | uc008tkj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY166674 mRNA Translation: AAO27361.1 AK020293 mRNA Translation: BAB32059.1 AL671005 Genomic DNA No translation available. BC043110 mRNA Translation: AAH43110.1 |
CCDSi | CCDS18294.1 |
RefSeqi | NP_080923.2, NM_026647.3 XP_006538279.1, XM_006538216.3 XP_011248395.1, XM_011250093.2 XP_011248396.1, XM_011250094.2 XP_017175861.1, XM_017320372.1 XP_017175862.1, XM_017320373.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000030110 |
PTM databases
PhosphoSitePlusi | Q9D270 |
SwissPalmi | Q9D270 |
Proteomic databases
EPDi | Q9D270 |
MaxQBi | Q9D270 |
PaxDbi | Q9D270 |
PeptideAtlasi | Q9D270 |
PRIDEi | Q9D270 |
Protocols and materials databases
Antibodypediai | 42748, 109 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030110; ENSMUSP00000030110; ENSMUSG00000028403 ENSMUST00000107239; ENSMUSP00000102859; ENSMUSG00000028403 |
GeneIDi | 68268 |
KEGGi | mmu:68268 |
UCSCi | uc008tkj.2, mouse |
Organism-specific databases
CTDi | 340481 |
MGIi | MGI:1915518, Zdhhc21 |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000158006 |
HOGENOMi | CLU_048061_3_0_1 |
InParanoidi | Q9D270 |
OMAi | KRSWQWA |
OrthoDBi | 1491968at2759 |
PhylomeDBi | Q9D270 |
TreeFami | TF319798 |
Enzyme and pathway databases
Reactomei | R-MMU-203615, eNOS activation R-MMU-9009391, Extra-nuclear estrogen signaling |
Miscellaneous databases
BioGRID-ORCSi | 68268, 1 hit in 17 CRISPR screens |
ChiTaRSi | Zdhhc21, mouse |
PROi | PR:Q9D270 |
RNActi | Q9D270, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028403, Expressed in utricle of membranous labyrinth and 256 other tissues |
ExpressionAtlasi | Q9D270, baseline and differential |
Genevisiblei | Q9D270, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDH21_MOUSE | |
Accessioni | Q9D270Primary (citable) accession number: Q9D270 Secondary accession number(s): Q6EMK1, Q80XQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | November 22, 2005 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families