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Protein

Ribonuclease K6

Gene

Rnase6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:15693621). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:15693621, PubMed:25075772). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (By similarity). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (By similarity).By similarity2 Publications

Kineticsi

  1. KM=2.1 µM for tRNA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei41Proton acceptorBy similarity1
    Sitei62Facilitates cleavage of polynucleotide substratesBy similarity1
    Sitei64Critical for catalytic activityBy similarity1
    Binding sitei89SubstrateBy similarity1
    Active sitei148Proton donorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAntibiotic, Antimicrobial, Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease K6 (EC:3.1.27.-1 Publication)
    Short name:
    RNase K6
    Gene namesi
    Name:Rnase6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Unplaced

    Organism-specific databases

    MGIiMGI:1925666 Rnase6

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Lysosome, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 27Sequence analysisAdd BLAST27
    ChainiPRO_000003090028 – 153Ribonuclease K6Add BLAST126

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi49 ↔ 107By similarity
    Glycosylationi58N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi63 ↔ 117By similarity
    Disulfide bondi81 ↔ 132By similarity
    Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi88 ↔ 95By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9D244
    PRIDEiQ9D244

    Expressioni

    Tissue specificityi

    Highly expressed in spleen (at protein level) (PubMed:15693621, PubMed:25075772). Has little or no expression in healthy kidneys (at protein level) (PubMed:25075772). Detected at high levels in infected kidneys (at protein level) (PubMed:25075772). Expressed at low levels in bladder (PubMed:15693621, PubMed:25075772). Also detected in skeletal muscle, heart and bone marrow (PubMed:15693621).1 Publication

    Inductioni

    Up-regulated in bone marrow derived macrophages in response to the uropathogenic E.coli strain CFT073.1 Publication

    Gene expression databases

    CleanExiMM_RNASE6

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with bacterial lipopolysaccharide (LPS).By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000093613

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D244
    SMRiQ9D244
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni64 – 68Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the pancreatic ribonuclease family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IYEW Eukaryota
    ENOG4111D8Q LUCA
    HOGENOMiHOG000276882
    HOVERGENiHBG008396
    InParanoidiQ9D244
    KOiK01172
    PhylomeDBiQ9D244

    Family and domain databases

    Gene3Di3.10.130.10, 1 hit
    InterProiView protein in InterPro
    IPR001427 RNaseA
    IPR036816 RNaseA-like_dom_sf
    IPR023411 RNaseA_AS
    IPR023412 RNaseA_domain
    PANTHERiPTHR11437 PTHR11437, 1 hit
    PfamiView protein in Pfam
    PF00074 RnaseA, 1 hit
    PRINTSiPR00794 RIBONUCLEASE
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD000535 RNaseA, 1 hit
    SMARTiView protein in SMART
    SM00092 RNAse_Pc, 1 hit
    SUPFAMiSSF54076 SSF54076, 1 hit
    PROSITEiView protein in PROSITE
    PS00127 RNASE_PANCREATIC, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

    Q9D244-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVVDLPRYLP LLLLLELWEP MYLLCSQPKG LSRAHWFEIQ HIQTSRQPCN
    60 70 80 90 100
    TAMRGVNNYT QHCKQINTFL HESFQNVAAT CSLHNITCKN GRKNCHESAE
    110 120 130 140 150
    PVKMTDCSHT GGAYPNCRYS SDKQYKFFIV ACEHPKKEDP PYQLVPVHLD

    KIV
    Length:153
    Mass (Da):17,675
    Last modified:July 5, 2005 - v2
    Checksum:i84A6C788AEE7CA05
    GO

    Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    Q3V3L3Q3V3L3_MOUSE
    Ribonuclease K6
    Rnase6
    153Annotation score:
    A0A2I3BS15A0A2I3BS15_MOUSE
    Ribonuclease K6
    Rnase6
    33Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti42I → V in BAB32143 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY545655 mRNA Translation: AAS48640.1
    AK020595 mRNA Translation: BAB32143.1
    BC094892 mRNA Translation: AAH94892.1
    BC132632 mRNA Translation: AAI32633.1
    CCDSiCCDS27037.1
    RefSeqiNP_084374.2, NM_030098.2
    XP_006519761.1, XM_006519698.2
    UniGeneiMm.103835

    Genome annotation databases

    GeneIDi78416
    KEGGimmu:78416

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY545655 mRNA Translation: AAS48640.1
    AK020595 mRNA Translation: BAB32143.1
    BC094892 mRNA Translation: AAH94892.1
    BC132632 mRNA Translation: AAI32633.1
    CCDSiCCDS27037.1
    RefSeqiNP_084374.2, NM_030098.2
    XP_006519761.1, XM_006519698.2
    UniGeneiMm.103835

    3D structure databases

    ProteinModelPortaliQ9D244
    SMRiQ9D244
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000093613

    Proteomic databases

    PaxDbiQ9D244
    PRIDEiQ9D244

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi78416
    KEGGimmu:78416

    Organism-specific databases

    CTDi6039
    MGIiMGI:1925666 Rnase6

    Phylogenomic databases

    eggNOGiENOG410IYEW Eukaryota
    ENOG4111D8Q LUCA
    HOGENOMiHOG000276882
    HOVERGENiHBG008396
    InParanoidiQ9D244
    KOiK01172
    PhylomeDBiQ9D244

    Miscellaneous databases

    PROiPR:Q9D244
    SOURCEiSearch...

    Gene expression databases

    CleanExiMM_RNASE6

    Family and domain databases

    Gene3Di3.10.130.10, 1 hit
    InterProiView protein in InterPro
    IPR001427 RNaseA
    IPR036816 RNaseA-like_dom_sf
    IPR023411 RNaseA_AS
    IPR023412 RNaseA_domain
    PANTHERiPTHR11437 PTHR11437, 1 hit
    PfamiView protein in Pfam
    PF00074 RnaseA, 1 hit
    PRINTSiPR00794 RIBONUCLEASE
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD000535 RNaseA, 1 hit
    SMARTiView protein in SMART
    SM00092 RNAse_Pc, 1 hit
    SUPFAMiSSF54076 SSF54076, 1 hit
    PROSITEiView protein in PROSITE
    PS00127 RNASE_PANCREATIC, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRNAS6_MOUSE
    AccessioniPrimary (citable) accession number: Q9D244
    Secondary accession number(s): A2RTT6, Q6QDX5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 19, 2001
    Last sequence update: July 5, 2005
    Last modified: July 18, 2018
    This is version 111 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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