UniProtKB - Q9D1Z3 (ACKMT_MOUSE)
Protein
ATP synthase subunit C lysine N-methyltransferase
Gene
Atpsckmt
Organism
Mus musculus (Mouse)
Status
Functioni
Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration (PubMed:29444090, PubMed:30530489). Promotes chronic pain (PubMed:29444090). Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia (PubMed:29444090).2 Publications
Catalytic activityi
- L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H+ + N6,N6,N6-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine1 PublicationThis reaction proceeds in the forward1 Publication direction.
GO - Molecular functioni
- protein-lysine N-methyltransferase activity Source: UniProtKB
GO - Biological processi
- peptidyl-lysine methylation Source: UniProtKB
- peptidyl-lysine trimethylation Source: UniProtKB
- positive regulation of proton-transporting ATP synthase activity, rotational mechanism Source: UniProtKB
- positive regulation of sensory perception of pain Source: UniProtKB
- regulation of mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: ATP synthase subunit C lysine N-methyltransferaseCurated (EC:2.1.1.-1 Publication)Alternative name(s): Protein N-lysine methyltransferase FAM173BCurated Short name: mFam173b1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915323, Atpsckmt |
Subcellular locationi
Mitochondrion
- Mitochondrion membrane By similarity; Single-pass membrane protein Sequence analysis
Note: Localizes to mitochondrial cristae.By similarity
Mitochondrion
- mitochondrial crista Source: UniProtKB
- mitochondrion Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 34 – 54 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000321537 | 1 – 247 | ATP synthase subunit C lysine N-methyltransferaseAdd BLAST | 247 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9D1Z3 |
MaxQBi | Q9D1Z3 |
PaxDbi | Q9D1Z3 |
PeptideAtlasi | Q9D1Z3 |
PRIDEi | Q9D1Z3 |
ProteomicsDBi | 275974 |
PTM databases
PhosphoSitePlusi | Q9D1Z3 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Inductioni
Expression is up-regulated in dorsal root ganglia (DRG) during chronic inflammatory pain.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039065, Expressed in ear vesicle and 217 other tissues |
ExpressionAtlasi | Q9D1Z3, baseline and differential |
Genevisiblei | Q9D1Z3, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 212640, 1 interactor |
STRINGi | 10090.ENSMUSP00000039094 |
Miscellaneous databases
RNActi | Q9D1Z3, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 85 | Required for mitochondrial locationBy similarityAdd BLAST | 35 |
Domaini
Contains an atypical, non-cleavable mitochondrial targeting sequence responsible for its localization to mitochondria.By similarity
Sequence similaritiesi
Belongs to the ANT/ATPSC lysine N-methyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4058, Eukaryota |
GeneTreei | ENSGT00390000014771 |
HOGENOMi | CLU_068443_4_0_1 |
InParanoidi | Q9D1Z3 |
OMAi | GVNTVWF |
OrthoDBi | 1605787at2759 |
PhylomeDBi | Q9D1Z3 |
TreeFami | TF314984 |
Family and domain databases
InterProi | View protein in InterPro IPR026170, FAM173A/B IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13610, PTHR13610, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9D1Z3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERVGTPEEE RQAGPVLPTS LESDSSKRTS WGFLITGVVG GALLTVYAVA
60 70 80 90 100
TPFITPALRK VCLPFVPATS KQVENVVRML RHRRGPLVDI GSGDGRIVIA
110 120 130 140 150
AAKEGFPAVG YELNPWLVWY SRYRAWRAGV HGSAKFYISD LWKVTFAQYS
160 170 180 190 200
NVVIFGVPQM MPQLEKKLEL ELEDGARVIA CRFPFPRWTP DHTTGEGIDT
210 220 230 240
VWAYDMSAQR GRGGRPNQEW VGQKNLSETA GLQASSSETR SKLLDVE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CXY7 | E0CXY7_MOUSE | ATP synthase subunit C lysine N-met... | Atpsckmt Fam173b | 169 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 229 | T → I in AAH30179 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK020868 mRNA Translation: BAB32234.1 BC030179 mRNA Translation: AAH30179.1 |
CCDSi | CCDS27412.1 |
RefSeqi | NP_080822.1, NM_026546.3 |
Genome annotation databases
Ensembli | ENSMUST00000042702; ENSMUSP00000039094; ENSMUSG00000039065 |
GeneIDi | 68073 |
KEGGi | mmu:68073 |
UCSCi | uc007vkk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK020868 mRNA Translation: BAB32234.1 BC030179 mRNA Translation: AAH30179.1 |
CCDSi | CCDS27412.1 |
RefSeqi | NP_080822.1, NM_026546.3 |
3D structure databases
SMRi | Q9D1Z3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212640, 1 interactor |
STRINGi | 10090.ENSMUSP00000039094 |
PTM databases
PhosphoSitePlusi | Q9D1Z3 |
Proteomic databases
EPDi | Q9D1Z3 |
MaxQBi | Q9D1Z3 |
PaxDbi | Q9D1Z3 |
PeptideAtlasi | Q9D1Z3 |
PRIDEi | Q9D1Z3 |
ProteomicsDBi | 275974 |
Protocols and materials databases
Antibodypediai | 22448, 68 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042702; ENSMUSP00000039094; ENSMUSG00000039065 |
GeneIDi | 68073 |
KEGGi | mmu:68073 |
UCSCi | uc007vkk.1, mouse |
Organism-specific databases
CTDi | 134145 |
MGIi | MGI:1915323, Atpsckmt |
Phylogenomic databases
eggNOGi | KOG4058, Eukaryota |
GeneTreei | ENSGT00390000014771 |
HOGENOMi | CLU_068443_4_0_1 |
InParanoidi | Q9D1Z3 |
OMAi | GVNTVWF |
OrthoDBi | 1605787at2759 |
PhylomeDBi | Q9D1Z3 |
TreeFami | TF314984 |
Miscellaneous databases
BioGRID-ORCSi | 68073, 4 hits in 52 CRISPR screens |
ChiTaRSi | Fam173b, mouse |
PROi | PR:Q9D1Z3 |
RNActi | Q9D1Z3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039065, Expressed in ear vesicle and 217 other tissues |
ExpressionAtlasi | Q9D1Z3, baseline and differential |
Genevisiblei | Q9D1Z3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR026170, FAM173A/B IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13610, PTHR13610, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACKMT_MOUSE | |
Accessioni | Q9D1Z3Primary (citable) accession number: Q9D1Z3 Secondary accession number(s): Q8K2S1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families