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Protein

Golgi to ER traffic protein 4 homolog

Gene

Get4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation. The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum. Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome. Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum. The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 4 homolog
Gene namesi
Name:Get4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914854 Get4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002281052 – 327Golgi to ER traffic protein 4 homologAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

Ubiquitinated by RNF12, leading to proteasomal degradation. When unassembled from BAG6; ubiquitinylation is modulated by BAG6 quality control role and effectuated by RNF126.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ9D1H7
PaxDbiQ9D1H7
PeptideAtlasiQ9D1H7
PRIDEiQ9D1H7

PTM databases

iPTMnetiQ9D1H7
PhosphoSitePlusiQ9D1H7

Expressioni

Gene expression databases

BgeeiENSMUSG00000025858 Expressed in 28 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_1110007L15RIK
ExpressionAtlasiQ9D1H7 baseline and differential
GenevisibleiQ9D1H7 MM

Interactioni

Subunit structurei

Component of the BAG6/BAT3 complex, at least composed of BAG6, UBL4A and GET4/TRC35. Interacts with BAG6; the interaction is direct and localizes BAG6 in the cytosol.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212305, 24 interactors
ComplexPortaliCPX-133 BAT3 complex
IntActiQ9D1H7, 23 interactors
STRINGi10090.ENSMUSP00000026976

Structurei

3D structure databases

ProteinModelPortaliQ9D1H7
SMRiQ9D1H7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 271Interacts with BAG6By similarityAdd BLAST77

Sequence similaritiesi

Belongs to the GET4 family.Curated

Phylogenomic databases

eggNOGiKOG3024 Eukaryota
ENOG410XRWE LUCA
GeneTreeiENSGT00390000015750
HOGENOMiHOG000264232
HOVERGENiHBG059877
InParanoidiQ9D1H7
OMAiRYMSQGK
OrthoDBiEOG091G0DTD
PhylomeDBiQ9D1H7
TreeFamiTF315163

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011990 TPR-like_helical_dom_sf
IPR007317 UPF0363
PANTHERiPTHR12875 PTHR12875, 1 hit
PfamiView protein in Pfam
PF04190 DUF410, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9D1H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAAAMAEQ EGARNGARNR GGVQRVEGKL RASVEKGDYY EAHQMYRTLF
60 70 80 90 100
FRYMSQSKHA EARELMYSGA LLFFSHGQQN SAADLSMLVL ESLEKAEVDV
110 120 130 140 150
ADELLENLAK VFSLMDPNSP ERVAFVSRAL KWSSGGSGKL GHPRLHQLLA
160 170 180 190 200
LTLWKEQNYC ESRYHFLHSS DGEGCANMLV EYSTARGFRS EVDMFVAQAV
210 220 230 240 250
LQFLCLKNKN SALVVFTTYT QKHPSIEDGP PFVQPLLNFI WFLLLAVDGG
260 270 280 290 300
KLAVFTVLCE QYQPSLRRDP MYNEYLDRIG QLFFGVPPKQ TSSYGGLLGN
310 320
LLSSLMGSSE QEEGEESQDD SSPIELD
Note: No experimental confirmation available.
Length:327
Mass (Da):36,525
Last modified:July 5, 2004 - v2
Checksum:i04B8DCC8E75FB022
GO
Isoform 2 (identifier: Q9D1H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Note: No experimental confirmation available.
Show »
Length:274
Mass (Da):30,560
Checksum:i185B7840B12D0698
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z7S0D3Z7S0_MOUSE
Golgi to ER traffic protein 4 homol...
Get4
197Annotation score:
D3Z4J5D3Z4J5_MOUSE
Golgi to ER traffic protein 4 homol...
Get4
246Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0176531 – 53Missing in isoform 2. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003551 mRNA Translation: BAB22851.2
BC019557 mRNA Translation: AAH19557.1
CCDSiCCDS19805.1 [Q9D1H7-1]
CCDS51682.1 [Q9D1H7-2]
RefSeqiNP_001156788.1, NM_001163316.1 [Q9D1H7-2]
NP_080545.2, NM_026269.2 [Q9D1H7-1]
UniGeneiMm.270137

Genome annotation databases

EnsembliENSMUST00000026976; ENSMUSP00000026976; ENSMUSG00000025858 [Q9D1H7-1]
ENSMUST00000110878; ENSMUSP00000106502; ENSMUSG00000025858 [Q9D1H7-2]
GeneIDi67604
KEGGimmu:67604
UCSCiuc009agg.2 mouse [Q9D1H7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003551 mRNA Translation: BAB22851.2
BC019557 mRNA Translation: AAH19557.1
CCDSiCCDS19805.1 [Q9D1H7-1]
CCDS51682.1 [Q9D1H7-2]
RefSeqiNP_001156788.1, NM_001163316.1 [Q9D1H7-2]
NP_080545.2, NM_026269.2 [Q9D1H7-1]
UniGeneiMm.270137

3D structure databases

ProteinModelPortaliQ9D1H7
SMRiQ9D1H7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212305, 24 interactors
ComplexPortaliCPX-133 BAT3 complex
IntActiQ9D1H7, 23 interactors
STRINGi10090.ENSMUSP00000026976

PTM databases

iPTMnetiQ9D1H7
PhosphoSitePlusiQ9D1H7

Proteomic databases

EPDiQ9D1H7
PaxDbiQ9D1H7
PeptideAtlasiQ9D1H7
PRIDEiQ9D1H7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026976; ENSMUSP00000026976; ENSMUSG00000025858 [Q9D1H7-1]
ENSMUST00000110878; ENSMUSP00000106502; ENSMUSG00000025858 [Q9D1H7-2]
GeneIDi67604
KEGGimmu:67604
UCSCiuc009agg.2 mouse [Q9D1H7-1]

Organism-specific databases

CTDi51608
MGIiMGI:1914854 Get4

Phylogenomic databases

eggNOGiKOG3024 Eukaryota
ENOG410XRWE LUCA
GeneTreeiENSGT00390000015750
HOGENOMiHOG000264232
HOVERGENiHBG059877
InParanoidiQ9D1H7
OMAiRYMSQGK
OrthoDBiEOG091G0DTD
PhylomeDBiQ9D1H7
TreeFamiTF315163

Miscellaneous databases

ChiTaRSiGet4 mouse
PROiPR:Q9D1H7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025858 Expressed in 28 organ(s), highest expression level in skeletal muscle tissue
CleanExiMM_1110007L15RIK
ExpressionAtlasiQ9D1H7 baseline and differential
GenevisibleiQ9D1H7 MM

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR011990 TPR-like_helical_dom_sf
IPR007317 UPF0363
PANTHERiPTHR12875 PTHR12875, 1 hit
PfamiView protein in Pfam
PF04190 DUF410, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGET4_MOUSE
AccessioniPrimary (citable) accession number: Q9D1H7
Secondary accession number(s): Q78J86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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