Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline-rich AKT1 substrate 1

Gene

Akt1s1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.2 Publications

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-165159 mTOR signalling
R-MMU-166208 mTORC1-mediated signalling
R-MMU-198323 AKT phosphorylates targets in the cytosol
R-MMU-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich AKT1 substrate 1
Short name:
Proline-rich AKT substrate
Gene namesi
Name:Akt1s1Imported
Synonyms:Pras1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914855 Akt1s1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002534471 – 257Proline-rich AKT1 substrate 1Add BLAST257

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51Omega-N-methylarginineCombined sources1
Modified residuei88PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei213PhosphoserineCombined sources1
Modified residuei247PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated by AKT1. Phosphorylation at Thr-247 by DYRK3 relieves inhibitory function on mTORC1.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9D1F4
MaxQBiQ9D1F4
PaxDbiQ9D1F4
PRIDEiQ9D1F4

PTM databases

iPTMnetiQ9D1F4
PhosphoSitePlusiQ9D1F4

Expressioni

Gene expression databases

BgeeiENSMUSG00000011096 Expressed in 251 organ(s), highest expression level in ear
CleanExiMM_AKT1S1
ExpressionAtlasiQ9D1F4 baseline and differential
GenevisibleiQ9D1F4 MM

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins and with phosphorylated AKT1 following transient focal cerebral ischemia in vivo.1 Publication

Protein-protein interaction databases

IntActiQ9D1F4, 1 interactor
MINTiQ9D1F4
STRINGi10090.ENSMUSP00000049764

Structurei

3D structure databases

ProteinModelPortaliQ9D1F4
SMRiQ9D1F4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi35 – 43Poly-ProSequence analysis9
Compositional biasi77 – 97Pro-richSequence analysisAdd BLAST21

Phylogenomic databases

eggNOGiENOG410IXEE Eukaryota
ENOG41121RI LUCA
GeneTreeiENSGT00390000017397
HOGENOMiHOG000236322
HOVERGENiHBG059465
InParanoidiQ9D1F4
KOiK16184
PhylomeDBiQ9D1F4

Family and domain databases

InterProiView protein in InterPro
IPR026682 AKT1S1
PANTHERiPTHR21844 PTHR21844, 2 hits
PfamiView protein in Pfam
PF15798 PRAS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

Q9D1F4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGRPEELW EAVVGAAERF QARTGTELVL LTAAPPPPPR PGPCAYAAHG
60 70 80 90 100
RGALAEAARR CLHDIAQAHR AATATRPPGP PPAPQPPSPA PSPPPRPALA
110 120 130 140 150
REDEEEDEDE PTETETSGER LGGSDNGGLF MMDEDATLQD LPPFCESDPE
160 170 180 190 200
STDDGSLSEE TPAGPTACPQ PPATALPTQQ YAKSLPVSVP VWAFKEKRTE
210 220 230 240 250
ARSSDEENGP PSSPDLDRIA ASMRALVLRE AEDTQVFGDL PRPRLNTSDF

QKLKRKY
Length:257
Mass (Da):27,483
Last modified:June 1, 2001 - v1
Checksum:i4BFFC8630DBBA5EC
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QKI5E9QKI5_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
283Annotation score:
E9QKI4E9QKI4_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
328Annotation score:
D3YWZ1D3YWZ1_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
193Annotation score:
F6R8S6F6R8S6_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
247Annotation score:
A0A140LHA0A0A140LHA0_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
111Annotation score:
D3Z4W3D3Z4W3_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
95Annotation score:
D3Z4R0D3Z4R0_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
123Annotation score:
A0A140LJJ3A0A140LJJ3_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
57Annotation score:
A0A140LHN7A0A140LHN7_MOUSE
Proline-rich AKT1 substrate 1
Akt1s1
14Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003638 mRNA Translation: BAB22905.1
AK141771 mRNA Translation: BAE24829.1
AK154110 mRNA Translation: BAE32383.1
BC132372 mRNA Translation: AAI32373.1
BC132374 mRNA Translation: AAI32375.1
CCDSiCCDS21218.1
RefSeqiNP_001240849.1, NM_001253920.1
NP_001277623.1, NM_001290694.1
NP_001277624.1, NM_001290695.1
NP_080546.1, NM_026270.4
XP_006541182.1, XM_006541119.3
XP_006541183.1, XM_006541120.3
XP_006541184.1, XM_006541121.3
XP_006541185.1, XM_006541122.2
UniGeneiMm.148007
Mm.491121

Genome annotation databases

EnsembliENSMUST00000054343; ENSMUSP00000049764; ENSMUSG00000011096
ENSMUST00000107880; ENSMUSP00000103512; ENSMUSG00000011096
GeneIDi67605
KEGGimmu:67605
UCSCiuc009grd.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003638 mRNA Translation: BAB22905.1
AK141771 mRNA Translation: BAE24829.1
AK154110 mRNA Translation: BAE32383.1
BC132372 mRNA Translation: AAI32373.1
BC132374 mRNA Translation: AAI32375.1
CCDSiCCDS21218.1
RefSeqiNP_001240849.1, NM_001253920.1
NP_001277623.1, NM_001290694.1
NP_001277624.1, NM_001290695.1
NP_080546.1, NM_026270.4
XP_006541182.1, XM_006541119.3
XP_006541183.1, XM_006541120.3
XP_006541184.1, XM_006541121.3
XP_006541185.1, XM_006541122.2
UniGeneiMm.148007
Mm.491121

3D structure databases

ProteinModelPortaliQ9D1F4
SMRiQ9D1F4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9D1F4, 1 interactor
MINTiQ9D1F4
STRINGi10090.ENSMUSP00000049764

PTM databases

iPTMnetiQ9D1F4
PhosphoSitePlusiQ9D1F4

Proteomic databases

EPDiQ9D1F4
MaxQBiQ9D1F4
PaxDbiQ9D1F4
PRIDEiQ9D1F4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054343; ENSMUSP00000049764; ENSMUSG00000011096
ENSMUST00000107880; ENSMUSP00000103512; ENSMUSG00000011096
GeneIDi67605
KEGGimmu:67605
UCSCiuc009grd.2 mouse

Organism-specific databases

CTDi84335
MGIiMGI:1914855 Akt1s1

Phylogenomic databases

eggNOGiENOG410IXEE Eukaryota
ENOG41121RI LUCA
GeneTreeiENSGT00390000017397
HOGENOMiHOG000236322
HOVERGENiHBG059465
InParanoidiQ9D1F4
KOiK16184
PhylomeDBiQ9D1F4

Enzyme and pathway databases

ReactomeiR-MMU-165159 mTOR signalling
R-MMU-166208 mTORC1-mediated signalling
R-MMU-198323 AKT phosphorylates targets in the cytosol
R-MMU-3371571 HSF1-dependent transactivation

Miscellaneous databases

ChiTaRSiAkt1s1 mouse
PROiPR:Q9D1F4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000011096 Expressed in 251 organ(s), highest expression level in ear
CleanExiMM_AKT1S1
ExpressionAtlasiQ9D1F4 baseline and differential
GenevisibleiQ9D1F4 MM

Family and domain databases

InterProiView protein in InterPro
IPR026682 AKT1S1
PANTHERiPTHR21844 PTHR21844, 2 hits
PfamiView protein in Pfam
PF15798 PRAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAKTS1_MOUSE
AccessioniPrimary (citable) accession number: Q9D1F4
Secondary accession number(s): A2RT52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again