UniProtKB - Q9D1E6 (TBCB_MOUSE)
Tubulin-folding cofactor B
Tbcb
Functioni
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (By similarity).
Involved in regulation of tubulin heterodimer dissociation (PubMed:17184771).
May function as a negative regulator of axonal growth (PubMed:17217416).
By similarity2 PublicationsGO - Biological processi
- cell differentiation Source: UniProtKB-KW
- nervous system development Source: UniProtKB-KW
Keywordsi
Molecular function | Chaperone, Developmental protein |
Biological process | Differentiation, Neurogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin-folding cofactor BAlternative name(s): Cytoskeleton-associated protein 1 Cytoskeleton-associated protein CKAPI Tubulin-specific chaperone B |
Gene namesi | Name:Tbcb Synonyms:Ckap1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913661, Tbcb |
VEuPathDBi | HostDB:ENSMUSG00000006095 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.1 Publication
Cytoskeleton
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083535 | 1 – 244 | Tubulin-folding cofactor BAdd BLAST | 244 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 65 | Phosphoserine; by PAK1By similarity | 1 | |
Modified residuei | 98 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 128 | Phosphoserine; by PAK1By similarity | 1 | |
Modified residuei | 219 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9D1E6 |
jPOSTi | Q9D1E6 |
MaxQBi | Q9D1E6 |
PaxDbi | Q9D1E6 |
PeptideAtlasi | Q9D1E6 |
PRIDEi | Q9D1E6 |
ProteomicsDBi | 263133 |
PTM databases
iPTMneti | Q9D1E6 |
PhosphoSitePlusi | Q9D1E6 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000006095, Expressed in facial nucleus and 305 other tissues |
Genevisiblei | Q9D1E6, MM |
Interactioni
Subunit structurei
Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (By similarity). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (PubMed:17184771).
Interacts with GAN.
Interacts with DCTN1 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 211455, 18 interactors |
IntActi | Q9D1E6, 2 interactors |
MINTi | Q9D1E6 |
STRINGi | 10090.ENSMUSP00000006254 |
Miscellaneous databases
RNActi | Q9D1E6, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9D1E6 |
BMRBi | Q9D1E6 |
SMRi | Q9D1E6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9D1E6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 225 | CAP-GlyPROSITE-ProRule annotationAdd BLAST | 43 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3206, Eukaryota |
GeneTreei | ENSGT00940000156119 |
HOGENOMi | CLU_067577_0_0_1 |
InParanoidi | Q9D1E6 |
OMAi | IGVKYDE |
OrthoDBi | 1550378at2759 |
PhylomeDBi | Q9D1E6 |
TreeFami | TF313444 |
Family and domain databases
CDDi | cd01789, Ubl_TBCB, 1 hit |
Gene3Di | 2.30.30.190, 1 hit |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR045172, TBCB_N_Ubl IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEVTGISAPT VMVFISSSLN SFRSEKRYSR SLTIAEFKCK LELVVGSPAS
60 70 80 90 100
CMELELYGAD DKFYSKLDQE DALLGSYPVD DGCRIHVIDH SGVRLGEYED
110 120 130 140 150
VSKVEKYEIS PEAYERRQNT VRSFMKRSKL GPYNEELRAQ QEAEAAQRLS
160 170 180 190 200
EEKAQASAIS VGSRCEVRAP DHSLRRGTVM YVGLTDFKPG YWVGVRYDEP
210 220 230 240
LGKNDGSVNG KRYFECQAKY GAFVKPSAVT VGDFPEEDYG LDEM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 84 | R → S in BAB26939 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002316 mRNA Translation: BAB22009.1 AK003655 mRNA Translation: BAB22918.2 AK010438 mRNA Translation: BAB26939.1 BC010684 mRNA Translation: AAH10684.1 |
CCDSi | CCDS21083.1 |
RefSeqi | NP_079824.2, NM_025548.3 |
Genome annotation databases
Ensembli | ENSMUST00000006254; ENSMUSP00000006254; ENSMUSG00000006095 |
GeneIDi | 66411 |
KEGGi | mmu:66411 |
UCSCi | uc009gds.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002316 mRNA Translation: BAB22009.1 AK003655 mRNA Translation: BAB22918.2 AK010438 mRNA Translation: BAB26939.1 BC010684 mRNA Translation: AAH10684.1 |
CCDSi | CCDS21083.1 |
RefSeqi | NP_079824.2, NM_025548.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1V6E | NMR | - | A | 11-92 | [»] | |
1WHG | NMR | - | A | 134-233 | [»] | |
AlphaFoldDBi | Q9D1E6 | |||||
BMRBi | Q9D1E6 | |||||
SMRi | Q9D1E6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 211455, 18 interactors |
IntActi | Q9D1E6, 2 interactors |
MINTi | Q9D1E6 |
STRINGi | 10090.ENSMUSP00000006254 |
PTM databases
iPTMneti | Q9D1E6 |
PhosphoSitePlusi | Q9D1E6 |
Proteomic databases
EPDi | Q9D1E6 |
jPOSTi | Q9D1E6 |
MaxQBi | Q9D1E6 |
PaxDbi | Q9D1E6 |
PeptideAtlasi | Q9D1E6 |
PRIDEi | Q9D1E6 |
ProteomicsDBi | 263133 |
Protocols and materials databases
Antibodypediai | 29702, 291 antibodies from 29 providers |
DNASUi | 66411 |
Genome annotation databases
Ensembli | ENSMUST00000006254; ENSMUSP00000006254; ENSMUSG00000006095 |
GeneIDi | 66411 |
KEGGi | mmu:66411 |
UCSCi | uc009gds.2, mouse |
Organism-specific databases
CTDi | 1155 |
MGIi | MGI:1913661, Tbcb |
VEuPathDBi | HostDB:ENSMUSG00000006095 |
Phylogenomic databases
eggNOGi | KOG3206, Eukaryota |
GeneTreei | ENSGT00940000156119 |
HOGENOMi | CLU_067577_0_0_1 |
InParanoidi | Q9D1E6 |
OMAi | IGVKYDE |
OrthoDBi | 1550378at2759 |
PhylomeDBi | Q9D1E6 |
TreeFami | TF313444 |
Miscellaneous databases
BioGRID-ORCSi | 66411, 28 hits in 77 CRISPR screens |
ChiTaRSi | Tbcb, mouse |
EvolutionaryTracei | Q9D1E6 |
PROi | PR:Q9D1E6 |
RNActi | Q9D1E6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000006095, Expressed in facial nucleus and 305 other tissues |
Genevisiblei | Q9D1E6, MM |
Family and domain databases
CDDi | cd01789, Ubl_TBCB, 1 hit |
Gene3Di | 2.30.30.190, 1 hit |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR045172, TBCB_N_Ubl IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TBCB_MOUSE | |
Accessioni | Q9D1E6Primary (citable) accession number: Q9D1E6 Secondary accession number(s): Q9CWR6, Q9DCZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families