UniProtKB - Q9D1C8 (VPS28_MOUSE)
Vacuolar protein sorting-associated protein 28 homolog
Vps28
Functioni
GO - Molecular functioni
- protein-containing complex binding Source: GO_Central
- ubiquitin binding Source: MGI
GO - Biological processi
- negative regulation of protein ubiquitination Source: UniProtKB
- positive regulation of protein catabolic process Source: MGI
- positive regulation of ubiquitin-dependent endocytosis Source: MGI
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-917729, Endosomal Sorting Complex Required For Transport (ESCRT) |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 28 homologAlternative name(s): Caspase-activated DNase inhibitor that interacts with ASK1 Short name: CIIA ESCRT-I complex subunit VPS28 |
Gene namesi | Name:Vps28 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914164, Vps28 |
Subcellular locationi
Endosome
- Late endosome membrane By similarity; Peripheral membrane protein By similarity
Plasma membrane
- Cell membrane By similarity
Cytosol
- cytosol Source: MGI
Endosome
- early endosome Source: MGI
- endosome Source: MGI
- endosome membrane Source: MGI
- ESCRT I complex Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120952 | 1 – 221 | Vacuolar protein sorting-associated protein 28 homologAdd BLAST | 221 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9D1C8 |
MaxQBi | Q9D1C8 |
PaxDbi | Q9D1C8 |
PeptideAtlasi | Q9D1C8 |
PRIDEi | Q9D1C8 |
PTM databases
iPTMneti | Q9D1C8 |
PhosphoSitePlusi | Q9D1C8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000059323, Expressed in ankle joint and 326 other tissues |
ExpressionAtlasi | Q9D1C8, baseline and differential |
Genevisiblei | Q9D1C8, MM |
Interactioni
Subunit structurei
Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B.
Component of an ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry.
Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex.
Interacts with SNF8 and VPS25.
Interacts with CEP55 (By similarity).
By similarityBinary interactionsi
Hide detailsQ9D1C8
With | #Exp. | IntAct |
---|---|---|
Dffb [O54788] | 6 | EBI-309205,EBI-7365197 |
Map3k5 [O35099] | 3 | EBI-309205,EBI-777493 |
MAP3K5 [Q99683] from Homo sapiens. | 5 | EBI-309205,EBI-476263 |
GO - Molecular functioni
- ubiquitin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 211806, 41 interactors |
IntActi | Q9D1C8, 40 interactors |
MINTi | Q9D1C8 |
Miscellaneous databases
RNActi | Q9D1C8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 120 | VPS28 N-terminalPROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 124 – 220 | VPS28 C-terminalPROSITE-ProRule annotationAdd BLAST | 97 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00390000007486 |
HOGENOMi | CLU_076417_2_0_1 |
InParanoidi | Q9D1C8 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9D1C8 |
TreeFami | TF313364 |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFHGIPATPG VGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ
60 70 80 90 100
ALEKAYIKDC VTPNEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR
110 120 130 140 150
LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM
160 170 180 190 200
DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ
210 220
VRQMLFDLES AYNAFNRFLH A
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8VKS6 | A0A2R8VKS6_MOUSE | Vacuolar protein sorting-associated... | Vps28 | 183 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF373710 mRNA Translation: AAN71982.1 AK003699 mRNA Translation: BAB22945.1 BC013535 mRNA Translation: AAH13535.1 |
CCDSi | CCDS27579.1 |
RefSeqi | NP_001292597.1, NM_001305668.1 NP_080118.1, NM_025842.4 |
Genome annotation databases
Ensembli | ENSMUST00000078803; ENSMUSP00000077856; ENSMUSG00000115987 |
GeneIDi | 66914 |
KEGGi | mmu:66914 |
UCSCi | uc007wlc.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF373710 mRNA Translation: AAN71982.1 AK003699 mRNA Translation: BAB22945.1 BC013535 mRNA Translation: AAH13535.1 |
CCDSi | CCDS27579.1 |
RefSeqi | NP_001292597.1, NM_001305668.1 NP_080118.1, NM_025842.4 |
3D structure databases
BMRBi | Q9D1C8 |
SMRi | Q9D1C8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211806, 41 interactors |
IntActi | Q9D1C8, 40 interactors |
MINTi | Q9D1C8 |
PTM databases
iPTMneti | Q9D1C8 |
PhosphoSitePlusi | Q9D1C8 |
Proteomic databases
EPDi | Q9D1C8 |
MaxQBi | Q9D1C8 |
PaxDbi | Q9D1C8 |
PeptideAtlasi | Q9D1C8 |
PRIDEi | Q9D1C8 |
Protocols and materials databases
Antibodypediai | 14872, 188 antibodies |
DNASUi | 66914 |
Genome annotation databases
Ensembli | ENSMUST00000078803; ENSMUSP00000077856; ENSMUSG00000115987 |
GeneIDi | 66914 |
KEGGi | mmu:66914 |
UCSCi | uc007wlc.2, mouse |
Organism-specific databases
CTDi | 51160 |
MGIi | MGI:1914164, Vps28 |
Phylogenomic databases
GeneTreei | ENSGT00390000007486 |
HOGENOMi | CLU_076417_2_0_1 |
InParanoidi | Q9D1C8 |
OMAi | GPETGKW |
OrthoDBi | 1281819at2759 |
PhylomeDBi | Q9D1C8 |
TreeFami | TF313364 |
Enzyme and pathway databases
Reactomei | R-MMU-917729, Endosomal Sorting Complex Required For Transport (ESCRT) |
Miscellaneous databases
BioGRID-ORCSi | 66914, 8 hits in 12 CRISPR screens |
ChiTaRSi | Vps28, mouse |
PROi | PR:Q9D1C8 |
RNActi | Q9D1C8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000059323, Expressed in ankle joint and 326 other tissues |
ExpressionAtlasi | Q9D1C8, baseline and differential |
Genevisiblei | Q9D1C8, MM |
Family and domain databases
Gene3Di | 1.20.120.1130, 1 hit 1.20.1440.200, 1 hit |
InterProi | View protein in InterPro IPR037202, ESCRT_assembly_dom IPR007143, Vps28 IPR017899, VPS28_C IPR037206, VPS28_C_sf IPR017898, VPS28_N IPR038358, VPS28_N_sf |
PANTHERi | PTHR12937, PTHR12937, 1 hit |
Pfami | View protein in Pfam PF03997, VPS28, 1 hit |
PIRSFi | PIRSF017535, VPS28, 1 hit |
SUPFAMi | SSF140111, SSF140111, 1 hit SSF140427, SSF140427, 1 hit |
PROSITEi | View protein in PROSITE PS51310, VPS28_C, 1 hit PS51313, VPS28_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS28_MOUSE | |
Accessioni | Q9D1C8Primary (citable) accession number: Q9D1C8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families