UniProtKB - Q9D1C2 (CBY1_MOUSE)
Protein chibby homolog 1
Cby1
Functioni
Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
By similarity2 PublicationsMiscellaneous
GO - Molecular functioni
- beta-catenin binding Source: MGI
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- cardiac muscle cell differentiation Source: UniProtKB
- cilium assembly Source: MGI
- fat cell differentiation Source: UniProtKB
- floor plate development Source: MGI
- negative regulation of canonical Wnt signaling pathway Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of Wnt signaling pathway Source: MGI
- protein homotetramerization Source: MGI
- protein localization Source: MGI
Keywordsi
Biological process | Cilium biogenesis/degradation, Differentiation |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Names & Taxonomyi
Protein namesi | Recommended name: Protein chibby homolog 1Alternative name(s): Cytosolic leucine-rich protein PIGEA-14 PKD2 interactor, Golgi and endoplasmic reticulum-associated 1 |
Gene namesi | Name:Cby1 Synonyms:Cby, Pgea1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920989, Cby1 |
VEuPathDBi | HostDB:ENSMUSG00000022428 |
Subcellular locationi
Cytoskeleton
- cilium basal body By similarity
- centriole By similarity
Golgi apparatus
- Golgi apparatus By similarity
- trans-Golgi network By similarity
Nucleus
- Nucleus speckle By similarity
Cytoskeleton
- centriole Source: MGI
- ciliary basal body Source: UniProtKB
Golgi apparatus
- trans-Golgi network Source: MGI
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: MGI
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058355 | 1 – 127 | Protein chibby homolog 1Add BLAST | 127 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineBy similarity | 1 | |
Modified residuei | 20 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9D1C2 |
MaxQBi | Q9D1C2 |
PaxDbi | Q9D1C2 |
PeptideAtlasi | Q9D1C2 |
PRIDEi | Q9D1C2 |
ProteomicsDBi | 279934 |
PTM databases
iPTMneti | Q9D1C2 |
PhosphoSitePlusi | Q9D1C2 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022428, Expressed in testis and 279 other tissues |
Genevisiblei | Q9D1C2, MM |
Interactioni
Subunit structurei
Homodimer.
Interacts with polycystin-2/PKD2 and GM130.
Interacts with the C-terminal region of CTNNB1.
Interacts (C-terminus) with TCIM (C-terminus), TCIM competes with CTNNB1 for the interaction with CBY1.
Interacts with FAM92A; this interaction facilitates targeting of FAM92A to cilium basal body.
Interacts with CIBAR2.
By similarityGO - Molecular functioni
- beta-catenin binding Source: MGI
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 216224, 3 interactors |
IntActi | Q9D1C2, 1 interactor |
STRINGi | 10090.ENSMUSP00000023064 |
Miscellaneous databases
RNActi | Q9D1C2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 25 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 60 – 112 | Minimal region for the interaction with PKD2By similarityAdd BLAST | 53 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 68 – 110 | Sequence analysisAdd BLAST | 43 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S6C8, Eukaryota |
GeneTreei | ENSGT00940000153137 |
HOGENOMi | CLU_134504_0_0_1 |
InParanoidi | Q9D1C2 |
OMAi | IWMHSAR |
OrthoDBi | 1492677at2759 |
PhylomeDBi | Q9D1C2 |
TreeFami | TF324419 |
Family and domain databases
CDDi | cd07429, Cby_like, 1 hit |
InterProi | View protein in InterPro IPR028118, Chibby_fam |
Pfami | View protein in Pfam PF14645, Chibby, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPLFGSIFSP KKTPPRKSAS LSNLHSLDRS TRELELGLDY GTPTMNLAGQ
60 70 80 90 100
SLKFENGQWV ADSVISGGVD RRETQRLRKR NQQLEEENNL LRLKVDILLD
110 120
MLSETTAESH LKDKELDELK VTNRRRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF331040 mRNA Translation: AAL56061.1 AK003719 mRNA Translation: BAB22956.1 BC005733 mRNA Translation: AAH05733.1 |
CCDSi | CCDS27645.1 |
RefSeqi | NP_082910.1, NM_028634.3 |
Genome annotation databases
Ensembli | ENSMUST00000023064; ENSMUSP00000023064; ENSMUSG00000022428 |
GeneIDi | 73739 |
KEGGi | mmu:73739 |
UCSCi | uc007wuc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF331040 mRNA Translation: AAL56061.1 AK003719 mRNA Translation: BAB22956.1 BC005733 mRNA Translation: AAH05733.1 |
CCDSi | CCDS27645.1 |
RefSeqi | NP_082910.1, NM_028634.3 |
3D structure databases
SMRi | Q9D1C2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216224, 3 interactors |
IntActi | Q9D1C2, 1 interactor |
STRINGi | 10090.ENSMUSP00000023064 |
PTM databases
iPTMneti | Q9D1C2 |
PhosphoSitePlusi | Q9D1C2 |
Proteomic databases
jPOSTi | Q9D1C2 |
MaxQBi | Q9D1C2 |
PaxDbi | Q9D1C2 |
PeptideAtlasi | Q9D1C2 |
PRIDEi | Q9D1C2 |
ProteomicsDBi | 279934 |
Protocols and materials databases
Antibodypediai | 26394, 167 antibodies from 26 providers |
DNASUi | 73739 |
Genome annotation databases
Ensembli | ENSMUST00000023064; ENSMUSP00000023064; ENSMUSG00000022428 |
GeneIDi | 73739 |
KEGGi | mmu:73739 |
UCSCi | uc007wuc.1, mouse |
Organism-specific databases
CTDi | 25776 |
MGIi | MGI:1920989, Cby1 |
VEuPathDBi | HostDB:ENSMUSG00000022428 |
Phylogenomic databases
eggNOGi | ENOG502S6C8, Eukaryota |
GeneTreei | ENSGT00940000153137 |
HOGENOMi | CLU_134504_0_0_1 |
InParanoidi | Q9D1C2 |
OMAi | IWMHSAR |
OrthoDBi | 1492677at2759 |
PhylomeDBi | Q9D1C2 |
TreeFami | TF324419 |
Enzyme and pathway databases
Reactomei | R-MMU-3769402, Deactivation of the beta-catenin transactivating complex |
Miscellaneous databases
BioGRID-ORCSi | 73739, 5 hits in 65 CRISPR screens |
PROi | PR:Q9D1C2 |
RNActi | Q9D1C2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022428, Expressed in testis and 279 other tissues |
Genevisiblei | Q9D1C2, MM |
Family and domain databases
CDDi | cd07429, Cby_like, 1 hit |
InterProi | View protein in InterPro IPR028118, Chibby_fam |
Pfami | View protein in Pfam PF14645, Chibby, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CBY1_MOUSE | |
Accessioni | Q9D1C2Primary (citable) accession number: Q9D1C2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families