UniProtKB - Q9D0T1 (NH2L1_MOUSE)
NHP2-like protein 1
Snu13
Functioni
Involved in pre-mRNA splicing as component of the spliceosome. Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.
By similarityGO - Molecular functioni
- ATPase binding Source: MGI
- box C/D RNA binding Source: CAFA
- RNA binding Source: GO_Central
- U3 snoRNA binding Source: MGI
- U4atac snRNA binding Source: UniProtKB
- U4 snRNA binding Source: MGI
GO - Biological processi
- box C/D snoRNP assembly Source: CAFA
- maturation of LSU-rRNA Source: GO_Central
- maturation of SSU-rRNA Source: GO_Central
- mRNA splicing, via spliceosome Source: UniProtKB
- single fertilization Source: MGI
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: NHP2-like protein 1Alternative name(s): Fertilization antigen 11 Publication Short name: FA-1 High mobility group-like nuclear protein 2 homolog 1 Sperm-specific antigen 1 U4/U6.U5 small nuclear ribonucleoprotein SNU13By similarity U4/U6.U5 tri-snRNP 15.5 kDa protein Cleaved into the following chain: |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:893586, Snu13 |
VEuPathDBi | HostDB:ENSMUSG00000063480 |
Subcellular locationi
Nucleus
- dense fibrillar component Source: MGI
- nucleolus Source: MGI
- nucleus Source: UniProtKB
- precatalytic spliceosome Source: GO_Central
- U2-type precatalytic spliceosome Source: UniProtKB
- U4/U6 x U5 tri-snRNP complex Source: UniProtKB
- U4atac snRNP Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- box C/D RNP complex Source: CAFA
- protein-containing complex Source: MGI
- small-subunit processome Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423262 | 1 – 128 | NHP2-like protein 1Add BLAST | 128 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000136779 | 2 – 128 | NHP2-like protein 1, N-terminally processedAdd BLAST | 127 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylthreonine; in NHP2-like protein 1, N-terminally processedBy similarity | 1 | |
Modified residuei | 21 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 122 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9D0T1 |
jPOSTi | Q9D0T1 |
MaxQBi | Q9D0T1 |
PaxDbi | Q9D0T1 |
PeptideAtlasi | Q9D0T1 |
PRIDEi | Q9D0T1 |
ProteomicsDBi | 287506 |
TopDownProteomicsi | Q9D0T1 |
PTM databases
iPTMneti | Q9D0T1 |
PhosphoSitePlusi | Q9D0T1 |
SwissPalmi | Q9D0T1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000063480, Expressed in epiblast (generic) and 72 other tissues |
Genevisiblei | Q9D0T1, MM |
Interactioni
Subunit structurei
Identified in the spliceosome B complex.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39.
Interacts with RAD17 and PRPF31. The complex formed by SNU13 and PRPF31 binds U4 snRNA. The complex formed by SNU13 and PRPF31 binds also U4atac snRNA, a characteristic component of specific, less abundant spliceosomal complexes.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 61 | Interaction with U4 snRNA and U4atac snRNABy similarity | 1 | |
Sitei | 86 | Interaction with U4 snRNA and U4atac snRNABy similarity | 1 |
GO - Molecular functioni
- ATPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 203509, 50 interactors |
IntActi | Q9D0T1, 52 interactors |
MINTi | Q9D0T1 |
STRINGi | 10090.ENSMUSP00000091840 |
Miscellaneous databases
RNActi | Q9D0T1, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 36 – 48 | Interaction with U4 snRNA and U4atac snRNABy similarityAdd BLAST | 13 | |
Regioni | 96 – 128 | Important for U4 snRNA-bindingBy similarityAdd BLAST | 33 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3387, Eukaryota |
GeneTreei | ENSGT00550000074840 |
HOGENOMi | CLU_084513_4_1_1 |
InParanoidi | Q9D0T1 |
OMAi | KPFYVRF |
OrthoDBi | 1497609at2759 |
PhylomeDBi | Q9D0T1 |
TreeFami | TF300184 |
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit |
InterProi | View protein in InterPro IPR002415, H/ACA_rnp_Nhp2_euk IPR029064, L30e-like IPR004038, Ribosomal_L7Ae/L30e/S12e/Gad45 IPR018492, Ribosomal_L7Ae/L8/Nhp2 IPR004037, Ribosomal_L7Ae_CS |
Pfami | View protein in Pfam PF01248, Ribosomal_L7Ae, 1 hit |
PRINTSi | PR00881, L7ARS6FAMILY PR00883, NUCLEARHMG |
SUPFAMi | SSF55315, SSF55315, 1 hit |
PROSITEi | View protein in PROSITE PS01082, RIBOSOMAL_L7AE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI
60 70 80 90 100
SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQALG RACGVSRPVI
110 120
ACSVTIKEGS QLKQQIQSIQ QSIERLLV
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | K → R in BAB23329 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95114 mRNA Translation: AAC53177.1 Frameshift. AK004489 mRNA Translation: BAB23329.1 AK134943 mRNA Translation: BAE22349.1 AK145608 mRNA Translation: BAE26537.1 AK168930 mRNA Translation: BAE40743.1 AK169103 mRNA Translation: BAE40885.1 BC026755 mRNA Translation: AAH26755.1 BC054450 mRNA Translation: AAH54450.1 BC083315 mRNA Translation: AAH83315.1 |
CCDSi | CCDS37154.1 |
PIRi | T10105 |
RefSeqi | NP_035612.2, NM_011482.4 XP_003689233.1, XM_003689185.5 |
Genome annotation databases
Ensembli | ENSMUST00000080622; ENSMUSP00000091840; ENSMUSG00000063480 |
GeneIDi | 20826 |
KEGGi | mmu:20826 |
UCSCi | uc007wya.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95114 mRNA Translation: AAC53177.1 Frameshift. AK004489 mRNA Translation: BAB23329.1 AK134943 mRNA Translation: BAE22349.1 AK145608 mRNA Translation: BAE26537.1 AK168930 mRNA Translation: BAE40743.1 AK169103 mRNA Translation: BAE40885.1 BC026755 mRNA Translation: AAH26755.1 BC054450 mRNA Translation: AAH54450.1 BC083315 mRNA Translation: AAH83315.1 |
CCDSi | CCDS37154.1 |
PIRi | T10105 |
RefSeqi | NP_035612.2, NM_011482.4 XP_003689233.1, XM_003689185.5 |
3D structure databases
BMRBi | Q9D0T1 |
SMRi | Q9D0T1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203509, 50 interactors |
IntActi | Q9D0T1, 52 interactors |
MINTi | Q9D0T1 |
STRINGi | 10090.ENSMUSP00000091840 |
PTM databases
iPTMneti | Q9D0T1 |
PhosphoSitePlusi | Q9D0T1 |
SwissPalmi | Q9D0T1 |
Proteomic databases
EPDi | Q9D0T1 |
jPOSTi | Q9D0T1 |
MaxQBi | Q9D0T1 |
PaxDbi | Q9D0T1 |
PeptideAtlasi | Q9D0T1 |
PRIDEi | Q9D0T1 |
ProteomicsDBi | 287506 |
TopDownProteomicsi | Q9D0T1 |
Protocols and materials databases
Antibodypediai | 27035, 106 antibodies from 26 providers |
DNASUi | 20826 |
Genome annotation databases
Ensembli | ENSMUST00000080622; ENSMUSP00000091840; ENSMUSG00000063480 |
GeneIDi | 20826 |
KEGGi | mmu:20826 |
UCSCi | uc007wya.1, mouse |
Organism-specific databases
CTDi | 4809 |
MGIi | MGI:893586, Snu13 |
VEuPathDBi | HostDB:ENSMUSG00000063480 |
Phylogenomic databases
eggNOGi | KOG3387, Eukaryota |
GeneTreei | ENSGT00550000074840 |
HOGENOMi | CLU_084513_4_1_1 |
InParanoidi | Q9D0T1 |
OMAi | KPFYVRF |
OrthoDBi | 1497609at2759 |
PhylomeDBi | Q9D0T1 |
TreeFami | TF300184 |
Enzyme and pathway databases
Reactomei | R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72165, mRNA Splicing - Minor Pathway |
Miscellaneous databases
BioGRID-ORCSi | 20826, 23 hits in 43 CRISPR screens |
ChiTaRSi | Nhp2l1, mouse |
PROi | PR:Q9D0T1 |
RNActi | Q9D0T1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000063480, Expressed in epiblast (generic) and 72 other tissues |
Genevisiblei | Q9D0T1, MM |
Family and domain databases
Gene3Di | 3.30.1330.30, 1 hit |
InterProi | View protein in InterPro IPR002415, H/ACA_rnp_Nhp2_euk IPR029064, L30e-like IPR004038, Ribosomal_L7Ae/L30e/S12e/Gad45 IPR018492, Ribosomal_L7Ae/L8/Nhp2 IPR004037, Ribosomal_L7Ae_CS |
Pfami | View protein in Pfam PF01248, Ribosomal_L7Ae, 1 hit |
PRINTSi | PR00881, L7ARS6FAMILY PR00883, NUCLEARHMG |
SUPFAMi | SSF55315, SSF55315, 1 hit |
PROSITEi | View protein in PROSITE PS01082, RIBOSOMAL_L7AE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NH2L1_MOUSE | |
Accessioni | Q9D0T1Primary (citable) accession number: Q9D0T1 Secondary accession number(s): O08754, Q3UY64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 161 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families