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UniProtKB - Q9D0S9 (HINT2_MOUSE)
Protein
Adenosine 5'-monophosphoramidase HINT2
Gene
Hint2
Organism
Mus musculus (Mouse)
Status
Functioni
Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (By similarity).
Hydrolyzes adenosine 5'-O-p-nitrophenylphosphoramidate (AMP-pNA) (By similarity).
May be involved in steroid biosynthesis (By similarity).
May play a role in apoptosis (By similarity).
By similarityCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 63 | AMPBy similarity | 1 | |
Binding sitei | 80 | AMPBy similarity | 1 | |
Binding sitei | 136 | AMPBy similarity | 1 | |
Active sitei | 149 | Tele-AMP-histidine intermediateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 142 – 145 | AMPBy similarity | 4 | |
Nucleotide bindingi | 149 – 151 | AMPBy similarity | 3 |
GO - Molecular functioni
- adenosine 5'-monophosphoramidase activity Source: UniProtKB
- hydrolase activity Source: GO_Central
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- lipid catabolic process Source: MGI
- negative regulation of peptidyl-lysine acetylation Source: MGI
- steroid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Apoptosis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-9013405, RHOD GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosine 5'-monophosphoramidase HINT2By similarity (EC:3.9.1.-By similarity)Alternative name(s): HINT-3By similarity Histidine triad nucleotide-binding protein 2, mitochondrialCurated Short name: HINT-2By similarity |
Gene namesi | Name:Hint2Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1916167, Hint2 |
VEuPathDBi | HostDB:ENSMUSG00000028470 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 17 | MitochondrionSequence analysisAdd BLAST | 17 | |
ChainiPRO_0000109787 | 18 – 163 | Adenosine 5'-monophosphoramidase HINT2Add BLAST | 146 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 45 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 119 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 128 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 128 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 139 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9D0S9 |
jPOSTi | Q9D0S9 |
MaxQBi | Q9D0S9 |
PaxDbi | Q9D0S9 |
PRIDEi | Q9D0S9 |
ProteomicsDBi | 273111 |
PTM databases
iPTMneti | Q9D0S9 |
PhosphoSitePlusi | Q9D0S9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028470, Expressed in adult mammalian kidney and 304 other tissues |
ExpressionAtlasi | Q9D0S9, baseline and differential |
Genevisiblei | Q9D0S9, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 213113, 3 interactors |
IntActi | Q9D0S9, 1 interactor |
STRINGi | 10090.ENSMUSP00000030192 |
Miscellaneous databases
RNActi | Q9D0S9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 163 | HITPROSITE-ProRule annotationAdd BLAST | 109 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 147 – 151 | Histidine triad motif | 5 |
Sequence similaritiesi
Belongs to the HINT family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3275, Eukaryota |
GeneTreei | ENSGT00940000157905 |
HOGENOMi | CLU_056776_8_0_1 |
InParanoidi | Q9D0S9 |
OMAi | SDCLFCK |
OrthoDBi | 1190598at2759 |
PhylomeDBi | Q9D0S9 |
TreeFami | TF314862 |
Family and domain databases
Gene3Di | 3.30.428.10, 1 hit |
InterProi | View protein in InterPro IPR019808, Histidine_triad_CS IPR001310, Histidine_triad_HIT IPR011146, HIT-like IPR036265, HIT-like_sf |
PANTHERi | PTHR23089, PTHR23089, 1 hit |
Pfami | View protein in Pfam PF01230, HIT, 1 hit |
PRINTSi | PR00332, HISTRIAD |
SUPFAMi | SSF54197, SSF54197, 1 hit |
PROSITEi | View protein in PROSITE PS00892, HIT_1, 1 hit PS51084, HIT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9D0S9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAAVLLAVG LRAARRTLAA AGARGAQVRG NAGVSDGSEV AKAQKAAPGG
60 70 80 90 100
ASPTIFSRIL DRSLPADILY EDQQCLVFRD VAPQAPVHFL VIPRKPIPRI
110 120 130 140 150
SQAEEDDQQL LGHLLLVAKK IAQAQGLKDG YRLVVNDGKM GAQSVYHLHI
160
HVLGGRQLQW PPG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF356874 mRNA Translation: AAM00220.1 AY040765 Genomic DNA Translation: AAK94774.1 AK004497 mRNA Translation: BAB23334.1 |
CCDSi | CCDS18106.1 |
RefSeqi | NP_081147.1, NM_026871.1 |
Genome annotation databases
Ensembli | ENSMUST00000030192; ENSMUSP00000030192; ENSMUSG00000028470 |
GeneIDi | 68917 |
KEGGi | mmu:68917 |
UCSCi | uc008sqs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF356874 mRNA Translation: AAM00220.1 AY040765 Genomic DNA Translation: AAK94774.1 AK004497 mRNA Translation: BAB23334.1 |
CCDSi | CCDS18106.1 |
RefSeqi | NP_081147.1, NM_026871.1 |
3D structure databases
AlphaFoldDBi | Q9D0S9 |
SMRi | Q9D0S9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 213113, 3 interactors |
IntActi | Q9D0S9, 1 interactor |
STRINGi | 10090.ENSMUSP00000030192 |
PTM databases
iPTMneti | Q9D0S9 |
PhosphoSitePlusi | Q9D0S9 |
Proteomic databases
EPDi | Q9D0S9 |
jPOSTi | Q9D0S9 |
MaxQBi | Q9D0S9 |
PaxDbi | Q9D0S9 |
PRIDEi | Q9D0S9 |
ProteomicsDBi | 273111 |
Protocols and materials databases
Antibodypediai | 11787, 136 antibodies from 18 providers |
DNASUi | 68917 |
Genome annotation databases
Ensembli | ENSMUST00000030192; ENSMUSP00000030192; ENSMUSG00000028470 |
GeneIDi | 68917 |
KEGGi | mmu:68917 |
UCSCi | uc008sqs.1, mouse |
Organism-specific databases
CTDi | 84681 |
MGIi | MGI:1916167, Hint2 |
VEuPathDBi | HostDB:ENSMUSG00000028470 |
Phylogenomic databases
eggNOGi | KOG3275, Eukaryota |
GeneTreei | ENSGT00940000157905 |
HOGENOMi | CLU_056776_8_0_1 |
InParanoidi | Q9D0S9 |
OMAi | SDCLFCK |
OrthoDBi | 1190598at2759 |
PhylomeDBi | Q9D0S9 |
TreeFami | TF314862 |
Enzyme and pathway databases
Reactomei | R-MMU-9013405, RHOD GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 68917, 4 hits in 73 CRISPR screens |
ChiTaRSi | Hint2, mouse |
PROi | PR:Q9D0S9 |
RNActi | Q9D0S9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028470, Expressed in adult mammalian kidney and 304 other tissues |
ExpressionAtlasi | Q9D0S9, baseline and differential |
Genevisiblei | Q9D0S9, MM |
Family and domain databases
Gene3Di | 3.30.428.10, 1 hit |
InterProi | View protein in InterPro IPR019808, Histidine_triad_CS IPR001310, Histidine_triad_HIT IPR011146, HIT-like IPR036265, HIT-like_sf |
PANTHERi | PTHR23089, PTHR23089, 1 hit |
Pfami | View protein in Pfam PF01230, HIT, 1 hit |
PRINTSi | PR00332, HISTRIAD |
SUPFAMi | SSF54197, SSF54197, 1 hit |
PROSITEi | View protein in PROSITE PS00892, HIT_1, 1 hit PS51084, HIT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HINT2_MOUSE | |
Accessioni | Q9D0S9Primary (citable) accession number: Q9D0S9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families