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Protein

Protein ERGIC-53

Gene

Lman1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96CarbohydratePROSITE-ProRule annotation1
Binding sitei129CarbohydratePROSITE-ProRule annotation1
Metal bindingi160CalciumPROSITE-ProRule annotation1
Metal bindingi162Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi164CalciumPROSITE-ProRule annotation1
Binding sitei164CarbohydratePROSITE-ProRule annotation1
Binding sitei186CarbohydratePROSITE-ProRule annotation1
Metal bindingi189CalciumPROSITE-ProRule annotation1
Sitei508Required for ER exportBy similarity1

GO - Molecular functioni

  • identical protein binding Source: MGI
  • mannose binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandLectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-948021 Transport to the Golgi and subsequent modification

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ERGIC-53
Alternative name(s):
ER-Golgi intermediate compartment 53 kDa protein
Lectin mannose-binding 1
p58
Gene namesi
Name:Lman1
Synonyms:Ergic53
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1917611 Lman1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 484LumenalSequence analysisAdd BLAST454
Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
Topological domaini506 – 517CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000001766131 – 517Protein ERGIC-53Add BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi198 ↔ 238PROSITE-ProRule annotation
Modified residuei433PhosphoserineBy similarity1
Disulfide bondi473InterchainPROSITE-ProRule annotation
Disulfide bondi482InterchainPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9D0F3
MaxQBiQ9D0F3
PaxDbiQ9D0F3
PeptideAtlasiQ9D0F3
PRIDEiQ9D0F3

PTM databases

iPTMnetiQ9D0F3
PhosphoSitePlusiQ9D0F3
SwissPalmiQ9D0F3

Expressioni

Gene expression databases

BgeeiENSMUSG00000041891 Expressed in 295 organ(s), highest expression level in islet of Langerhans
CleanExiMM_LMAN1
ExpressionAtlasiQ9D0F3 baseline and differential
GenevisibleiQ9D0F3 MM

Interactioni

Subunit structurei

Exists both as a covalent disulfide-linked homohexamer, and a complex of three disulfide-linked dimers non-covalently kept together. Interacts with MCFD2. May interact with TMEM115. Interacts with RAB3GAP1 and RAB3GAP2. Interacts with UBXN6.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214001, 2 interactors
IntActiQ9D0F3, 6 interactors
MINTiQ9D0F3
STRINGi10090.ENSMUSP00000040140

Structurei

3D structure databases

ProteinModelPortaliQ9D0F3
SMRiQ9D0F3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 275L-type lectin-likePROSITE-ProRule annotationAdd BLAST224

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 261Carbohydrate bindingPROSITE-ProRule annotation3
Regioni506 – 517Mediates interaction with RAB3GAP1, RAB3GAP2 and UBXN6By similarityAdd BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi516 – 517ER export motifBy similarity2

Domaini

The FF ER export motif at the C-terminus is not sufficient to support endoplasmic reticulum exit, and needs assistance of Gln-508 for proper recognition of COPII coat components.By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3838 Eukaryota
ENOG410ZFRX LUCA
GeneTreeiENSGT00530000062977
HOVERGENiHBG052332
InParanoidiQ9D0F3
KOiK10080
OMAiNMIIPAQ
OrthoDBiEOG091G08WZ
PhylomeDBiQ9D0F3
TreeFamiTF313311

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR005052 Lectin_leg
IPR033085 LMAN1
PANTHERiPTHR12223:SF32 PTHR12223:SF32, 1 hit
PfamiView protein in Pfam
PF03388 Lectin_leg-like, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51328 L_LECTIN_LIKE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9D0F3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVSRRRVPQ AGARSFFCAL LLSFSQFTGS DGTGGDAAAP GAAGTQAELP
60 70 80 90 100
HRRFEYKYSF KGPHLVQSDG TVPFWAHAGN AIPSADQIRI APSLKSQRGS
110 120 130 140 150
VWTKAKAAFE NWEVEVTFRV TGRGRIGADG LAIWYTENQG LDGPVFGSAD
160 170 180 190 200
TWNGVGIFFD SFDNDGKKNN PAIVVIGNNG QINYDHQNDG ATQALASCQR
210 220 230 240 250
DFRNKPYPVR AKITYYQKTL TVMINNGFTP DKNDYEFCAK VENMVIPTQG
260 270 280 290 300
HFGISAATGG LADDHDVLSF LTFQLTEPGK EPPTAEKDIS EKEKEKYQEE
310 320 330 340 350
FEHFQQELDK KKEEFQKGHP DLQGQPADDI FESIGDRELR QVFEGQNRIH
360 370 380 390 400
LEIKQLNRQL DMILDEQRRY VSSLTEEISR RGAGTPGQPG QVSQQELDTV
410 420 430 440 450
VKSQQEILRQ VNEVKNSMSE TVRLVSGIQH PGSAGVYETT QHFMDIKEHL
460 470 480 490 500
HVVKRDIDSL AQRSMPSNEK PKCPDLPPFP SCLSTIHFVI FVVVQTVLFV
510
GYIMYRTQQE AAAKKFF
Length:517
Mass (Da):57,789
Last modified:June 1, 2001 - v1
Checksum:iBA9FCFDEBCC5656D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z5B9D3Z5B9_MOUSE
Protein ERGIC-53
Lman1
289Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011495 mRNA Translation: BAB27655.1
BC057165 mRNA Translation: AAH57165.1
CCDSiCCDS29313.1
RefSeqiNP_001165533.1, NM_001172062.1
NP_081676.1, NM_027400.3
UniGeneiMm.290857
Mm.449042

Genome annotation databases

EnsembliENSMUST00000048260; ENSMUSP00000040140; ENSMUSG00000041891
ENSMUST00000120461; ENSMUSP00000113326; ENSMUSG00000041891
GeneIDi70361
KEGGimmu:70361
UCSCiuc008ffn.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011495 mRNA Translation: BAB27655.1
BC057165 mRNA Translation: AAH57165.1
CCDSiCCDS29313.1
RefSeqiNP_001165533.1, NM_001172062.1
NP_081676.1, NM_027400.3
UniGeneiMm.290857
Mm.449042

3D structure databases

ProteinModelPortaliQ9D0F3
SMRiQ9D0F3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214001, 2 interactors
IntActiQ9D0F3, 6 interactors
MINTiQ9D0F3
STRINGi10090.ENSMUSP00000040140

PTM databases

iPTMnetiQ9D0F3
PhosphoSitePlusiQ9D0F3
SwissPalmiQ9D0F3

Proteomic databases

EPDiQ9D0F3
MaxQBiQ9D0F3
PaxDbiQ9D0F3
PeptideAtlasiQ9D0F3
PRIDEiQ9D0F3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048260; ENSMUSP00000040140; ENSMUSG00000041891
ENSMUST00000120461; ENSMUSP00000113326; ENSMUSG00000041891
GeneIDi70361
KEGGimmu:70361
UCSCiuc008ffn.2 mouse

Organism-specific databases

CTDi3998
MGIiMGI:1917611 Lman1

Phylogenomic databases

eggNOGiKOG3838 Eukaryota
ENOG410ZFRX LUCA
GeneTreeiENSGT00530000062977
HOVERGENiHBG052332
InParanoidiQ9D0F3
KOiK10080
OMAiNMIIPAQ
OrthoDBiEOG091G08WZ
PhylomeDBiQ9D0F3
TreeFamiTF313311

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-948021 Transport to the Golgi and subsequent modification

Miscellaneous databases

ChiTaRSiLman1 mouse
PROiPR:Q9D0F3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041891 Expressed in 295 organ(s), highest expression level in islet of Langerhans
CleanExiMM_LMAN1
ExpressionAtlasiQ9D0F3 baseline and differential
GenevisibleiQ9D0F3 MM

Family and domain databases

InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR005052 Lectin_leg
IPR033085 LMAN1
PANTHERiPTHR12223:SF32 PTHR12223:SF32, 1 hit
PfamiView protein in Pfam
PF03388 Lectin_leg-like, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51328 L_LECTIN_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLMAN1_MOUSE
AccessioniPrimary (citable) accession number: Q9D0F3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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