UniProtKB - Q9D099 (ACER3_MOUSE)
Alkaline ceramidase 3
Acer3
Functioni
Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:26474409).
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:26474409).
Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma (By similarity).
Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function (PubMed:26474409).
By regulating the levels of pro-inflammatory ceramides in immune cells and tissues, may modulate the inflammatory response (PubMed:26938296).
1 PublicationBy similarity2 PublicationsCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sphing-4-enine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + sphing-4-enineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sphinganine = (5Z,8Z,11Z,14Z)-eicosatetraenoate + sphinganineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- H2O + N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-(4R)-hydroxysphinganine = (4R)-hydroxysphinganine + (5Z,8Z,11Z,14Z)-eicosatetraenoateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- H2O + N-(11Z-eicosenoyl)-(4R)-hydroxysphinganine = (11Z)-eicosenoate + (4R)-hydroxysphinganineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- H2O + N-(9Z-octadecenoyl)-(4R)-hydroxysphinganine = (4R)-hydroxysphinganine + (9Z)-octadecenoateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.5.1.231 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
: sphingolipid metabolism Pathwayi
This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 19 | CalciumBy similarity | 1 | |
Metal bindingi | 20 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 22 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 24 | CalciumBy similarity | 1 | |
Metal bindingi | 33 | CalciumBy similarity | 1 | |
Metal bindingi | 81 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 217 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 221 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- ceramidase activity Source: UniProtKB-EC
- dihydroceramidase activity Source: UniProtKB
- N-acylsphingosine amidohydrolase activity Source: UniProtKB
- phytoceramidase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- ceramide catabolic process Source: UniProtKB
- inflammatory response Source: UniProtKB
- myelination Source: UniProtKB
- phytosphingosine biosynthetic process Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- regulation of programmed cell death Source: UniProtKB
- sphingosine biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.5.1.23, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
UniPathwayi | UPA00222 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Acer3 Synonyms:Aphc, Phca |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913440, Acer3 |
VEuPathDBi | HostDB:ENSMUSG00000030760 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Golgi apparatus
- integral component of Golgi membrane Source: UniProtKB
Other locations
- integral component of membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicCuratedAdd BLAST | 33 | |
Transmembranei | 34 – 55 | HelicalBy similarityAdd BLAST | 22 | |
Topological domaini | 56 – 61 | LumenalCurated | 6 | |
Transmembranei | 62 – 82 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 83 – 87 | CytoplasmicCurated | 5 | |
Transmembranei | 88 – 108 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 109 – 118 | LumenalCurated | 10 | |
Transmembranei | 119 – 139 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 140 – 141 | CytoplasmicCurated | 2 | |
Transmembranei | 142 – 162 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 163 – 173 | LumenalCuratedAdd BLAST | 11 | |
Transmembranei | 174 – 194 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 195 – 215 | CytoplasmicCuratedAdd BLAST | 21 | |
Transmembranei | 216 – 236 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 237 – 267 | LumenalCuratedAdd BLAST | 31 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212464 | 1 – 267 | Alkaline ceramidase 3Add BLAST | 267 |
Proteomic databases
MaxQBi | Q9D099 |
PaxDbi | Q9D099 |
PRIDEi | Q9D099 |
ProteomicsDBi | 285837 |
PTM databases
PhosphoSitePlusi | Q9D099 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000030760, Expressed in conjunctival fornix and 260 other tissues |
Genevisiblei | Q9D099, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033020 |
Miscellaneous databases
RNActi | Q9D099, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2329, Eukaryota |
GeneTreei | ENSGT00730000110920 |
HOGENOMi | CLU_063293_3_2_1 |
InParanoidi | Q9D099 |
OMAi | IMFEPLR |
OrthoDBi | 969354at2759 |
PhylomeDBi | Q9D099 |
TreeFami | TF313019 |
Family and domain databases
InterProi | View protein in InterPro IPR008901, ACER |
PANTHERi | PTHR46187, PTHR46187, 1 hit |
Pfami | View protein in Pfam PF05875, Ceramidase, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAPAVDRKGY WGPTTSTLDW CEENYVVTLF VAEFWNTVSN LIMIIPPIFG
60 70 80 90 100
AIQGIRDRLE KRYIAAYLAL TVVGMGSWCF HMTLKYEMQL LDELPMIYSC
110 120 130 140 150
CIFVYCMFEC FKTKSSINYH LLFTLFLYSL TVTTIYLKVK EPIFHQVMYG
160 170 180 190 200
MLVFTLVLRS IYIVTWVYPW LRGLGYTSLT VFLLGFLLWN IDNIFCDSLR
210 220 230 240 250
NFRKRVPPVL GVTTQFHAWW HILTGLGSYL HILFSLYTRT LYLRYRPKVK
260
FLFGIWPAVM FEPQRKH
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YTU8 | D3YTU8_MOUSE | Alkaline ceramidase | Acer3 | 225 | Annotation score: | ||
D6RJ31 | D6RJ31_MOUSE | Alkaline ceramidase | Acer3 | 90 | Annotation score: | ||
F6RA04 | F6RA04_MOUSE | Alkaline ceramidase | Acer3 | 174 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 110 | C → F in BAB23250 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 262 | E → D in BAB30708 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004287 mRNA Translation: BAB23250.1 AK011668 mRNA Translation: BAB27768.1 AK017361 mRNA Translation: BAB30708.1 AK080977 mRNA Translation: BAC38101.1 AK150629 mRNA Translation: BAE29719.1 AK162403 mRNA Translation: BAE36897.1 |
CCDSi | CCDS21468.1 |
RefSeqi | NP_079684.2, NM_025408.2 |
Genome annotation databases
Ensembli | ENSMUST00000033020; ENSMUSP00000033020; ENSMUSG00000030760 |
GeneIDi | 66190 |
KEGGi | mmu:66190 |
UCSCi | uc009ikg.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004287 mRNA Translation: BAB23250.1 AK011668 mRNA Translation: BAB27768.1 AK017361 mRNA Translation: BAB30708.1 AK080977 mRNA Translation: BAC38101.1 AK150629 mRNA Translation: BAE29719.1 AK162403 mRNA Translation: BAE36897.1 |
CCDSi | CCDS21468.1 |
RefSeqi | NP_079684.2, NM_025408.2 |
3D structure databases
AlphaFoldDBi | Q9D099 |
SMRi | Q9D099 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033020 |
PTM databases
PhosphoSitePlusi | Q9D099 |
Proteomic databases
MaxQBi | Q9D099 |
PaxDbi | Q9D099 |
PRIDEi | Q9D099 |
ProteomicsDBi | 285837 |
Protocols and materials databases
Antibodypediai | 53464, 126 antibodies from 25 providers |
DNASUi | 66190 |
Genome annotation databases
Ensembli | ENSMUST00000033020; ENSMUSP00000033020; ENSMUSG00000030760 |
GeneIDi | 66190 |
KEGGi | mmu:66190 |
UCSCi | uc009ikg.1, mouse |
Organism-specific databases
CTDi | 55331 |
MGIi | MGI:1913440, Acer3 |
VEuPathDBi | HostDB:ENSMUSG00000030760 |
Phylogenomic databases
eggNOGi | KOG2329, Eukaryota |
GeneTreei | ENSGT00730000110920 |
HOGENOMi | CLU_063293_3_2_1 |
InParanoidi | Q9D099 |
OMAi | IMFEPLR |
OrthoDBi | 969354at2759 |
PhylomeDBi | Q9D099 |
TreeFami | TF313019 |
Enzyme and pathway databases
UniPathwayi | UPA00222 |
BRENDAi | 3.5.1.23, 3474 |
Reactomei | R-MMU-1660661, Sphingolipid de novo biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 66190, 2 hits in 74 CRISPR screens |
ChiTaRSi | Acer3, mouse |
PROi | PR:Q9D099 |
RNActi | Q9D099, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030760, Expressed in conjunctival fornix and 260 other tissues |
Genevisiblei | Q9D099, MM |
Family and domain databases
InterProi | View protein in InterPro IPR008901, ACER |
PANTHERi | PTHR46187, PTHR46187, 1 hit |
Pfami | View protein in Pfam PF05875, Ceramidase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACER3_MOUSE | |
Accessioni | Q9D099Primary (citable) accession number: Q9D099 Secondary accession number(s): Q542R2, Q9D0X4, Q9D3J4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 14, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families