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Protein

Kinetochore protein Spc24

Gene

Spc24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Spc24
Gene namesi
Name:Spc24
Synonyms:Spbc24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1914879 Spc24

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495601 – 201Kinetochore protein Spc24Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9D083
PaxDbiQ9D083
PeptideAtlasiQ9D083
PRIDEiQ9D083

PTM databases

iPTMnetiQ9D083
PhosphoSitePlusiQ9D083

Expressioni

Gene expression databases

BgeeiENSMUSG00000074476 Expressed in 172 organ(s), highest expression level in pes
ExpressionAtlasiQ9D083 baseline and differential
GenevisibleiQ9D083 MM

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end (By similarity).By similarity

Protein-protein interaction databases

ComplexPortaliCPX-551 Ndc80 complex
IntActiQ9D083, 1 interactor
MINTiQ9D083
STRINGi10090.ENSMUSP00000096541

Structurei

3D structure databases

ProteinModelPortaliQ9D083
SMRiQ9D083
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Interaction with the N-terminus of SPBC25By similarityAdd BLAST131
Regioni1 – 69Interaction with the NDC80-CDCA1 subcomplexBy similarityAdd BLAST69
Regioni132 – 197Interaction with the C-terminus of SPBC25By similarityAdd BLAST66

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili21 – 132Sequence analysisAdd BLAST112

Sequence similaritiesi

Belongs to the SPC24 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IYQI Eukaryota
ENOG4111TPD LUCA
GeneTreeiENSGT00390000005584
HOGENOMiHOG000004855
HOVERGENiHBG083027
InParanoidiQ9D083
KOiK11549
OMAiGMVKGIH
OrthoDBiEOG091G0N4A
PhylomeDBiQ9D083
TreeFamiTF333217

Family and domain databases

InterProiView protein in InterPro
IPR013252 Ndc80_Spc24
PANTHERiPTHR22142 PTHR22142, 1 hit
PfamiView protein in Pfam
PF08286 Spc24, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9D083-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAFRDMVEV SNWLLSLLGA NRAEAQQRRL LGSYEQMMER LLEMQDGAYR
60 70 80 90 100
QLRETLAVEE EVAQSLLELK ECTRQGDTEL QQLEVELQRT SKEDTCVQAR
110 120 130 140 150
LRQLITELQE LREMEEELQR QERDVDEDNT VTIPSAVYVA HLYHQISKIQ
160 170 180 190 200
WDYECEPGMI KGIHHGPTVA QPIHLDSAQL SPKFISDYLW SLVDTTWEPE

P
Length:201
Mass (Da):23,416
Last modified:June 1, 2001 - v1
Checksum:i76192EE5B7114B57
GO
Isoform 2 (identifier: Q9D083-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-160: YVAHLYHQISKIQWDYECEPGMI → PPRPHSGPARPLGQCTALAEVHQ
     161-201: Missing.

Show »
Length:160
Mass (Da):18,418
Checksum:iC8917902F8D64455
GO
Isoform 3 (identifier: Q9D083-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-201: Missing.

Note: No experimental confirmation available.
Show »
Length:137
Mass (Da):16,016
Checksum:i8F06282CED48A1B7
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SV84A0A1L1SV84_MOUSE
Kinetochore protein Spc24
Spc24
160Annotation score:
A0A1L1SSK2A0A1L1SSK2_MOUSE
Kinetochore protein Spc24
Spc24
42Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020515138 – 201Missing in isoform 3. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_020516138 – 160YVAHL…EPGMI → PPRPHSGPARPLGQCTALAE VHQ in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_020517161 – 201Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011728 mRNA Translation: BAB27804.1
AK031680 mRNA Translation: BAC27510.1
AK075950 mRNA Translation: BAC36077.1
AK153185 mRNA Translation: BAE31786.1
AK167877 mRNA Translation: BAE39891.1
BC086683 mRNA Translation: AAH86683.1
BC116238 mRNA Translation: AAI16239.1
CCDSiCCDS40554.1 [Q9D083-1]
RefSeqiNP_080558.1, NM_026282.5 [Q9D083-1]
UniGeneiMm.295642

Genome annotation databases

EnsembliENSMUST00000098942; ENSMUSP00000096541; ENSMUSG00000074476 [Q9D083-1]
ENSMUST00000217382; ENSMUSP00000150475; ENSMUSG00000074476 [Q9D083-3]
GeneIDi67629
KEGGimmu:67629
UCSCiuc009oml.1 mouse [Q9D083-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011728 mRNA Translation: BAB27804.1
AK031680 mRNA Translation: BAC27510.1
AK075950 mRNA Translation: BAC36077.1
AK153185 mRNA Translation: BAE31786.1
AK167877 mRNA Translation: BAE39891.1
BC086683 mRNA Translation: AAH86683.1
BC116238 mRNA Translation: AAI16239.1
CCDSiCCDS40554.1 [Q9D083-1]
RefSeqiNP_080558.1, NM_026282.5 [Q9D083-1]
UniGeneiMm.295642

3D structure databases

ProteinModelPortaliQ9D083
SMRiQ9D083
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-551 Ndc80 complex
IntActiQ9D083, 1 interactor
MINTiQ9D083
STRINGi10090.ENSMUSP00000096541

PTM databases

iPTMnetiQ9D083
PhosphoSitePlusiQ9D083

Proteomic databases

EPDiQ9D083
PaxDbiQ9D083
PeptideAtlasiQ9D083
PRIDEiQ9D083

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098942; ENSMUSP00000096541; ENSMUSG00000074476 [Q9D083-1]
ENSMUST00000217382; ENSMUSP00000150475; ENSMUSG00000074476 [Q9D083-3]
GeneIDi67629
KEGGimmu:67629
UCSCiuc009oml.1 mouse [Q9D083-1]

Organism-specific databases

CTDi147841
MGIiMGI:1914879 Spc24

Phylogenomic databases

eggNOGiENOG410IYQI Eukaryota
ENOG4111TPD LUCA
GeneTreeiENSGT00390000005584
HOGENOMiHOG000004855
HOVERGENiHBG083027
InParanoidiQ9D083
KOiK11549
OMAiGMVKGIH
OrthoDBiEOG091G0N4A
PhylomeDBiQ9D083
TreeFamiTF333217

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:Q9D083
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074476 Expressed in 172 organ(s), highest expression level in pes
ExpressionAtlasiQ9D083 baseline and differential
GenevisibleiQ9D083 MM

Family and domain databases

InterProiView protein in InterPro
IPR013252 Ndc80_Spc24
PANTHERiPTHR22142 PTHR22142, 1 hit
PfamiView protein in Pfam
PF08286 Spc24, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPC24_MOUSE
AccessioniPrimary (citable) accession number: Q9D083
Secondary accession number(s): Q3TIF5, Q8CD13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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