UniProtKB - Q9D074 (MGRN1_MOUSE)
E3 ubiquitin-protein ligase MGRN1
Mgrn1
Functioni
E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity).
Acts also as a negative regulator of hedgehog signaling (PubMed:29290584).
By similarity3 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 277 – 316 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- endosome to lysosome transport Source: MGI
- negative regulation of cAMP-mediated signaling Source: UniProtKB
- negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of smoothened signaling pathway Source: UniProtKB
- protein monoubiquitination Source: MGI
- protein polyubiquitination Source: MGI
- protein ubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase MGRN1 (EC:2.3.2.27)Alternative name(s): Mahogunin RING finger protein 1 RING-type E3 ubiquitin transferase MGRN1Curated |
Gene namesi | Name:Mgrn1 Synonyms:Kiaa0544 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2447670, Mgrn1 |
VEuPathDBi | HostDB:ENSMUSG00000022517 |
Subcellular locationi
Endosome
- Early endosome 2 Publications
Note: The endosomal localization is dependent on the interaction with TSG101.By similarity
Cytoplasm and Cytosol
Plasma membrane
- Cell membrane By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Endosome
- early endosome Source: MGI
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endosome, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 278 – 281 | CVVC → AVVA: Loss of ubiquitin-protein ligase activity. Increase in TSG101-binding. 1 Publication | 4 | |
Mutagenesisi | 384 – 387 | PSAP → ASAA: Loss of TSG101-binding. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000246688 | 2 – 532 | E3 ubiquitin-protein ligase MGRN1Add BLAST | 531 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 389 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 428 | PhosphoserineCombined sources | 1 | |
Modified residuei | 449 | PhosphoserineCombined sources | 1 | |
Modified residuei | 452 | PhosphoserineCombined sources | 1 | |
Modified residuei | 501 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9D074 |
MaxQBi | Q9D074 |
PaxDbi | Q9D074 |
PeptideAtlasi | Q9D074 |
PRIDEi | Q9D074 |
ProteomicsDBi | 293471 [Q9D074-1] 293472 [Q9D074-2] 293473 [Q9D074-3] 293474 [Q9D074-4] 293475 [Q9D074-5] |
PTM databases
iPTMneti | Q9D074 |
PhosphoSitePlusi | Q9D074 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022517, Expressed in cerebellum and 277 other tissues |
Genevisiblei | Q9D074, MM |
Interactioni
Subunit structurei
Interacts with MC1R and MC4R (By similarity).
Interacts with TSG101.
Interacts with mislocalized cytosolically exposed PRNP; this interaction alters MGRN1 subcellular location and causes lysosomal enlargement.
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 201365, 4 interactors |
STRINGi | 10090.ENSMUSP00000023159 |
Miscellaneous databases
RNActi | Q9D074, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 419 – 518 | DisorderedSequence analysisAdd BLAST | 100 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 384 – 387 | Required for TSG101-binding | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 419 – 435 | Polar residuesSequence analysisAdd BLAST | 17 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 277 – 316 | RING-typePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4265, Eukaryota |
GeneTreei | ENSGT00390000009925 |
HOGENOMi | CLU_016631_1_1_1 |
InParanoidi | Q9D074 |
OMAi | CAQSGPP |
OrthoDBi | 883624at2759 |
PhylomeDBi | Q9D074 |
TreeFami | TF314969 |
Family and domain databases
CDDi | cd16789, mRING-HC-C3HC5_MGRN1_like---blasttree, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR045194, MGRN1/RNF157-like IPR045195, MGRN1/RNF157_mRING_C3HC5 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR22996, PTHR22996, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSILSRRIA GVEDIDIQAN SAYRYPPKSG NYFASHFFMG GEKFDTPHPE
60 70 80 90 100
GYLFGENMDL NFLGSRPVQF PYVTPAPHEP VKTLRSLVNI RKDSLRLVRY
110 120 130 140 150
KEDADSPTED GEKPRVLYSL EFTFDADARV AITIYCQAVE ELVNGVAVYS
160 170 180 190 200
CKNPSLQSET VHYKRGVSQQ FSLPSFKIDF SEWKDDELNF DLDRGVFPVV
210 220 230 240 250
IQAVVDEGDV VEVTGHAHVL LAAFEKHVDG SFSVKPLKQK QIVDRVSYLL
260 270 280 290 300
QEIYGIENKN NQETKPSDDE NSDNSSECVV CLSDLRDTLI LPCRHLCLCT
310 320 330 340 350
SCADTLRYQA NNCPICRLPF RALLQIRAVR KKPGALSPIS FSPVLAQSVD
360 370 380 390 400
HDEHSSSDSI PPGYEPISLL EALNGLRAVS PAIPSAPLYE EITYSGISDG
410 420 430 440 450
LSQASCPLAG LDRIMESGLQ KGKTQSKSPD STLRSPSFPI HEEDEEKLSE
460 470 480 490 500
DSDAPLPPSG VELVLRESSS PESFGTEEGD EPSLKQGSRV PSIDDVLQDG
510 520 530
SPQHHGCSQP VPPADIYLPA LGPESCSVGI EE
The sequence of this isoform differs from the canonical sequence as follows:
520-532: ALGPESCSVGIEE → GWSTSMETPHSLGTTSSPWPLLSGSSPEPGVAELTPF
The sequence of this isoform differs from the canonical sequence as follows:
355-355: S → SCPFKKSKSHPASLASKKPKRET
520-532: ALGPESCSVGIEE → GWSTSMETPHSLGTTSSPWPLLSGSSPEPGVAELTPF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | I → F in BAE29360 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | N → D in BAE29360 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 36 | H → R in BAE29360 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019854 | 29 | S → SA in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_019855 | 355 | S → SCPFKKSKSHPASLASKKPK RET in isoform 4 and isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_019856 | 520 – 532 | ALGPE…VGIEE → GWSTSMETPHSLGTTSSPWP LLSGSSPEPGVAELTPF in isoform 3 and isoform 5. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011747 mRNA Translation: BAB27816.2 AK034100 mRNA Translation: BAC28587.1 AK088533 mRNA Translation: BAC40408.1 AK150176 mRNA Translation: BAE29360.1 AK153407 mRNA Translation: BAE31967.1 BC046830 mRNA Translation: AAH46830.1 AK129161 mRNA Translation: BAC97971.1 |
CCDSi | CCDS49751.1 [Q9D074-1] CCDS57016.1 [Q9D074-2] CCDS88868.1 [Q9D074-4] CCDS88869.1 [Q9D074-3] |
RefSeqi | NP_001239366.1, NM_001252437.1 [Q9D074-2] NP_083933.1, NM_029657.4 [Q9D074-1] XP_006521895.1, XM_006521832.1 XP_006521897.1, XM_006521834.1 XP_017172376.1, XM_017316887.1 |
Genome annotation databases
Ensembli | ENSMUST00000023159; ENSMUSP00000023159; ENSMUSG00000022517 [Q9D074-3] ENSMUST00000070658; ENSMUSP00000068314; ENSMUSG00000022517 [Q9D074-1] ENSMUST00000229038; ENSMUSP00000155034; ENSMUSG00000022517 [Q9D074-2] ENSMUST00000230990; ENSMUSP00000155425; ENSMUSG00000022517 [Q9D074-4] |
GeneIDi | 17237 |
KEGGi | mmu:17237 |
UCSCi | uc007yap.2, mouse [Q9D074-1] uc007yaq.2, mouse [Q9D074-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK011747 mRNA Translation: BAB27816.2 AK034100 mRNA Translation: BAC28587.1 AK088533 mRNA Translation: BAC40408.1 AK150176 mRNA Translation: BAE29360.1 AK153407 mRNA Translation: BAE31967.1 BC046830 mRNA Translation: AAH46830.1 AK129161 mRNA Translation: BAC97971.1 |
CCDSi | CCDS49751.1 [Q9D074-1] CCDS57016.1 [Q9D074-2] CCDS88868.1 [Q9D074-4] CCDS88869.1 [Q9D074-3] |
RefSeqi | NP_001239366.1, NM_001252437.1 [Q9D074-2] NP_083933.1, NM_029657.4 [Q9D074-1] XP_006521895.1, XM_006521832.1 XP_006521897.1, XM_006521834.1 XP_017172376.1, XM_017316887.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 201365, 4 interactors |
STRINGi | 10090.ENSMUSP00000023159 |
PTM databases
iPTMneti | Q9D074 |
PhosphoSitePlusi | Q9D074 |
Proteomic databases
jPOSTi | Q9D074 |
MaxQBi | Q9D074 |
PaxDbi | Q9D074 |
PeptideAtlasi | Q9D074 |
PRIDEi | Q9D074 |
ProteomicsDBi | 293471 [Q9D074-1] 293472 [Q9D074-2] 293473 [Q9D074-3] 293474 [Q9D074-4] 293475 [Q9D074-5] |
Protocols and materials databases
Antibodypediai | 2013, 243 antibodies from 28 providers |
DNASUi | 17237 |
Genome annotation databases
Ensembli | ENSMUST00000023159; ENSMUSP00000023159; ENSMUSG00000022517 [Q9D074-3] ENSMUST00000070658; ENSMUSP00000068314; ENSMUSG00000022517 [Q9D074-1] ENSMUST00000229038; ENSMUSP00000155034; ENSMUSG00000022517 [Q9D074-2] ENSMUST00000230990; ENSMUSP00000155425; ENSMUSG00000022517 [Q9D074-4] |
GeneIDi | 17237 |
KEGGi | mmu:17237 |
UCSCi | uc007yap.2, mouse [Q9D074-1] uc007yaq.2, mouse [Q9D074-2] |
Organism-specific databases
CTDi | 23295 |
MGIi | MGI:2447670, Mgrn1 |
VEuPathDBi | HostDB:ENSMUSG00000022517 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG4265, Eukaryota |
GeneTreei | ENSGT00390000009925 |
HOGENOMi | CLU_016631_1_1_1 |
InParanoidi | Q9D074 |
OMAi | CAQSGPP |
OrthoDBi | 883624at2759 |
PhylomeDBi | Q9D074 |
TreeFami | TF314969 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 17237, 3 hits in 64 CRISPR screens |
ChiTaRSi | Mgrn1, mouse |
PROi | PR:Q9D074 |
RNActi | Q9D074, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022517, Expressed in cerebellum and 277 other tissues |
Genevisiblei | Q9D074, MM |
Family and domain databases
CDDi | cd16789, mRING-HC-C3HC5_MGRN1_like---blasttree, 1 hit |
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR045194, MGRN1/RNF157-like IPR045195, MGRN1/RNF157_mRING_C3HC5 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR22996, PTHR22996, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MGRN1_MOUSE | |
Accessioni | Q9D074Primary (citable) accession number: Q9D074 Secondary accession number(s): Q3U5V9 Q8BZM9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways