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Protein

Twist-related protein 2

Gene

Twist2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors (By similarity).By similarity3 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • DNA-binding transcription factor activity Source: MGI
  • protein dimerization activity Source: InterPro
  • protein domain specific binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processDifferentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Twist-related protein 2
Alternative name(s):
Dermis-expressed protein 1
Short name:
Dermo-1
Gene namesi
Name:Twist2
Synonyms:Dermo1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104685 Twist2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi86F → P: Abrogates transcriptional repression of MYOD1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274901 – 160Twist-related protein 2Add BLAST160

Proteomic databases

PaxDbiQ9D030
PRIDEiQ9D030

PTM databases

iPTMnetiQ9D030
PhosphoSitePlusiQ9D030

Expressioni

Tissue specificityi

Expressed at low levels in sclerotome and dermatome of somites, and in limb buds at 10.5 dpc. Accumulates predominantly in dermatome, prevertebrae and derivatives of branchial arches by 13 dpc. Also expressed near surface of embryo and in chondrogenic cells. In adult, expressed at low levels in skin, bladder, uterus, aorta and heart.1 Publication

Developmental stagei

In the embryo, expression is detected at 10.5 dpc, increases continuously through to 17.5 dpc and is also high in neonates. Down-regulated in adults.1 Publication

Inductioni

By TNF-alpha.1 Publication

Gene expression databases

CleanExiMM_TWIST2

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Runx2Q087752EBI-2903190,EBI-903354

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199209, 1 interactor
IntActiQ9D030, 1 interactor
STRINGi10090.ENSMUSP00000007949

Structurei

3D structure databases

ProteinModelPortaliQ9D030
SMRiQ9D030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 117bHLHPROSITE-ProRule annotationAdd BLAST52

Domaini

The C-terminal and HLH domains are essential for transcriptional repression.1 Publication

Phylogenomic databases

eggNOGiKOG4447 Eukaryota
ENOG4111QFU LUCA
HOGENOMiHOG000261629
HOVERGENiHBG019071
InParanoidiQ9D030
KOiK09069
PhylomeDBiQ9D030

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9D030-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEGSSSPVS PVDSLGTSEE ELERQPKRFG RKRRYSKKSS EDGSPTPGKR
60 70 80 90 100
GKKGSPSAQS FEELQSQRIL ANVRERQRTQ SLNEAFAALR KIIPTLPSDK
110 120 130 140 150
LSKIQTLKLA ARYIDFLYQV LQSDEMDNKM TSCSYVAHER LSYAYSVWRM
160
EGAWSMSASH
Length:160
Mass (Da):18,140
Last modified:June 1, 2001 - v1
Checksum:i6394750916940C12
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A5D6P6A5D6P6_MOUSE
Twist homolog 2 (Drosophila)
Twist2 mCG_20120
160Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145Y → F in BAB27885 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36384 mRNA Translation: AAC52319.1
AK011861 mRNA Translation: BAB27885.1
AK011180 mRNA Translation: BAB27450.1
RefSeqiNP_031881.1, NM_007855.3
UniGeneiMm.9474

Genome annotation databases

GeneIDi13345
KEGGimmu:13345

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36384 mRNA Translation: AAC52319.1
AK011861 mRNA Translation: BAB27885.1
AK011180 mRNA Translation: BAB27450.1
RefSeqiNP_031881.1, NM_007855.3
UniGeneiMm.9474

3D structure databases

ProteinModelPortaliQ9D030
SMRiQ9D030
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199209, 1 interactor
IntActiQ9D030, 1 interactor
STRINGi10090.ENSMUSP00000007949

PTM databases

iPTMnetiQ9D030
PhosphoSitePlusiQ9D030

Proteomic databases

PaxDbiQ9D030
PRIDEiQ9D030

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13345
KEGGimmu:13345

Organism-specific databases

CTDi117581
MGIiMGI:104685 Twist2

Phylogenomic databases

eggNOGiKOG4447 Eukaryota
ENOG4111QFU LUCA
HOGENOMiHOG000261629
HOVERGENiHBG019071
InParanoidiQ9D030
KOiK09069
PhylomeDBiQ9D030

Miscellaneous databases

PROiPR:Q9D030
SOURCEiSearch...

Gene expression databases

CleanExiMM_TWIST2

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTWST2_MOUSE
AccessioniPrimary (citable) accession number: Q9D030
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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