UniProtKB - Q9CZX0 (ELP3_MOUSE)
Elongator complex protein 3
Elp3
Functioni
Caution
Catalytic activityi
- acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine34 in tRNA = 5'-deoxyadenosine + carboxymethyluridine34 in tRNA + CoA + 2 H+ + L-methionineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Cofactori
: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.By similarityView all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 99 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 109 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Metal bindingi | 112 | Iron-sulfur (4Fe-4S-S-AdoMet)By similarity | 1 | |
Binding sitei | 164 | Acetyl-CoABy similarity | 1 | |
Binding sitei | 530 | Acetyl-CoABy similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- acetyltransferase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
- N-acetyltransferase activity Source: InterPro
- phosphorylase kinase regulator activity Source: UniProtKB
- tRNA binding Source: UniProtKB-KW
- tRNA uridine(34) acetyltransferase activity Source: UniProtKB
GO - Biological processi
- central nervous system development Source: UniProtKB
- neuron migration Source: UniProtKB
- positive regulation of cell migration Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: UniProtKB
- tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation Source: GO_Central
- tRNA wobble uridine modification Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | Neurogenesis, Transcription, Transcription regulation, tRNA processing |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
UniPathwayi | UPA00988 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1921445, Elp3 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
Other locations
- cytoplasm Source: UniProtKB
- Elongator holoenzyme complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000283987 | 1 – 547 | Elongator complex protein 3Add BLAST | 547 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 161 | PhosphoserineCombined sources | 1 | |
Modified residuei | 229 | N6-methyllysineBy similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q9CZX0 |
PaxDbi | Q9CZX0 |
PeptideAtlasi | Q9CZX0 |
PRIDEi | Q9CZX0 |
PTM databases
iPTMneti | Q9CZX0 |
PhosphoSitePlusi | Q9CZX0 |
SwissPalmi | Q9CZX0 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022031, Expressed in forelimb bud and 297 other tissues |
ExpressionAtlasi | Q9CZX0, baseline and differential |
Genevisiblei | Q9CZX0, MM |
Interactioni
Subunit structurei
Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. ELP1, STIP1/ELP2 and ELP3 form the Elongator core complex. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit.
Interacts with ELP1.
Interacts with alpha-tubulin.
By similarityProtein-protein interaction databases
BioGRIDi | 216566, 23 interactors |
IntActi | Q9CZX0, 3 interactors |
MINTi | Q9CZX0 |
STRINGi | 10090.ENSMUSP00000022609 |
Miscellaneous databases
RNActi | Q9CZX0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 372 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 291 | |
Domaini | 396 – 547 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 152 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 474 – 477 | Acetyl-CoA bindingBy similarity | 4 | |
Regioni | 497 – 499 | Acetyl-CoA bindingBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2535, Eukaryota |
GeneTreei | ENSGT00390000013141 |
HOGENOMi | CLU_025983_2_1_1 |
InParanoidi | Q9CZX0 |
OMAi | TFETRPD |
OrthoDBi | 320059at2759 |
PhylomeDBi | Q9CZX0 |
TreeFami | TF105752 |
Family and domain databases
Gene3Di | 3.80.30.20, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR039661, ELP3 IPR034687, ELP3-like IPR006638, Elp3/MiaB/NifB IPR000182, GNAT_dom IPR032432, Radical_SAM_C IPR007197, rSAM IPR023404, rSAM_horseshoe |
PANTHERi | PTHR11135, PTHR11135, 1 hit PTHR11135:SF0, PTHR11135:SF0, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit PF04055, Radical_SAM, 1 hit PF16199, Radical_SAM_C, 1 hit |
PIRSFi | PIRSF005669, Hist_AcTrfase_ELP3, 1 hit |
SFLDi | SFLDF00344, ELP3-like, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01211, ELP3, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit PS51918, RADICAL_SAM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRQKRKGDLS PAELMMLTIG DVIKQLVEAH EQGKDVDLNK MKTKTAAKYG
60 70 80 90 100
LASQPRLVDI IAAVPPHYRK ILIPKLKAKP VRTASGIAVV AVMCKPHRCP
110 120 130 140 150
HISFTGNICI YCPGGPDSDF EYSTQSYTGY EPTSMRAIRA RYDPFLQTRH
160 170 180 190 200
RIEQLKQLGH SVDKVEFIVM GGTFMALPEE YRDYFIRSLH DALSGHTSNN
210 220 230 240 250
IHEAIKYSER SFTKCVGITI ETRPDYCMKR HLSDMLTYGC TRLEIGVQSV
260 270 280 290 300
YEDVARDTNR GHTVKAACES FHLAKDSGFK VVTHMMPDLP NVGLERDIEQ
310 320 330 340 350
FIEFFENPAF RPDGLKLYPT LVIRGTGLYE LWKSGRYRSY SPSDLIELVA
360 370 380 390 400
RILALVPPWT RVYRVQRDIP MPLVSSGVEH GNLRELAFAR MKDLGIQCRD
410 420 430 440 450
VRTREVGIQE IHHRVRPYQV ELVRRDYVAN GGWETFLSYE DPDQDILIGL
460 470 480 490 500
LRLRKCSEET FRFELGGGVS IVRELHVYGS VVPVSSRDPT KFQHQGFGML
510 520 530 540
LMEEAERIAR EEHGSGKMAV ISGVGTRNYY RKIGYRLQGP YMVKMLK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A286YDB8 | A0A286YDB8_MOUSE | Elongator complex protein 3 | Elp3 | 553 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024408 | 7 | G → GAKYVGQGRKGGSGFSEITG in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK012072 mRNA Translation: BAB28009.1 AK088457 mRNA Translation: BAC40364.1 BC026461 mRNA Translation: AAH26461.1 BC057453 mRNA Translation: AAH57453.1 |
CCDSi | CCDS56965.1 [Q9CZX0-2] CCDS88691.1 [Q9CZX0-1] |
RefSeqi | NP_001240741.1, NM_001253812.1 [Q9CZX0-2] NP_083087.1, NM_028811.3 [Q9CZX0-1] |
Genome annotation databases
Ensembli | ENSMUST00000022609; ENSMUSP00000022609; ENSMUSG00000022031 [Q9CZX0-2] ENSMUST00000225355; ENSMUSP00000153462; ENSMUSG00000022031 [Q9CZX0-1] |
GeneIDi | 74195 |
KEGGi | mmu:74195 |
UCSCi | uc007ujk.2, mouse [Q9CZX0-1] uc007ujl.2, mouse [Q9CZX0-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK012072 mRNA Translation: BAB28009.1 AK088457 mRNA Translation: BAC40364.1 BC026461 mRNA Translation: AAH26461.1 BC057453 mRNA Translation: AAH57453.1 |
CCDSi | CCDS56965.1 [Q9CZX0-2] CCDS88691.1 [Q9CZX0-1] |
RefSeqi | NP_001240741.1, NM_001253812.1 [Q9CZX0-2] NP_083087.1, NM_028811.3 [Q9CZX0-1] |
3D structure databases
SMRi | Q9CZX0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216566, 23 interactors |
IntActi | Q9CZX0, 3 interactors |
MINTi | Q9CZX0 |
STRINGi | 10090.ENSMUSP00000022609 |
PTM databases
iPTMneti | Q9CZX0 |
PhosphoSitePlusi | Q9CZX0 |
SwissPalmi | Q9CZX0 |
Proteomic databases
EPDi | Q9CZX0 |
PaxDbi | Q9CZX0 |
PeptideAtlasi | Q9CZX0 |
PRIDEi | Q9CZX0 |
Protocols and materials databases
Antibodypediai | 23089, 206 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022609; ENSMUSP00000022609; ENSMUSG00000022031 [Q9CZX0-2] ENSMUST00000225355; ENSMUSP00000153462; ENSMUSG00000022031 [Q9CZX0-1] |
GeneIDi | 74195 |
KEGGi | mmu:74195 |
UCSCi | uc007ujk.2, mouse [Q9CZX0-1] uc007ujl.2, mouse [Q9CZX0-2] |
Organism-specific databases
CTDi | 55140 |
MGIi | MGI:1921445, Elp3 |
Phylogenomic databases
eggNOGi | KOG2535, Eukaryota |
GeneTreei | ENSGT00390000013141 |
HOGENOMi | CLU_025983_2_1_1 |
InParanoidi | Q9CZX0 |
OMAi | TFETRPD |
OrthoDBi | 320059at2759 |
PhylomeDBi | Q9CZX0 |
TreeFami | TF105752 |
Enzyme and pathway databases
UniPathwayi | UPA00988 |
Miscellaneous databases
BioGRID-ORCSi | 74195, 10 hits in 21 CRISPR screens |
ChiTaRSi | Elp3, mouse |
PROi | PR:Q9CZX0 |
RNActi | Q9CZX0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022031, Expressed in forelimb bud and 297 other tissues |
ExpressionAtlasi | Q9CZX0, baseline and differential |
Genevisiblei | Q9CZX0, MM |
Family and domain databases
Gene3Di | 3.80.30.20, 1 hit |
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR039661, ELP3 IPR034687, ELP3-like IPR006638, Elp3/MiaB/NifB IPR000182, GNAT_dom IPR032432, Radical_SAM_C IPR007197, rSAM IPR023404, rSAM_horseshoe |
PANTHERi | PTHR11135, PTHR11135, 1 hit PTHR11135:SF0, PTHR11135:SF0, 1 hit |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit PF04055, Radical_SAM, 1 hit PF16199, Radical_SAM_C, 1 hit |
PIRSFi | PIRSF005669, Hist_AcTrfase_ELP3, 1 hit |
SFLDi | SFLDF00344, ELP3-like, 1 hit |
SMARTi | View protein in SMART SM00729, Elp3, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
TIGRFAMsi | TIGR01211, ELP3, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit PS51918, RADICAL_SAM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELP3_MOUSE | |
Accessioni | Q9CZX0Primary (citable) accession number: Q9CZX0 Secondary accession number(s): Q8C2K2, Q8R369 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families