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Protein

Citrate synthase, mitochondrial

Gene

Cs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (Cs)
  2. Aconitate hydratase, mitochondrial (Aco2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei301PROSITE-ProRule annotation1
Active sitei347PROSITE-ProRule annotation1
Active sitei402PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-MMU-71403 Citric acid cycle (TCA cycle)
UniPathwayi
UPA00223;UER00717

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, mitochondrial (EC:2.3.3.1)
Alternative name(s):
Citrate (Si)-synthase
Gene namesi
Name:Cs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:88529 Cs

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176798

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000547228 – 464Citrate synthase, mitochondrialAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei76N6-acetyllysine; alternateBy similarity1
Modified residuei76N6-succinyllysine; alternateBy similarity1
Modified residuei103N6-succinyllysineCombined sources1
Modified residuei193N6-succinyllysineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei321N6-acetyllysine; alternateCombined sources1
Modified residuei321N6-succinyllysine; alternateCombined sources1
Modified residuei327N6-acetyllysine; alternateCombined sources1
Modified residuei327N6-succinyllysine; alternateCombined sources1
Modified residuei375N6-acetyllysine; alternateCombined sources1
Modified residuei375N6-succinyllysine; alternateCombined sources1
Modified residuei382N6-acetyllysineCombined sources1
Modified residuei393N6-acetyllysine; alternateCombined sources1
Modified residuei393N6-succinyllysine; alternateCombined sources1
Modified residuei395N6,N6,N6-trimethyllysineBy similarity1
Modified residuei450N6-succinyllysineCombined sources1
Modified residuei459N6-acetyllysine; alternateCombined sources1
Modified residuei459N6-succinyllysine; alternateCombined sources1

Post-translational modificationi

Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9CZU6
MaxQBiQ9CZU6
PaxDbiQ9CZU6
PeptideAtlasiQ9CZU6
PRIDEiQ9CZU6

PTM databases

iPTMnetiQ9CZU6
PhosphoSitePlusiQ9CZU6
SwissPalmiQ9CZU6

Expressioni

Gene expression databases

BgeeiENSMUSG00000005683 Expressed in 311 organ(s), highest expression level in intestinal epithelium
CleanExiMM_CS
GenevisibleiQ9CZU6 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198926, 2 interactors
IntActiQ9CZU6, 9 interactors
MINTiQ9CZU6
STRINGi10090.ENSMUSP00000005826

Structurei

3D structure databases

ProteinModelPortaliQ9CZU6
SMRiQ9CZU6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
GeneTreeiENSGT00390000006813
HOGENOMiHOG000130831
HOVERGENiHBG005336
InParanoidiQ9CZU6
KOiK01647
OMAiTVGWCAQ
OrthoDBiEOG091G068F
PhylomeDBiQ9CZU6
TreeFamiTF300398

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CZU6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALLTAATRL LGAKNSSCLV LAARHASASS TNLKDVLSNL IPKEQARIKT
60 70 80 90 100
FKQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE
110 120 130 140 150
CQKMLPKAKG GEEPLPEGLF WLLVTGQMPT EEQVSWLSRE WAKRAALPSH
160 170 180 190 200
VVTMLDNFPT NLHPMSQLSA AITALNSESN FARAYAEGMN RAKYWELIYE
210 220 230 240 250
DCMDLIAKLP CVAAKIYRNL YREGSSIGAI DSRLDWSHNF TNMLGYTDPQ
260 270 280 290 300
FTELMRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL
310 320 330 340 350
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
360 370 380 390 400
RKTDPRYSCQ REFALKHLPK DPMFKLVAQL YKIVPNILLE QGKAKNPWPN
410 420 430 440 450
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
460
SMSTDGLMKF VDSK
Length:464
Mass (Da):51,737
Last modified:June 1, 2001 - v1
Checksum:i710639871C31EED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056479 mRNA Translation: BAB63945.1
AK012151 mRNA Translation: BAB28063.1
AK145643 mRNA Translation: BAE26560.1
AK149990 mRNA Translation: BAE29218.1
AK163273 mRNA Translation: BAE37268.1
AK166814 mRNA Translation: BAE39041.1
AK167125 mRNA Translation: BAE39273.1
BC013554 mRNA Translation: AAH13554.1
BC029754 mRNA Translation: AAH29754.1
CCDSiCCDS24273.1
RefSeqiNP_080720.1, NM_026444.4
UniGeneiMm.58836

Genome annotation databases

EnsembliENSMUST00000005826; ENSMUSP00000005826; ENSMUSG00000005683
GeneIDi12974
KEGGimmu:12974
UCSCiuc007hmj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056479 mRNA Translation: BAB63945.1
AK012151 mRNA Translation: BAB28063.1
AK145643 mRNA Translation: BAE26560.1
AK149990 mRNA Translation: BAE29218.1
AK163273 mRNA Translation: BAE37268.1
AK166814 mRNA Translation: BAE39041.1
AK167125 mRNA Translation: BAE39273.1
BC013554 mRNA Translation: AAH13554.1
BC029754 mRNA Translation: AAH29754.1
CCDSiCCDS24273.1
RefSeqiNP_080720.1, NM_026444.4
UniGeneiMm.58836

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NZIX-ray2.75B371-377[»]
ProteinModelPortaliQ9CZU6
SMRiQ9CZU6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198926, 2 interactors
IntActiQ9CZU6, 9 interactors
MINTiQ9CZU6
STRINGi10090.ENSMUSP00000005826

Chemistry databases

ChEMBLiCHEMBL2176798

PTM databases

iPTMnetiQ9CZU6
PhosphoSitePlusiQ9CZU6
SwissPalmiQ9CZU6

Proteomic databases

EPDiQ9CZU6
MaxQBiQ9CZU6
PaxDbiQ9CZU6
PeptideAtlasiQ9CZU6
PRIDEiQ9CZU6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005826; ENSMUSP00000005826; ENSMUSG00000005683
GeneIDi12974
KEGGimmu:12974
UCSCiuc007hmj.1 mouse

Organism-specific databases

CTDi1431
MGIiMGI:88529 Cs

Phylogenomic databases

eggNOGiKOG2617 Eukaryota
COG0372 LUCA
GeneTreeiENSGT00390000006813
HOGENOMiHOG000130831
HOVERGENiHBG005336
InParanoidiQ9CZU6
KOiK01647
OMAiTVGWCAQ
OrthoDBiEOG091G068F
PhylomeDBiQ9CZU6
TreeFamiTF300398

Enzyme and pathway databases

UniPathwayi
UPA00223;UER00717

ReactomeiR-MMU-71403 Citric acid cycle (TCA cycle)

Miscellaneous databases

ChiTaRSiCs mouse
PROiPR:Q9CZU6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005683 Expressed in 311 organ(s), highest expression level in intestinal epithelium
CleanExiMM_CS
GenevisibleiQ9CZU6 MM

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR010109 Citrate_synthase_euk
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01793 cit_synth_euk, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCISY_MOUSE
AccessioniPrimary (citable) accession number: Q9CZU6
Secondary accession number(s): Q3UDP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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